Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2070TC34.6% 31.8 / 10.0 26S215G (AGT→GGT) ‡rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (15/2);  new base C (0/9);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 1.76e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556074‑3556343
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gCACTGCCGCGCTTTTCCCCGACGACAAAAAGGCGTTCGGTGATATTTTCCCTTTCGACTTCAAGCGAGACGGTATTCCCCGGCCCCTGGCCGCGCGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAACCCCCCCGc                                                                                                                                    <  2:23603/140‑1 (MQ=255)
        gcgtTTTTCCCCGACGAAAAAAAAGCGTTGGGTGATATTTTCACTTTCGATTTCAAGCGAGACGTTATTCCCCGGCCCCGGGCCGCGGGGCAGTTTATGAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAg                                                                                                                            <  2:400906/140‑1 (MQ=255)
           cTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGa                                                                                                                         >  1:98354/1‑140 (MQ=255)
                  ccGACAAAAAAAAAGCGTTGGGTGATTTTTTCTTTTCCGACTTAAAGCGAGACGGTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATGAATATTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:836750/140‑1 (MQ=255)
                    aacaaaaaaaagGTGTTCGGTAATTTTTTCATTTCCGACTTCAAGCGAGCCGTTTTTCCCCGGCCCTGGGCCGCGGGGGAGTTTATAAATTTTTTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                <  2:196624/134‑1 (MQ=255)
                        acAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGc                                                                                                            >  2:56471/1‑140 (MQ=255)
                              aggCGTTCGGTATTTTTTTCCCTTTCGCTTAAAAGGGGGACGTTATTCCCCGCCCCCTGGCCGCGCGGCAGTTTATAAATATTTTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATg                                                                                                      <  2:803073/138‑1 (MQ=255)
                               aGCGTTCGGTGATTTTTTCACTTTCGACTAAAAGCGAGACGGTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATGAATATTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATgg                                                                                                     <  2:497776/140‑1 (MQ=255)
                                   ttCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGcgcg                                                                                                 >  1:740979/1‑140 (MQ=255)
                                    tCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCgg                                                                                                >  2:628929/1‑140 (MQ=255)
                                           ttttttCTCTTTCCAGTCAAGGCGAGACGTTATCCCCGGGCCCTTGGTCGCGCGGCTGTTTATGAATATTTTGTGCAGGGGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                         <  2:98354/138‑1 (MQ=255)
                                           tatTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                         >  1:242868/1‑140 (MQ=255)
                                                tCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTaa                                                                                    >  1:1848/1‑140 (MQ=255)
                                                               aGCGAGCCGTTTTTCCCCGGCCCCTGGCCGCGGGGCAGTTTATGAATTTTCTTTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACg                                                                     <  2:347122/140‑1 (MQ=255)
                                                                  gagcCGGTATTCCCCGCCCCCTGGTCGCGCGGCAGTTTATAAATTTTTTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                  <  2:1848/140‑1 (MQ=255)
                                                                    gaCGGTATCACCCGCCCCCTGGTCGCGCGGCAGGTTATGAATTTTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCt                                                                <  2:59216/140‑1 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  1:13830/1‑140 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  1:574374/1‑140 (MQ=255)
                                                                                   ccccTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgc                                                 >  1:455210/1‑140 (MQ=255)
                                                                                        ggccgcgggGCAGTTTATAAATATTCTTTTCAGGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                            <  1:628929/137‑1 (MQ=255)
                                                                                        ggTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                            >  1:230835/1‑140 (MQ=255)
                                                                                             cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  2:803006/1‑140 (MQ=255)
                                                                                                  cAGTTTATGAATATTCTGTCCAGGCAGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  1:411319/140‑1 (MQ=255)
                                                                                                  cAGGTTATAAATATTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:13830/140‑1 (MQ=255)
                                                                                                               ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  1:790090/1‑140 (MQ=255)
                                                                                                               ttCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  2:581628/1‑140 (MQ=255)
                                                                                                                tCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  1:542234/1‑140 (MQ=255)
                                                                                                                   gTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGcc                 >  2:795297/1‑140 (MQ=255)
                                                                                                                    ttCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  1:795297/140‑1 (MQ=255)
                                                                                                                      cggggaGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg              <  2:230835/135‑1 (MQ=255)
                                                                                                                                  aaCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  <  2:542234/140‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                        
GCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556074‑3556343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑