Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,415,414	G→A	A380A (GCG→GCA)	aidB →	putative acyl‑CoA dehydrogenase AidB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,415,414	0	G	A	100.0%	217.1 / NA	61	A380A (GCG→GCA)	aidB	putative acyl‑CoA dehydrogenase AidB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/27);  total (34/27)

CCCTTATGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAA  >  NC_000913/4415278‑4415549                                                                                                                                         |                                                                                                                                        cccTTATGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTgcagca                                                                                                                                      >  1:44670/1‑140 (MQ=255)  ccTTATGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCaa                                                                                                                                     <  2:210036/140‑1 (MQ=255)   cTTATGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCaaa                                                                                                                                    >  2:348179/1‑140 (MQ=255)      aTGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAAtt                                                                                                                                 >  1:37968/1‑140 (MQ=255)       tGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCaa                                                                                                                                     <  2:844675/135‑1 (MQ=255)       tGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCaa                                                                                                                                     >  1:844675/1‑135 (MQ=255)          gTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTtgtg                                                                                                                             >  2:785318/1‑140 (MQ=255)          gTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTtgtg                                                                                                                             >  2:739555/1‑140 (MQ=255)           tCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGa                                                                                                                            >  1:537044/1‑140 (MQ=255)            cATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGAt                                                                                                                           <  2:752062/140‑1 (MQ=255)             aTGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATc                                                                                                                          >  1:8443/1‑140 (MQ=255)              tGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCt                                                                                                                         >  1:723490/1‑140 (MQ=255)                 cTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTg                                                                                                                        <  2:315556/138‑1 (MQ=255)                 cTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTg                                                                                                                        >  1:315556/1‑138 (MQ=255)                   tAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTgcagca                                                                                                                                      >  2:278602/1‑122 (MQ=255)                   tAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTgcagca                                                                                                                                      <  1:278602/122‑1 (MQ=255)                          cATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATc                                                                                                                          <  2:11958/127‑1 (MQ=255)                          cATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATc                                                                                                                          >  1:11958/1‑127 (MQ=255)                          cATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTa                                                                                                             >  2:584977/1‑140 (MQ=255)                          cATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTa                                                                                                             >  2:866008/1‑140 (MQ=255)                          cATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTa                                                                                                             >  1:1086604/1‑140 (MQ=255)                            tGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATg                                                                                                           <  2:537044/140‑1 (MQ=255)                               cACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCg                                                                                                        >  1:874006/1‑140 (MQ=255)                                          gAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGa                                                                                             <  2:44670/140‑1 (MQ=255)                                           aaGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGaa                                                                                            <  1:86085/140‑1 (MQ=255)                                            aGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAg                                                                                           >  2:872865/1‑140 (MQ=255)                                               gCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGa                                                                                        >  2:177527/1‑140 (MQ=255)                                                    cGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAg                                                                                   <  1:1119093/140‑1 (MQ=255)                                                       cGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTg                                                                                <  1:872865/140‑1 (MQ=255)                                                       cGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTg                                                                                <  1:177527/140‑1 (MQ=255)                                                       cGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTg                                                                                <  2:246074/140‑1 (MQ=255)                                                            cTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTggg                                                                           <  2:1059315/140‑1 (MQ=255)                                                            cTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTggg                                                                           <  1:584977/140‑1 (MQ=255)                                                                ttCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGggcggc                                                                       <  2:874006/140‑1 (MQ=255)                                                                ttCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGggcggc                                                                       <  2:723490/140‑1 (MQ=255)                                                                  cGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCAt                                                                     <  1:1048831/140‑1 (MQ=255)                                                                         cgcgAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTgg                                                                            >  2:363133/1‑126 (MQ=255)                                                                         cgcgAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTgg                                                                            <  1:363133/126‑1 (MQ=255)                                                                         cgcgAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTAt                                                              >  1:25692/1‑140 (MQ=255)                                                                                        ggCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAg                                               >  1:859832/1‑140 (MQ=255)                                                                                        ggCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAg                                               >  1:227675/1‑140 (MQ=255)                                                                                              ccGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCcg                                         >  2:773924/1‑140 (MQ=255)                                                                                                   gCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCt                                    >  1:829670/1‑140 (MQ=255)                                                                                                        agaagCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTAcc                               >  2:680940/1‑140 (MQ=255)                                                                                                            gCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGga                           <  2:829670/140‑1 (MQ=255)                                                                                                            gCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGga                           >  1:487908/1‑140 (MQ=255)                                                                                                            gCCCTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGga                           >  1:745262/1‑140 (MQ=255)                                                                                                              ccTGTGGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGgaga                         <  2:859832/140‑1 (MQ=255)                                                                                                                 gtgGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCCTTACCGGGAGATGc                      >  2:711307/1‑140 (MQ=255)                                                                                                                  tgGGCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGcc                     >  2:432318/1‑140 (MQ=255)                                                                                                                   gggCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCg                    <  2:487908/140‑1 (MQ=255)                                                                                                                   gggCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCg                    <  2:285150/140‑1 (MQ=255)                                                                                                                    ggCGCGTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCgg                   >  2:539052/1‑140 (MQ=255)                                                                                                                      cgcgTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTa                 <  1:432318/140‑1 (MQ=255)                                                                                                                        cgTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTaaa               <  2:8443/140‑1 (MQ=255)                                                                                                                        cgTTTATTTACGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTaaa               >  2:988996/1‑140 (MQ=255)                                                                                                                          tttatttaCGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACa             >  1:793325/1‑140 (MQ=255)                                                                                                                          tttatttaCGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACa             >  1:226225/1‑140 (MQ=255)                                                                                                                          tttatttaCGCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACa             <  2:1086604/140‑1 (MQ=255)                                                                                                                                   gCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACa             <  2:472670/131‑1 (MQ=255)                                                                                                                                   gCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACa             >  1:472670/1‑131 (MQ=255)                                                                                                                                   gCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTggg    >  2:441000/1‑140 (MQ=255)                                                                                                                                   gCCTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTggg    <  1:680940/140‑1 (MQ=255)                                                                                                                                     cTGCAGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGaa  >  1:614278/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        CCCTTATGCGTCATGTCTTAAGTCGCATGGCACTTCAGCTTGAAGGGCAAACGGCGTTGCTGTTTCGTCTTGCGCGAGCGTGGGACCGGCGTGCCGATGCCAAAGAAGCCCTGTGGGCGCGTTTATTTACGCCTGCGGCAAAATTTGTGATCTGCAAAAGAGGTATGCCGTTTGTGGCCGAAGCGATGGAGGTGCTGGGCGGCATTGGTTATTGCGAGGAGAGTGAGCTGCCGCGGCTTTACCGGGAGATGCCGGTAAACAGTATTTGGGAA  >  NC_000913/4415278‑4415549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑