Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	828,537	G→A	L149L (CTG→TTG)	ybhF ←	putative ABC exporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	828,537	0	G	A	100.0%	178.5 / NA	52	L149L (CTG→TTG)	ybhF	putative ABC exporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/23);  total (29/23)

ACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTAC  >  NC_000913/828400‑828673                                                                                                                                          |                                                                                                                                         aCTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAAc                                                                                                                                        >  1:577415/1‑140 (MQ=255)       gagGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGtt                                                                                                                                  >  2:1023981/1‑140 (MQ=255)          gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGttttt                                                                                                                               >  1:1058461/1‑140 (MQ=255)          gATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGttttt                                                                                                                               >  2:744823/1‑140 (MQ=255)             tAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGtt                                                                                                                            >  1:595704/1‑140 (MQ=255)                cATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCa                                                                                                                         <  1:1023981/140‑1 (MQ=255)                  tCCCTGCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATc                                                                                                                       <  2:429809/140‑1 (MQ=255)                   cccTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATcc                                                                                                                      <  2:412064/140‑1 (MQ=255)                                 gCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCACCGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTg                                                                                                        <  2:166322/140‑1 (MQ=255)                                  cTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGc                                                                                                       >  1:760750/1‑140 (MQ=255)                                   tCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGcc                                                                                                      <  1:989283/140‑1 (MQ=255)                                       gCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGcc                                                                                                  >  2:1008894/1‑140 (MQ=255)                                             tCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCgc                                                                                            >  1:208906/1‑139 (MQ=255)                                             tCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGt                                                                                            >  2:911645/1‑140 (MQ=255)                                              cTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTc                                                                                           <  1:911645/140‑1 (MQ=255)                                                     aGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTaaa                                                                                    <  1:158368/140‑1 (MQ=255)                                                         cgcgCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGc                                                                                >  1:180845/1‑140 (MQ=255)                                                          gcgcCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGcc                                                                               <  1:744823/140‑1 (MQ=255)                                                               gTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAaga                                                                          <  2:386133/140‑1 (MQ=255)                                                               gTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAaga                                                                          >  1:461195/1‑140 (MQ=255)                                                                 gagaTAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAgaga                                                                        >  2:880050/1‑140 (MQ=255)                                                                          tCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTc                                                               >  2:435386/1‑140 (MQ=255)                                                                           cAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTcc                                                              >  2:693595/1‑140 (MQ=255)                                                                                   acgccgGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCg                                                      >  1:27119/1‑140 (MQ=255)                                                                                          gTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCaaaa                                               <  1:880050/140‑1 (MQ=255)                                                                                              aTCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGc                                                       <  1:466788/128‑1 (MQ=255)                                                                                              aTCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGc                                                       >  2:466788/1‑128 (MQ=255)                                                                                              aTCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGttt                                           <  1:1008894/140‑1 (MQ=255)                                                                                               tCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTg                                          <  1:808777/140‑1 (MQ=255)                                                                                                        aaCACTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGc                                 <  1:962794/140‑1 (MQ=255)                                                                                                         acacTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGcc                                >  2:276440/1‑140 (MQ=255)                                                                                                           acTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTc                              <  1:312066/140‑1 (MQ=255)                                                                                                           acTTTCGGTTCGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTc                              <  2:595704/140‑1 (MQ=255)                                                                                                                 ggTTCGCCCACCAGGGTACAGGCCAAACCGACTTTTTGTTTCATCCCACCGTAGAGCTTGCCCGCCAGGCGTGCGGTAAACGGCCCAAGGGACATAAACTCCAGCAGGCGAGCAAAATTTTGCTTACGTGCCTCGCCgct                        >  2:1033476/1‑138 (MQ=255)                                                                                                                     cGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTc                              >  2:207042/1‑130 (MQ=255)                                                                                                                     cGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTc                              <  1:207042/130‑1 (MQ=255)                                                                                                                     cGCCCACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACg                    >  2:253630/1‑140 (MQ=255)                                                                                                                       cccACCAGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCt                  >  1:19179/1‑140 (MQ=255)                                                                                                                             aGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCaa            >  1:84039/1‑140 (MQ=255)                                                                                                                             aGGGTACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCaa            >  2:682711/1‑140 (MQ=255)                                                                                                                                 tACAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATcc        >  1:376278/1‑140 (MQ=255)                                                                                                                                  aCAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCcg       >  1:1001320/1‑140 (MQ=255)                                                                                                                                  aCAGGCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCcg       <  1:435386/140‑1 (MQ=255)                                                                                                                                     ggCCAAACCGAGTTTTTGTTTCTTCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGt    <  1:1033476/140‑1 (MQ=255)                                                                                                                                     ggCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGt    <  2:760750/140‑1 (MQ=255)                                                                                                                                      gCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACg                    >  1:26721/1‑123 (MQ=255)                                                                                                                                      gCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACg                    <  2:26721/123‑1 (MQ=255)                                                                                                                                      gCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTa   >  2:400341/1‑140 (MQ=255)                                                                                                                                      gCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTa   >  1:3648/1‑140 (MQ=255)                                                                                                                                      gCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTa   <  2:385242/140‑1 (MQ=255)                                                                                                                                      gCCAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTa   >  1:466816/1‑140 (MQ=255)                                                                                                                                       ccAAACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTAc  <  1:276440/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         ACTCCAGAGGATTAACATCCCTTCGCCCGCCAGCTCATGCACCATCTGCCACAGTTCGCGCCGTGAGATAGGGTCAACGCCGACGCCGGGTTCATCGAGCAGCAACACTTTCGGTTCGCCCACCAGGGTACAGGCCAGACCGAGTTTTTGTTTCATCCCACCGGAGAGCTTGCCCGCCAGGCGTCCGGTAAACGGCCCAAGAGACGTAAACTCCAGCAGGCGAGCAAAAGTTTGCTTACGTGCCTCGCCGGTGACGCTGCGCAAATCCGCGTAC  >  NC_000913/828400‑828673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑