Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,394,973	A→G	V317A (GTA→GCA)	ynaI ←	small conductance mechanosensitive channel YnaI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,394,973	0	A	G	100.0%	250.2 / NA	64	V317A (GTA→GCA)	ynaI	small conductance mechanosensitive channel YnaI
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (28/36);  total (28/36)

ATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGT  >  NC_000913/1394840‑1395106                                                                                                                                      |                                                                                                                                      atgtgatgGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAatat                                                                                                                                 >  1:234157/1‑140 (MQ=255)         ggAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATc                                                                                                                         <  1:202735/140‑1 (MQ=255)           aaCTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCtt                                                                                                                       >  2:128396/1‑140 (MQ=255)             cTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCa                                                                                                                     >  2:88441/1‑140 (MQ=255)                   ttATCCCTGTGAATAGCGCCAGTGATGTTTGATTTAGCGACCCTGTTCTGACGGTGTAACGTTATCCATATCTGGCGTCTGGGTGGGGGAGGCTAACTCCGCACCGTGTGACTGAGCAATATCGATAATCTTCAAATaaa                                                                                                               >  2:374364/1‑140 (MQ=255)                          tgtgCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATGCAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTg                                                                                                        <  2:854697/140‑1 (MQ=255)                          tgtgCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTg                                                                                                        >  1:715812/1‑140 (MQ=255)                          tgtgCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTg                                                                                                        <  2:715812/140‑1 (MQ=255)                           gtgCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGc                                                                                                       >  2:491280/1‑140 (MQ=255)                            tgCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCt                                                                                                      <  1:530575/140‑1 (MQ=255)                            tgCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCt                                                                                                      >  2:784323/1‑140 (MQ=255)                                 aaCGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCCGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTgca                                                                                                 >  1:308673/1‑140 (MQ=255)                                   cGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTgcagc                                                                                               >  2:299626/1‑140 (MQ=255)                                     ccAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCaa                                                                                             <  1:299626/140‑1 (MQ=255)                                      cAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAg                                                                                            >  2:117462/1‑140 (MQ=255)                                      cAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAg                                                                                            >  1:468074/1‑140 (MQ=255)                                      cAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAg                                                                                            >  1:909644/1‑140 (MQ=255)                                          gATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCAt                                                                                        <  1:88441/140‑1 (MQ=255)                                          gATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCAt                                                                                        <  2:909644/140‑1 (MQ=255)                                          gATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCAt                                                                                        <  1:536863/140‑1 (MQ=255)                                          gATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCAt                                                                                        <  2:430430/140‑1 (MQ=255)                                           aTGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCAtt                                                                                       <  2:92893/140‑1 (MQ=255)                                           aTGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCAtt                                                                                       <  1:662060/140‑1 (MQ=255)                                               cTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGc                                                                                   <  1:784323/140‑1 (MQ=255)                                               cTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGc                                                                                   <  2:501966/140‑1 (MQ=255)                                                 gATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGccc                                                                                 <  1:491280/140‑1 (MQ=255)                                                     tAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATAc                                                                             >  1:945914/1‑140 (MQ=255)                                                      aGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATAcc                                                                            <  2:739564/140‑1 (MQ=255)                                                       gCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCg                                                                           >  1:194699/1‑140 (MQ=255)                                                        cGACCCTGTTCCGGCGGTGGAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGt                                                                          >  2:340924/1‑140 (MQ=255)                                                            ccTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGtt                                                                      >  1:425947/1‑140 (MQ=255)                                                                  ccGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTa                                                                <  2:993693/140‑1 (MQ=255)                                                                     gcggTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAg                                                             <  1:340924/140‑1 (MQ=255)                                                                        gtgtAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCaa                                                          <  1:531309/140‑1 (MQ=255)                                                                            aaTGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATaaa                                                      <  1:353207/140‑1 (MQ=255)                                                                                 tATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCata                                                 <  1:235939/140‑1 (MQ=255)                                                                                 tATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCata                                                 <  2:644536/140‑1 (MQ=255)                                                                                 tATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCata                                                 <  2:240085/140‑1 (MQ=255)                                                                                 tATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCata                                                 <  2:468074/140‑1 (MQ=255)                                                                                   tCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCataat                                               >  1:746180/1‑140 (MQ=255)                                                                                   tCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCa                                                   <  1:210480/136‑1 (MQ=255)                                                                                   tCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCa                                                   >  2:210480/1‑136 (MQ=255)                                                                                     cATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAatat                                             <  2:308673/140‑1 (MQ=255)                                                                                      atatACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATAtt                                            <  2:627452/140‑1 (MQ=255)                                                                                          aCAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAAt                                        <  1:693634/140‑1 (MQ=255)                                                                                          aCAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAAt                                        <  1:167942/140‑1 (MQ=255)                                                                                              cGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAag                                    >  1:593733/1‑140 (MQ=255)                                                                                              cGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAag                                    <  2:425947/140‑1 (MQ=255)                                                                                              cGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAag                                    >  1:587525/1‑140 (MQ=255)                                                                                              cGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAag                                    >  2:867444/1‑140 (MQ=255)                                                                                              cGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAag                                    >  1:826322/1‑140 (MQ=255)                                                                                               gTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTCGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAaga                                   <  1:886887/140‑1 (MQ=255)                                                                                                 ctggctggGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCTAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGt                                 <  2:587525/140‑1 (MQ=255)                                                                                                  tggctggGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTc                                >  2:964166/1‑140 (MQ=255)                                                                                                     ctggGGAAGGCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGc                             >  2:17700/1‑140 (MQ=255)                                                                                                             ggCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGt                     <  1:17700/140‑1 (MQ=255)                                                                                                              gCAAAGTCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGtt                    >  1:337169/1‑140 (MQ=255)                                                                                                                    tCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATa              <  1:128396/140‑1 (MQ=255)                                                                                                                    tCCGCGCCGTGTGACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATa              <  2:746180/140‑1 (MQ=255)                                                                                                                             tgtgACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTaa     >  1:415493/1‑140 (MQ=255)                                                                                                                              gtgACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAg    >  2:765085/1‑140 (MQ=255)                                                                                                                              gtgACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAg    >  2:408263/1‑140 (MQ=255)                                                                                                                              gtgACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAg    >  1:791869/1‑140 (MQ=255)                                                                                                                                gACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGt  <  2:194699/140‑1 (MQ=255)                                                                                                                                gACTGTGCAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGt  <  1:964166/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      ATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAGCCATTCAGCCCATACCGTGGTTTTGGTAAAGCAATAAACCATAATATTCAATGAAGAGTCAGCAAACTGGTTGAAATAAACCAGTAAGGT  >  NC_000913/1394840‑1395106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑