Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,783	T→G	D133E (GAT→GAG)	yhgF →	putative RNA‑binding protein YhgF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,783	0	T	G	100.0%	238.4 / NA	60	D133E (GAT→GAG)	yhgF	putative RNA‑binding protein YhgF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (27/33);  total (27/33)

GCGAAGGCCATCAACGCCACCCTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGA  >  NC_000913/3537646‑3537919                                                                                                                                          |                                                                                                                                         gCGAAGGCCATCAACGCCACCCTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGcc                                                                                                                                        >  2:300128/1‑140 (MQ=255)       gCCATCAACGCCACCCTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTcaca                                                                                                                                  <  1:132465/140‑1 (MQ=255)       gCCATCAACGCCACCCTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTcaca                                                                                                                                  <  2:90652/140‑1 (MQ=255)         cATCAACGCCACCCTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTcacaca                                                                                                                                <  1:750143/140‑1 (MQ=255)            cAACGCCACCCTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGc                                                                                                                             <  1:490795/140‑1 (MQ=255)                     ccTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGt                                                                                                                      >  2:997342/1‑138 (MQ=255)                     ccTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGt                                                                                                                      <  1:997342/138‑1 (MQ=255)                     ccTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGC‑CCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTcgc                                                                                                                   >  2:23778/1‑140 (MQ=255)                      cTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTcgc                                                                                                                   <  2:617717/140‑1 (MQ=255)                         aGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTcgccgc                                                                                                                >  2:396907/1‑140 (MQ=255)                           cAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTg                                                                                                              <  1:271725/140‑1 (MQ=255)                              aaCCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTg                                                                                                              <  2:507654/137‑1 (MQ=255)                              aaCCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTg                                                                                                              >  1:507654/1‑137 (MQ=255)                               aCCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGcaca                                                                                                          <  1:248852/140‑1 (MQ=255)                                ccGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACaa                                                                                                         <  1:476103/140‑1 (MQ=255)                                ccGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACaa                                                                                                         <  1:76393/140‑1 (MQ=255)                                 cGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAat                                                                                                        <  1:483613/140‑1 (MQ=255)                                   aaCTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCt                                                                                                               >  1:525713/1‑131 (MQ=255)                                   aaCTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCt                                                                                                               <  2:525713/131‑1 (MQ=255)                                   aaCTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAatat                                                                                                      >  1:1011069/1‑140 (MQ=255)                                          aGACCTCTACCTGCCCTACAAACCTAAACGCCGC‑CCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGc                                                                                              <  1:23778/140‑1 (MQ=255)                                                   ccTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAg                                                                                      <  2:974038/140‑1 (MQ=255)                                                     tGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGc                                                                                    <  1:396907/140‑1 (MQ=255)                                                      gCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCg                                                                                   <  1:104226/140‑1 (MQ=255)                                                       cccTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGt                                                                                  >  1:1061887/1‑140 (MQ=255)                                                        ccTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTg                                                                                 >  2:160744/1‑140 (MQ=255)                                                        ccTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTg                                                                                 >  1:438525/1‑140 (MQ=255)                                                           aCAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCa                                                                              <  2:1011069/140‑1 (MQ=255)                                                                 cTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATAcc                                                                        >  1:193022/1‑140 (MQ=255)                                                                 cTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATAcc                                                                        >  2:512464/1‑140 (MQ=255)                                                                         cgcACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCcgc                                                                >  1:192366/1‑140 (MQ=255)                                                                         cgcACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCcgc                                                                >  2:910395/1‑140 (MQ=255)                                                                           cACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCgcgc                                                              >  2:792545/1‑140 (MQ=255)                                                                                   ggCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGgc                                                      >  2:458157/1‑140 (MQ=255)                                                                                     cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATggtgc                                                    <  2:192366/140‑4 (MQ=255)                                                                                     cAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGgcgc                                                    <  1:160744/140‑1 (MQ=255)                                                                                        aTCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGgcgcgcg                                                 <  1:512464/140‑1 (MQ=255)                                                                                        aTCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGgcgcgc                                                  <  2:171866/139‑1 (MQ=255)                                                                                        aTCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGgcgcgc                                                  >  1:171866/1‑139 (MQ=255)                                                                                              aTTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGgcgc                                                    >  1:380186/1‑131 (MQ=255)                                                                                              aTTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGgcgc                                                    <  2:380186/131‑1 (MQ=255)                                                                                              aTTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTatat                                           <  2:438525/140‑1 (MQ=255)                                                                                              aTTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTatat                                           <  1:458157/140‑1 (MQ=255)                                                                                                tGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATcc                                         <  1:910395/140‑1 (MQ=255)                                                                                                  aaGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTg                                       >  1:893982/1‑140 (MQ=255)                                                                                                       gggCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGa                                  >  1:920028/1‑140 (MQ=255)                                                                                                          cTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACg                               >  2:1079748/1‑140 (MQ=255)                                                                                                          cTTGAGCCGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACg                               >  1:991083/1‑140 (MQ=255)                                                                                                                ccGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGc                         <  2:167849/140‑1 (MQ=255)                                                                                                                ccGTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGc                         <  2:552884/140‑1 (MQ=255)                                                                                                                  gTTGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCg                       >  1:231881/1‑140 (MQ=255)                                                                                                                   ttGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGa                      >  1:667787/1‑140 (MQ=255)                                                                                                                   ttGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGa                      >  1:179944/1‑140 (MQ=255)                                                                                                                   ttGGCTGACCTGCTGTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGa                      >  1:1038926/1‑140 (MQ=255)                                                                                                                             tgctgTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCcgcg            <  2:283753/140‑1 (MQ=255)                                                                                                                             tgctgTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCcgcg            <  2:193022/140‑1 (MQ=255)                                                                                                                             tgctgTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCcgcg            <  2:920028/140‑1 (MQ=255)                                                                                                                             tgctgTGGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCcgcg            <  1:1079748/140‑1 (MQ=255)                                                                                                                                tgtgGAGCGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCgctg         >  1:980205/1‑140 (MQ=255)                                                                                                                                       cGAGCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGa  <  2:231881/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCGAAGGCCATCAACGCCACCCTAAGCAAAACCGAACTCGAAGACCTCTACCTGCCCTACAAACCTAAACGCCGCACCCGCGGGCAAATCGCCATTGAAGCAGGGCTTGAGCCGTTGGCTGACCTGCTGTGGAGCGATCCGTCACACACGCCAGAAGTCGCCGCTGCACAATATGTTTATGCCGATAAAGGCGTGGCAGATACCAAAGCCGCGCTGGATGGCGCGCGCTATATCCTGATGGAACGGTTTGCCGAAGATGCCGCGCTGCTGGCGA  >  NC_000913/3537646‑3537919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑