Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,234,161	T→A	D43E (GAT→GAA)	preT →	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,234,161	0	T	A	100.0%	130.7 / NA	42	D43E (GAT→GAA)	preT	NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/23);  total (19/23)

GATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTAC  >  NC_000913/2234027‑2234295                                                                                                                                       |                                                                                                                                       gATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCggg                                                                                                                                   >  1:583228/1‑140 (MQ=255) gATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCggg                                                                                                                                   <  1:436173/140‑1 (MQ=255)  aTCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCgggg                                                                                                                                  >  1:157248/1‑140 (MQ=255)      caTGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAAt                                                                                                                              >  1:804279/1‑140 (MQ=255)         gCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTa                                                                                                                           <  2:784858/140‑1 (MQ=255)            gCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTc                                                                                                                        <  1:453566/140‑1 (MQ=255)             caacaaAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCg                                                                                                                       >  2:689015/1‑140 (MQ=255)              aacaaAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCATGCCCGGCACAGACCGAACCGGGGAAATTTATTCGc                                                                                                                      <  2:742009/140‑1 (MQ=255)               acaaAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCt                                                                                                                     <  1:253674/140‑1 (MQ=255)                             aTGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTc                                                                                                         >  2:218799/1‑138 (MQ=255)                             aTGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTc                                                                                                         <  1:218799/138‑1 (MQ=255)                                aaCTCACTCCGGCTTTTACGTATTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAAt                                                                                                    >  1:676126/1‑140 (MQ=255)                                      ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTaaa                                                                                              >  2:36556/1‑140 (MQ=255)                                      ctcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTaaa                                                                                              >  2:539681/1‑140 (MQ=255)                                       tcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAg                                                                                             >  1:581367/1‑140 (MQ=255)                                       tcCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAg                                                                                             >  1:651144/1‑140 (MQ=255)                                          ggCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGgcg                                                                                          <  2:157248/140‑1 (MQ=255)                                                 aCGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCga                                                                                   <  1:539681/140‑1 (MQ=255)                                                      tttACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACaa                                                                              <  2:731017/140‑1 (MQ=255)                                                             gCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGa                                                                       <  2:581367/140‑1 (MQ=255)                                                                  tAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAaata                                                                  <  2:583228/140‑1 (MQ=255)                                                                       aagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGcc                                                             <  1:689015/140‑1 (MQ=255)                                                                       aagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGcc                                                             >  2:193893/1‑140 (MQ=255)                                                                       aagCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAA‑TTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGccc                                                            >  2:309263/1‑140 (MQ=255)                                                                         gCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCt                                                           <  1:642707/140‑1 (MQ=255)                                                                         gCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCt                                                           <  2:804279/140‑1 (MQ=255)                                                                         gCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAA‑TTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTc                                                          <  1:309263/140‑1 (MQ=255)                                                                              tcGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTg                                                      <  2:676126/140‑1 (MQ=255)                                                                              tcGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTAAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCt                                                           >  2:546490/1‑135 (MQ=255)                                                                              tcGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTAAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCt                                                           <  1:546490/135‑1 (MQ=255)                                                                                  tGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGt                                                  <  1:439726/140‑1 (MQ=255)                                                                                    tttATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGttt                                                <  2:453978/140‑1 (MQ=255)                                                                                              cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCaga                                        <  2:175255/138‑1 (MQ=255)                                                                                              cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCaga                                        >  1:175255/1‑138 (MQ=255)                                                                                              cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAgt                                      >  2:814784/1‑140 (MQ=255)                                                                                              cACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAgt                                      >  1:420457/1‑140 (MQ=255)                                                                                                     cTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCg                               >  1:722487/1‑140 (MQ=255)                                                                                                                    cTTGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGc                >  1:579365/1‑140 (MQ=255)                                                                                                                      tGCCCGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCa              >  2:631080/1‑140 (MQ=255)                                                                                                                        cccGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaaa            <  2:750860/140‑1 (MQ=255)                                                                                                                        cccGGCACAGACCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCaaa            <  1:36556/140‑1 (MQ=255)                                                                                                                                  aCCGAACCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTAc  <  1:814784/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GATCACATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGTCTTTACTGGCGATTAAAGAAGCCTCTCGCTGTTTATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTCGTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAGAAATTATGCCAAAGCGGTTGTAC  >  NC_000913/2234027‑2234295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑