Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913445,5580AC43.5% 2.0 / 22.1 23V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (9/4);  new base C (0/10);  total (9/14)
Fisher's exact test for biased strand distribution p-value = 1.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTT  >  NC_000913/445425‑445682
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aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                        <  2:489015/140‑1 (MQ=255)
aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                        <  2:255388/140‑1 (MQ=255)
             gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                           <  1:138537/140‑1 (MQ=255)
                     ggCGGCAATGTTCGCAGTGATTTCAATGCGCAAACTGTTGACATACGGGGGTTCTTTCAGGGAACGGGCGACTTTCATCCACACGGCGGCCAGCATTGCGCCAGCGAGAAATTCCCCCAGCCCGTAAAACATGCCGTTaa                                                                                                   <  2:194831/139‑1 (MQ=255)
                                                 cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                       <  1:681857/140‑1 (MQ=255)
                                                             aCATACGGCGGTTCTTTTATTGTACTGGCGACTGTCAGCCCCACCGCGGGCCGCATTGCGCCCGCGGGAAAAAACCCCTGACCGTCAAAAATGCCGTTAATCCAGCCGCCCCGCGAAACCCCCAACGCCCCCCCCAGaaa                                                           >  2:423595/1‑140 (MQ=255)
                                                                  gggcggTTCTTTCAGGGTACTGGCGACTGTCAGCCACACGGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                      <  2:713218/139‑1 (MQ=255)
                                                                  cggcggTTCTTTTATGGGACTTGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCCGCGGGAAAAACCCCCCGACCCTCAAACATGCCCTTAATCCAGCCCCCCCGCGAAACCCCCAACGCCCCCCCAAGaaaaaggc                                                      >  2:644815/1‑135 (MQ=255)
                                                                      ggTTCTTTCATGGTCCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                  <  2:251428/140‑1 (MQ=255)
                                                                       gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTTCGCCAGCGAGAAATACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAAACGCCAATCGCCACGCCAAGAAActggctgggg                                                 >  2:448042/1‑138 (MQ=255)
                                                                        ttCTTTCATGGTACTGGCGACTGTCAGCCCCACCGCGGGCCGCATTGCGCCAGCGGGAAAAAACCCCTGACCCGCAAACAAGCCGTTTATCCACCCCCCCCCCGAACCGCCAAACCCCACCCCAAGAAAAAGGCcggtgg                                                >  2:540324/1‑140 (MQ=255)
                                                                                  gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                      >  2:847490/1‑140 (MQ=255)
                                                                                  gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                      >  2:841943/1‑140 (MQ=255)
                                                                                    aCTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCa                                    >  1:823974/1‑140 (MQ=255)
                                                                                     gagacagcTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCTAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                   <  2:574855/133‑1 (MQ=255)
                                                                                      tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                  >  1:102487/1‑140 (MQ=255)
                                                                                          gccTGTCAGCCCCACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                              <  2:124979/138‑1 (MQ=255)
                                                                                           ccTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                             <  1:139387/139‑1 (MQ=255)
                                                                                             tgccacccACACTGCGCCCACCTTTGCGCCGGCGAAAAATCCCCCCGGCCCGCCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                           <  2:823974/134‑1 (MQ=255)
                                                                                             tGTCAGCCACACTGCGGCTAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                           >  2:550330/1‑140 (MQ=255)
                                                                                                   ccACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGt                     >  2:778546/1‑140 (MQ=255)
                                                                                                    cacaCTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTa                    <  1:448042/140‑1 (MQ=255)
                                                                                                      cacTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAc                  <  1:540324/140‑1 (MQ=255)
                                                                                                       acTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCTAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCttctgtctc                 >  1:574855/1‑133 (MQ=255)
                                                                                                           cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCATCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct             >  1:739441/1‑140 (MQ=255)
                                                                                                             gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg           >  1:645196/1‑140 (MQ=255)
                                                                                                             gCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg           <  2:328778/140‑1 (MQ=255)
                                                                                                             cccAGCTTGGCGCAAGGGAGAAACCCCCCCGGCCCTCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg           <  2:739441/139‑1 (MQ=255)
                                                                                                                aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa        <  1:847490/140‑1 (MQ=255)
                                                                                                                aGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa        <  1:841943/140‑1 (MQ=255)
                                                                                                                    ttGCGCAAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct    <  1:722707/140‑1 (MQ=255)
                                                                                                                    ttGCCCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct    <  1:733343/140‑1 (MQ=255)
                                                                                                                     ttcgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt   <  1:835503/138‑1 (MQ=255)
                                                                                                                      gcgcCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt  <  1:778546/140‑1 (MQ=255)
                                                                                                                      gcgcCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt  <  1:644815/140‑1 (MQ=255)
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ACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTT  >  NC_000913/445425‑445682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑