Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,173,059	T→C	V177A (GTT→GCT)	mdaB →	NADPH:quinone oxidoreductase MdaB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,173,059	0	T	C	100.0%	182.6 / NA	47	V177A (GTT→GCT)	mdaB	NADPH:quinone oxidoreductase MdaB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/26);  total (21/26)

CACCGAAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGTTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCG  >  NC_000913/3172925‑3173191                                                                                                                                       |                                                                                                                                     cACCGAAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTccc                                                                                                                                  >  1:521415/1‑139 (MQ=255) cACCGAAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTccc                                                                                                                                  <  2:521415/139‑1 (MQ=255)  aCCGAAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCTGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCg                                                                                                                                <  1:414187/140‑1 (MQ=255)  aCCGAAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCg                                                                                                                                <  1:172003/140‑1 (MQ=255)  aCCGAAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCg                                                                                                                                <  1:683783/140‑1 (MQ=255)      aaaaaGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCtat                                                                                                                            >  2:803258/1‑140 (MQ=255)       aaaaGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTccc                                                                                                                                  >  2:940136/1‑133 (MQ=255)       aaaaGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTccc                                                                                                                                  <  1:940136/133‑1 (MQ=255)          aGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTg                                                                                                                        <  1:313558/140‑1 (MQ=255)                 ttcttcCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAata                                                                                                                 >  2:16392/1‑140 (MQ=255)                                        cGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAAttt                                                                                          >  2:921555/1‑140 (MQ=255)                                        cGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACAGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAAttt                                                                                          >  2:238132/1‑140 (MQ=255)                                            gtgtATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTgg                                                                                      <  1:803258/140‑1 (MQ=255)                                                     ccGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTaga                                                                             >  2:664295/1‑140 (MQ=255)                                                     ccGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTaga                                                                             >  2:915862/1‑140 (MQ=255)                                                     ccGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTaga                                                                             >  2:180817/1‑140 (MQ=255)                                                       gTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGc                                                                           >  2:496313/1‑140 (MQ=255)                                                        ttCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCt                                                                          <  1:184735/140‑1 (MQ=255)                                                         tCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTc                                                                         >  2:292759/1‑140 (MQ=255)                                                         tCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTc                                                                         <  1:915862/140‑1 (MQ=255)                                                             tAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGc                                                                     <  1:188189/140‑1 (MQ=255)                                                              aaaGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCt                                                                    <  1:295386/140‑1 (MQ=255)                                                                  cAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAg                                                                <  1:921555/140‑1 (MQ=255)                                                                   aaaCCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGa                                                               <  2:553110/140‑1 (MQ=255)                                                                     aCCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAg                                                             >  2:561893/1‑140 (MQ=255)                                                                       cAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGa                                                           <  1:201741/140‑1 (MQ=255)                                                                       cAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGa                                                           <  1:263460/140‑1 (MQ=255)                                                                         aTTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGt                                                         <  2:290617/140‑1 (MQ=255)                                                                           tCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTa                                                       <  1:650278/140‑1 (MQ=255)                                                                                       aaCCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATg                                                 <  1:746696/134‑1 (MQ=255)                                                                                       aaCCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATg                                                 >  2:746696/1‑134 (MQ=255)                                                                                            cTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAAt                                      >  2:339152/1‑140 (MQ=255)                                                                                            cTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAAt                                      >  2:549049/1‑140 (MQ=255)                                                                                                 gACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAg                                 <  1:16392/140‑1 (MQ=255)                                                                                                 gACATTTATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAg                                 >  2:792093/1‑140 (MQ=255)                                                                                                     tttATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAAt                             <  1:130171/140‑1 (MQ=255)                                                                                                      ttATCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATc                            <  1:561893/140‑1 (MQ=255)                                                                                                         tCGCTAATGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGt                         >  1:444720/1‑140 (MQ=255)                                                                                                             tAATGACGTGATAAAAATGCCTGATGCTCCCC‑CTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCg                    >  2:859436/1‑140 (MQ=255)                                                                                                              aaTGACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCg                    <  2:444720/140‑1 (MQ=255)                                                                                                                 gACGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTcc                 <  1:238132/140‑1 (MQ=255)                                                                                                                   cGTGATAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTg               <  1:180817/140‑1 (MQ=255)                                                                                                                    gTGATAAAAATGCCTGATGCTCCCC‑CTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGt             <  1:859436/140‑1 (MQ=255)                                                                                                                       aTAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCa           >  2:645127/1‑140 (MQ=255)                                                                                                                       aTAAAAATGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCa           <  1:645127/140‑1 (MQ=255)                                                                                                                              tGCCTGATGCTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCAc    >  2:869942/1‑140 (MQ=255)                                                                                                                                ccTGATGCTCACCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCg  >  1:651181/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                     CACCGAAAAAGATCAGTTCTTCCACGGCGTTGGCGTTGACGGTGTGTATCTGCCGTTCCATAAAGCAAACCAATTCCTCGGTATGGAACCGCTGCCGACATTTATCGCTAATGACGTGATAAAAATGCCTGATGTTCCCCGCTATACTGAAGAATATCGCAAGCATCTTGTGGAAATTTTTGGTTAACTAGAGCTCAGGCTTTAGAAGGAGTTAACCATGCTTACCGTAATCGCAGAAATCCGTACTCGTCCTGGTCAACATCACCG  >  NC_000913/3172925‑3173191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑