Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,865,743	T→G	I244L (ATC→CTC)	mipA ←	scaffolding protein that interacts with murein polymerase and murein hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,865,743	0	T	G	100.0%	124.1 / NA	32	I244L (ATC→CTC)	mipA	scaffolding protein that interacts with murein polymerase and murein hydrolase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (18/14);  total (18/14)

AAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGC  >  NC_000913/1865613‑1865873                                                                                                                                   |                                                                                                                                   aaaTTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGa                                                                                                                           >  2:650517/1‑140 (MQ=255)         tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGc                                                                                                                   <  1:1061/140‑1 (MQ=255)         tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGc                                                                                                                   <  2:503816/140‑1 (MQ=255)         tCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGc                                                                                                                   <  1:409285/140‑1 (MQ=255)                 tgttgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAg                                                                                                           >  2:263872/1‑140 (MQ=255)                    tgCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGAt                                                                                                        <  1:31830/140‑1 (MQ=255)                                tccCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATc                                                                                            >  2:642879/1‑140 (MQ=255)                                   cAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCggg                                                                                         <  1:30531/140‑1 (MQ=255)                                         ttGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCa                                                                                   <  1:642879/140‑1 (MQ=255)                                            caccacCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTa                                                                                >  2:863494/1‑140 (MQ=255)                                            caccacCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTa                                                                                >  1:398807/1‑140 (MQ=255)                                                 ccGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtc                                                                           >  2:37818/1‑140 (MQ=255)                                                    tAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatc                                                                        >  1:105142/1‑140 (MQ=255)                                                     aaTGCAAAACAGGGCGTGACGCCCTGTTTTTATACACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTtcatca                                                                       <  1:603224/140‑1 (MQ=255)                                                        gCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagac                                                                    >  1:818655/1‑140 (MQ=255)                                                            aaCAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCagacagac                                                                >  1:396477/1‑140 (MQ=255)                                                              cAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACgg                                                              >  1:482247/1‑140 (MQ=255)                                                                   cGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTa                                                         <  1:37818/140‑1 (MQ=255)                                                                   cGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTa                                                         <  2:482247/140‑1 (MQ=255)                                                                        cGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTg                                                    <  2:471425/140‑1 (MQ=255)                                                                            cTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                                                                 >  1:391067/1‑123 (MQ=255)                                                                            cTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATcagacaga                                                                 <  2:391067/123‑1 (MQ=255)                                                                            cTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGt                                                <  2:818655/140‑1 (MQ=255)                                                                               tttttATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTAcc                                             >  1:571872/1‑140 (MQ=255)                                                                                         caAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTc                                   >  1:214821/1‑140 (MQ=255)                                                                                                 ccTGGAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGcc                           >  2:69678/1‑140 (MQ=255)                                                                                                    ggAAAGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAg                        <  2:369460/140‑1 (MQ=255)                                                                                                       aaGATGCATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGaa                     >  1:108000/1‑140 (MQ=255)                                                                                                             cATTATCAGAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTa               >  2:716552/1‑140 (MQ=255)                                                                                                                     gAATTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGgcgc       >  2:445653/1‑140 (MQ=255)                                                                                                                      aaTTTGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCt      <  1:263872/140‑1 (MQ=255)                                                                                                                          tGTAGGTGAGCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGc  >  1:94536/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                   AAATTGAATCATTTTTTGTTGCATTGTTTCCTCCCAGGTATTGCACCACCGTAATGCAAAACAGGGCGTGACGCCCTGTTTTTATGCACAAAATGCCCTGGAAAGATGCATTATCAGAATTTGTAGGTGATCCCGGTAGAAATCAGGCCAGTCCAGGATTTATCCACCATCGGGCTGTCAGTAACTTCATCAGACAGACGGGTGTAGCGTGCGGTACCGTAAACACTCCAGTCGCCGAGGAAGTTGTAGCTGGCGCTCAGC  >  NC_000913/1865613‑1865873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑