Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT40.3% 63.6 / 43.2 62S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (6/31);  new base T (25/0);  total (31/31)
Fisher's exact test for biased strand distribution p-value = 9.99e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604205‑4604481
                                                                                                                                           |                                                                                                                                         
aaaTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttc                                                                                                                                           <  2:972455/140‑1 (MQ=255)
  aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttt                                                                                                                                         >  2:300401/1‑140 (MQ=255)
    gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttttttt                                                                                                                                       >  2:864851/1‑140 (MQ=255)
    gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctttt                                                                                                                                       >  2:454449/1‑140 (MQ=255)
     cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttttt                                                                                                                                      <  1:246016/140‑1 (MQ=255)
        cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTg                                                                                                                                   >  1:437615/1‑140 (MQ=255)
         aTGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGc                                                                                                                                  <  2:437615/140‑1 (MQ=255)
           gacaggtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCa                                                                                                                                <  1:883958/135‑1 (MQ=255)
            agtagtATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCAt                                                                                                                               <  1:482538/140‑1 (MQ=255)
                gtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                           <  1:620451/140‑1 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAgg                                                                                                                        >  2:908542/1‑139 (MQ=255)
                   tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                        <  1:454449/140‑1 (MQ=255)
                   caggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                        <  1:815712/138‑1 (MQ=255)
                      gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAagagag                                                                                                                     >  2:482538/1‑140 (MQ=255)
                       tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAgg                                                                                                                    >  1:511627/1‑139 (MQ=255)
                        aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAgcg                                                                                                                   <  1:635971/140‑1 (MQ=255)
                           gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGAAGGGAgctgtc                                                                                                                >  2:883958/1‑136 (MQ=255)
                                tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCTTGAGAAAAGAGCGCAGAgggg                                                                                                           >  2:222951/1‑140 (MQ=255)
                                tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAgg                                                                                                           >  1:511077/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGTTTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                          >  2:829794/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTTCATGAGAAGTGAGGGCAGgggggg                                                                                                          >  2:828581/1‑140 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                                                                          <  2:55803/140‑1 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAGTTTTTTTTTTTTTTGCATGAGAAGGGAGCGCagagagtg                                                                                                          >  2:815712/1‑138 (MQ=255)
                                 ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTTTTCCATTAGAAGGGAGGCCCGAGAggg                                                                                                          >  1:119325/1‑140 (MQ=255)
                                  tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGa                                                                                                         <  1:222951/140‑1 (MQ=255)
                                   aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAAGGAGCGGAGAGAggggt                                                                                                        >  2:512415/1‑138 (MQ=255)
                                   aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                        >  2:469969/1‑140 (MQ=255)
                                          aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGGTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                 <  2:356460/140‑1 (MQ=255)
                                          aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGATTAttttt                                                                                                 >  2:527522/1‑139 (MQ=255)
                                           aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                                                                                <  1:469969/140‑1 (MQ=255)
                                                   ggTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactga                                                                                        <  1:908542/140‑1 (MQ=255)
                                                      aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTTTCTTTGCTGACTGaaat                                                                                     >  2:860312/1‑138 (MQ=255)
                                                       aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCCTGAGAAGGGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAACTg                                                                                    >  1:497726/1‑140 (MQ=255)
                                                                  cTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGTCATGAGAAGTGAGCGCCGGGAGGGATTATTTTGCCTGACTGAACTGGCGGTTTAGtt                                                                         >  2:687089/1‑140 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAGGGGTTATTTTTCCTGGCTGAACTGGGGGTTAAGTTTTCGCGTAcc                                                               >  2:660705/1‑140 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                               <  1:305259/140‑1 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                               <  1:892882/140‑1 (MQ=255)
                                                                            ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc                                                               <  1:687089/140‑1 (MQ=255)
                                                                             taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTGCCTGACTGAAATGGCGGTTAAGTTTCCGCGTAccc                                                              >  2:1122326/1‑140 (MQ=255)
                                                                             taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                                                              <  1:860312/140‑1 (MQ=255)
                                                                             taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc                                                              <  1:1054830/140‑1 (MQ=255)
                                                                              aCCAGTCAGGAGTCAGTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCg                                                             <  1:1164325/140‑1 (MQ=255)
                                                                                 aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAAATTGCGATTTAATTTTCGCGTACCCcgcg                                                          >  2:810620/1‑140 (MQ=255)
                                                                                 aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTACCATGAGAAGTGAGCGCAGAGGGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAGTTTCCGCGTACCCgggg                                                          >  2:104151/1‑138 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGGGTATTTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCGGCGt                                                         >  2:198438/1‑140 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGCGCAGAGAGGGATTATTTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGGACCCGGCgg                                                         >  2:913951/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAAGTTCCGCGTACCCGGCgg                                                         >  2:602364/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGAAGCGCAGCGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTAAATTTCCGCGTACCCGGCgg                                                         >  2:717067/1‑139 (MQ=255)
                                                                                  gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATTTTGCCTGACTGAACTGGCGATTAAGTTTTCGCGTACCCGGGGt                                                         >  2:892882/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTTCCTGACTGAACTGGCGGTTTAGTTTTCGCGTACCCCGCGtt                                                        >  2:374170/1‑140 (MQ=255)
                                                                                   tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGGGATTAAGTTTCCGCGTACCCcgcggt                                                        >  2:973747/1‑138 (MQ=255)
                                                                                    cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGttt                                                       <  1:527522/140‑1 (MQ=255)
                                                                                       gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAGGGCCGGGGGGGGTTATCTTTCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCCGCGGTTAGt                                                    >  2:916960/1‑140 (MQ=255)
                                                                                           cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTAtt                                                <  1:300401/140‑1 (MQ=255)
                                                                                            aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:512415/140‑1 (MQ=255)
                                                                                              ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  1:864851/140‑1 (MQ=255)
                                                                                                 aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                          <  1:829794/140‑1 (MQ=255)
                                                                                                   gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  2:119325/140‑1 (MQ=255)
                                                                                                    aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:797611/140‑1 (MQ=255)
                                                                                                    aTGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgat                                       <  1:1122326/140‑1 (MQ=255)
                                                                                                     ggCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatg                                      <  1:353547/139‑1 (MQ=255)
                                                                                                      gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatga                                     <  1:1146139/140‑1 (MQ=255)
                                                                                                               cgGATCTCGTTTGCGGCGGTCAATTTTTTTTTTTTTTTCGCGGGAAGCCAGAGCAGGGGGGGGGTATTTTGCCTGACTGAAATGGCGATTAAGGTTTCCCGTACCCCGCGCTTTGTTATTGGGGGTGATGATATTTAAgg                            >  2:797611/1‑139 (MQ=255)
                                                                                                                 gATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGGTTTTTTTTCCTGACTGAACTGGCGGTTAAATTTTCGCGGACCCGGCGTTTTGTTTTTTCGGGTGATGATATTTAAGCTc                          >  2:353547/1‑140 (MQ=255)
                                                                                                                  aTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGGGGGGTTATCTTGCCTTACTGAACTGGCGGTTAAAGTTTCGCGTACCCGGCGGTTTGTTTTTGCGGGTGATGATATTTAAGCTCg                         >  2:662333/1‑140 (MQ=255)
                                                                                                                  aTCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTAAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                         <  1:717067/140‑1 (MQ=255)
                                                                                                                       gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                    <  1:814298/140‑1 (MQ=255)
                                                                                                                            cggcggTCATTTTTTTTTTTTCTGCCCTGAGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGGTTAAGTTTTCGCGTACCCGGCGGTTTGTTATTGGGGATGATGATATTTGAGCTCGGCTGATTGGt               >  2:513278/1‑140 (MQ=255)
                                                                                                                               cggTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCa            <  1:602364/140‑1 (MQ=255)
                                                                                                                                         ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:810620/140‑1 (MQ=255)
                                                                                                                                         ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:660705/140‑1 (MQ=255)
                                                                                                                                         ttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:497726/140‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604205‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑