Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,875,207 T→C T124A (ACT→GCT)  nimR ← DNA‑binding transcriptional repressor NimR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,875,2070TC100.0% 158.5 / NA 42T124A (ACT→GCT) nimRDNA‑binding transcriptional repressor NimR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/19);  total (23/19)

TTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGTCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCATTAGCTGTTACTTGA  >  NC_000913/1875070‑1875343
                                                                                                                                         |                                                                                                                                        
tttCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAgccg                                                                                                                                        <  1:77336/140‑1 (MQ=255)
 ttCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAgccgt                                                                                                                                       >  2:371817/1‑140 (MQ=255)
                    tCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGtt                                                                                                                    >  2:825205/1‑140 (MQ=255)
                     cGTGCGGATTTTAGGATTAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTa                                                                                                                   >  1:240721/1‑140 (MQ=255)
                     cGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTa                                                                                                                   >  2:783409/1‑140 (MQ=255)
                                tAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTcc                                                                                                        <  2:403423/140‑1 (MQ=255)
                                   gATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCgg                                                                                                     >  2:784953/1‑140 (MQ=255)
                                      gagaAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCAt                                                                                                  >  2:740850/1‑140 (MQ=255)
                                      gagaAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGAGGGTCACTGCCACAGCACGCTGAGGCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCAt                                                                                                  >  2:966230/1‑140 (MQ=255)
                                       agaagaAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATa                                                                                                 <  2:742556/140‑1 (MQ=255)
                                        gaagaaACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATaa                                                                                                >  1:379419/1‑140 (MQ=255)
                                        gaagaaACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATa                                                                                                 <  2:409271/139‑1 (MQ=255)
                                        gaagaaACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATa                                                                                                 >  1:409271/1‑139 (MQ=255)
                                          agaaACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAgg                                                                                              <  1:139446/140‑1 (MQ=255)
                                            aaaCGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGc                                                                                            >  2:257411/1‑140 (MQ=255)
                                              aCGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGa                                                                                          <  2:388528/140‑1 (MQ=255)
                                                 ggCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGatat                                                                                       >  1:23593/1‑140 (MQ=255)
                                                   cAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATAttt                                                                                     >  1:372985/1‑140 (MQ=255)
                                                      gTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCa                                                                                  <  1:783409/140‑1 (MQ=255)
                                                           gCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCGCAACACGCTGAGCCGTCGTCCTGTTTGCTACGGGTAAAATTAATTTTCGGCATAACGGCGATATTTTCATtgtg                                                                             >  2:331800/1‑140 (MQ=255)
                                                                 gttgtGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACAt                                                                       >  1:446886/1‑140 (MQ=255)
                                                                     tgGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACAtgttg                                                                   <  1:331800/140‑1 (MQ=255)
                                                                      ggCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACAtgttgt                                                                  >  2:560870/1‑140 (MQ=255)
                                                                            tgAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCAt                                                            >  2:64412/1‑140 (MQ=255)
                                                                                 gAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCa                                                       <  2:240721/140‑1 (MQ=255)
                                                                                 gAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCa                                                       <  2:372985/140‑1 (MQ=255)
                                                                                 gAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCa                                                       <  1:784953/140‑1 (MQ=255)
                                                                                   gTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACt                                                     >  1:302809/1‑140 (MQ=255)
                                                                                     tcatcaAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTgc                                                   >  1:748694/1‑140 (MQ=255)
                                                                                      catcaAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTgcg                                                  >  2:814986/1‑140 (MQ=255)
                                                                                      catcaAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTgcg                                                  <  2:379419/140‑1 (MQ=255)
                                                                                      catcaAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTgcg                                                  <  1:64412/140‑1 (MQ=255)
                                                                                            aCAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGt                                            <  2:136547/140‑1 (MQ=255)
                                                                                              aGGACCTGAATTAATCTGCGGGTCATTGGCTCTGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTc                                          >  2:805705/1‑140 (MQ=255)
                                                                                                          aaTCTGCGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATaaacagaaa                              >  1:276442/1‑140 (MQ=255)
                                                                                                                cGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACagag                        >  2:614108/1‑140 (MQ=255)
                                                                                                                cGGGTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACagag                        >  1:774414/1‑140 (MQ=255)
                                                                                                                 gggTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGt                       <  2:748694/140‑1 (MQ=255)
                                                                                                                   gTCATTGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTc                     <  1:740850/140‑1 (MQ=255)
                                                                                                                       ttGGCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCa                 <  1:814986/140‑1 (MQ=255)
                                                                                                                         ggCTCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCAtt               <  2:774414/140‑1 (MQ=255)
                                                                                                                            tCAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCATTAGc            <  1:560870/140‑1 (MQ=255)
                                                                                                                             cAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCATTAGCt           >  1:439490/1‑140 (MQ=255)
                                                                                                                             cAGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCATTAGCt           >  1:368591/1‑140 (MQ=255)
                                                                                                                              aGCACGCTGAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCATTAGCTg          >  1:948401/1‑140 (MQ=255)
                                                                                                                                      gAGCCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCATTAGCTGTTACTTGa  <  2:23593/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
TTTCGCCATCATCTCCACCATCGTGCGGATTTTAGGATGAGAAGAAACGGGCAGGTGCAGCTGTTGTTGTGGCTGCTGAGGGAGTTCATCAAACAGGACCTGAATTAATCTGCGGGTCATTGGCTCAGCACGCTGAGTCGTCGTCCTGTTTGCCAGGGTTAAAATTAATTCCCGGCATAAGGGCGATATTTTCAATGTGCAACATGTTGTCGGCATTGTCACTGCGCTGGGTTCGATAAACAGAAAACAGAGTTCAGCATTAGCTGTTACTTGA  >  NC_000913/1875070‑1875343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑