Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,525	A→T	V99E (GTG→GAG)	ilvY ←	DNA‑binding transcriptional dual regulator IlvY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,525	0	A	T	100.0%	240.7 / NA	69	V99E (GTG→GAG)	ilvY	DNA‑binding transcriptional dual regulator IlvY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (37/32);  total (37/32)

CCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCA  >  NC_000913/3957391‑3957657                                                                                                                                       |                                                                                                                                     ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAg                                                                                                                                 <  1:646611/140‑1 (MQ=255) ccAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAg                                                                                                                                 <  1:957919/140‑1 (MQ=255)    gATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCag                                                                                                                              <  2:216497/140‑1 (MQ=255)              aCCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGGGGTCTCCGAGCAGAAGATATGTa                                                                                                                    <  2:379327/140‑1 (MQ=255)               ccAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTaa                                                                                                                   >  2:831654/1‑140 (MQ=255)                     accaccTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGcc                                                                                                             >  1:873091/1‑140 (MQ=255)                            tttCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGTCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGc                                                                                                      >  1:552306/1‑140 (MQ=255)                             ttCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCg                                                                                                               >  2:453782/1‑130 (MQ=255)                             ttCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCg                                                                                                               <  1:453782/130‑1 (MQ=255)                                 aTCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACgg                                                                                                 >  2:681953/1‑140 (MQ=255)                                 aTCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACgg                                                                                                 >  2:884149/1‑140 (MQ=255)                                  tCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGc                                                                                                <  1:831654/140‑1 (MQ=255)                                    gCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCaga                                                                                                          >  2:698258/1‑128 (MQ=255)                                    gCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCaga                                                                                                          <  1:698258/128‑1 (MQ=255)                                       tCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTg                                                                                           <  1:270798/140‑1 (MQ=255)                                          gCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTg                                                                                        >  2:1080758/1‑140 (MQ=255)                                           ccGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGa                                                                                       >  2:812649/1‑140 (MQ=255)                                           ccGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGa                                                                                       >  1:366501/1‑140 (MQ=255)                                                tCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTg                                                                                           >  2:977462/1‑131 (MQ=255)                                                tCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTg                                                                                           <  1:977462/131‑1 (MQ=255)                                                tCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGAt                                                                                  >  1:814264/1‑140 (MQ=255)                                                     agtagtAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGgtgt                                                                             >  2:14934/1‑140 (MQ=255)                                                            aGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCaa                                                                      >  1:1069011/1‑140 (MQ=255)                                                              tttAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAAct                                                                    >  1:517372/1‑140 (MQ=255)                                                              tttAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAAct                                                                    >  2:542841/1‑140 (MQ=255)                                                              tttAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAAct                                                                    >  1:864019/1‑140 (MQ=255)                                                               ttAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActg                                                                   <  1:681953/140‑1 (MQ=255)                                                                 aaTCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgct                                                                 >  2:715503/1‑140 (MQ=255)                                                                 aaTCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgct                                                                 <  2:366501/140‑1 (MQ=255)                                                                 aaTCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgct                                                                 >  2:162736/1‑140 (MQ=255)                                                                 aaTCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgct                                                                 >  2:706245/1‑140 (MQ=255)                                                                 aaTCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAActgct                                                                 <  1:1080758/140‑1 (MQ=255)                                                                     tcCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATa                                                             <  2:873091/140‑1 (MQ=255)                                                                        aCCGACGGGTGTTCCGTGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGa                                                               >  2:868212/1‑135 (MQ=255)                                                                        aCCGACGGGTGTTCCGTGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGa                                                               <  1:868212/135‑1 (MQ=255)                                                                        aCCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTg                                                          >  2:465803/1‑140 (MQ=255)                                                                          cGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCa                                                        <  2:1069011/140‑1 (MQ=255)                                                                          cGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCa                                                        <  1:884149/140‑1 (MQ=255)                                                                           gACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCaa                                                       <  2:242731/140‑1 (MQ=255)                                                                              gggTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAg                                                    <  1:14934/140‑1 (MQ=255)                                                                                 tgtTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATa                                                             >  1:786286/1‑128 (MQ=255)                                                                                 tgtTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATa                                                             <  2:786286/128‑1 (MQ=255)                                                                                     ccGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGa                                                               >  2:467655/1‑122 (MQ=255)                                                                                     ccGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGa                                                               <  1:467655/122‑1 (MQ=255)                                                                                      cgcgcgGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCt                                            >  1:324424/1‑140 (MQ=255)                                                                                        cgcgGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTgg                                          >  2:283647/1‑140 (MQ=255)                                                                                                 gATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAAcg                                 <  1:812649/140‑1 (MQ=255)                                                                                                    ccAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACgcgc                              <  1:369978/140‑1 (MQ=255)                                                                                                       gAATCGGCGGCAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGc                           >  1:581914/1‑140 (MQ=255)                                                                                                             gcggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGaaa                                    >  1:322529/1‑125 (MQ=255)                                                                                                             gcggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGaaa                                    <  2:322529/125‑1 (MQ=255)                                                                                                             gcggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                     >  1:1077255/1‑140 (MQ=255)                                                                                                             gcggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                     >  1:344048/1‑140 (MQ=255)                                                                                                             gcggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                     >  1:636698/1‑140 (MQ=255)                                                                                                             gcggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                     >  1:782884/1‑140 (MQ=255)                                                                                                             gcggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCg                     >  1:16521/1‑140 (MQ=255)                                                                                                              cggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGc                    <  1:283647/140‑1 (MQ=255)                                                                                                              cggcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGc                    <  1:370605/140‑1 (MQ=255)                                                                                                                gcAGATGGCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGccc                  <  1:715503/140‑1 (MQ=255)                                                                                                                      ggCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtca            <  1:182240/140‑1 (MQ=255)                                                                                                                      ggCTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtca            <  1:1008907/140‑1 (MQ=255)                                                                                                                        cTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtcagt          <  2:782884/140‑1 (MQ=255)                                                                                                                        cTGTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtcagt          <  1:465803/140‑1 (MQ=255)                                                                                                                          gTAGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtcagtca        <  2:475047/140‑1 (MQ=255)                                                                                                                            aGGCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGc      >  2:62216/1‑140 (MQ=255)                                                                                                                             ggCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCg     <  2:655027/140‑1 (MQ=255)                                                                                                                             ggCAGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCg     >  1:655027/1‑140 (MQ=255)                                                                                                                                aGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtcagt          <  1:463004/132‑1 (MQ=255)                                                                                                                                aGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTtcagt          >  2:463004/1‑132 (MQ=255)                                                                                                                                aGCGGTCTCCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCa  >  1:433203/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     CCAGATCCGCTTCACCAGTGACCACCTTTTCCATCGCATCTGCCGCATCACCAGTAGTAAGTTTAATCTCCACCGACGGGTGTTCCGCGCGGAAGCGATCCAGAATCGGCGGCAGATGGCTGTAGGCAGCGGTCACCGAGCAGAAGATATGTAATTCGCCAGAGAGCGACGGCCCTTGCTGATCGATGGTGTGGCGCAACTGCTGATACTGCAACAGCGTTTGCTGGGCGAAAACGCGCAGCTCTTCGCCCGCTTCAGTCAGCGTCA  >  NC_000913/3957391‑3957657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑