| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,213,599 | C→T | L185F (CTT→TTT) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,213,599 | 0 | C | T | 96.8% | 205.8 / NA | 63 | L185F (CTT→TTT) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (34/27); total (36/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGT > NC_000913/3213467‑3213735 | aTCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCa > 2:619203/1‑140 (MQ=255)aTCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCa > 1:172346/1‑140 (MQ=255) cACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCGCCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAgg < 1:696485/140‑1 (MQ=255) aTCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGAt < 2:662287/140‑1 (MQ=255) tgGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGTGCTTTCCCAAGAAGATCTGGAc > 2:1014781/1‑140 (MQ=255) tgGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAgg > 1:338590/1‑129 (MQ=255) tgGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAgg < 2:338590/129‑1 (MQ=255) tgGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGAc > 1:851068/1‑140 (MQ=255) ggAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACg < 2:287166/140‑1 (MQ=255) gTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACg < 2:699499/140‑1 (MQ=255) gTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACg < 1:455953/140‑1 (MQ=255) gTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCAGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACg > 2:232885/1‑140 (MQ=255) tACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGa > 2:247230/1‑140 (MQ=255) tACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGa > 1:844894/1‑140 (MQ=255) cGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAg > 2:1075474/1‑140 (MQ=255) tCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATg > 2:1114879/1‑140 (MQ=255) tgtTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAg > 2:530177/1‑140 (MQ=255) ttGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGAc > 1:821972/1‑140 (MQ=255) ttGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGAc > 1:588258/1‑140 (MQ=255) aaGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACgaa < 1:15194/140‑1 (MQ=255) aaGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACgaa < 1:619203/140‑1 (MQ=255) cgTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATga > 1:1011572/1‑140 (MQ=255) tCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATgacg > 1:204253/1‑140 (MQ=255) tCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATgacg > 1:850119/1‑140 (MQ=255) cTGTCCGATCTGATCACCGGCTTTGTTGCCCCGAACGCAGAAGAAGATCTGGCCCCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATgacga < 2:972357/140‑1 (MQ=255) cTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCGGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATgacga < 1:247230/140‑1 (MQ=255) tCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAg > 2:680419/1‑140 (MQ=255) aTCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCgatga > 1:556778/1‑140 (MQ=255) cGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGaca > 1:1163709/1‑140 (MQ=255) gCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGacaac > 1:915734/1‑140 (MQ=255) ttgttgACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGc > 1:556474/1‑140 (MQ=255) gttgACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCAt < 2:1011572/140‑1 (MQ=255) gttgACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGACGATGACAACAGCAt < 2:1078051/140‑1 (MQ=255) gttgACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTGTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCAt > 2:1153523/1‑140 (MQ=255) aacaaCCGGAAGGGAGAAGAACCTCAGGCACGTACCGCCACTCACGTCGGTTCTGAGTTTTACCAGGAAGATCTGGGCGATGACGAAGATGAAGACGAAGAAGATGGCCATTACGAGAGCGTAGAGGATGACAAcatcat < 2:917663/136‑1 (MQ=255) cccGAACGCAGAAGAAGATCTGGCCCCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGTAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGAcc < 2:770861/140‑1 (MQ=255) cccGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGAcc < 2:588258/140‑1 (MQ=255) aaCGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGa > 2:1140139/1‑140 (MQ=255) aaCGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGa > 2:347231/1‑140 (MQ=255) cGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAAc > 2:910517/1‑140 (MQ=255) aaaagaagaTTTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTgg < 1:232885/138‑1 (MQ=255) aagaagaTCTGGCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGatcctgtc > 1:917663/1‑133 (MQ=255) ggCACCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAAt > 2:316975/1‑140 (MQ=255) aCCTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTg > 2:786865/1‑140 (MQ=255) cTACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCg < 2:850119/140‑1 (MQ=255) tACCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCgg > 1:447360/1‑140 (MQ=255) aCCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGa > 1:1038342/1‑140 (MQ=255) aCCGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGa > 2:790863/1‑140 (MQ=255) ccGCCACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGaa > 2:60989/1‑140 (MQ=255) cACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTAc < 2:844894/140‑1 (MQ=255) cACTCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTAc < 2:915734/140‑1 (MQ=255) tCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTAcgcg < 1:420243/140‑1 (MQ=255) tCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTAcgcg > 1:1181810/1‑140 (MQ=255) tCACGTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTAcgcg < 1:677587/140‑1 (MQ=255) gTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCa > 1:31834/1‑140 (MQ=255) gTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCa < 2:468882/140‑1 (MQ=255) gTCGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCa < 1:448390/140‑1 (MQ=255) cGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGt < 2:556474/140‑1 (MQ=255) cGGTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGt < 2:31834/140‑1 (MQ=255) ggTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTa < 2:821972/140‑1 (MQ=255) ggTTCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTa < 2:1038342/140‑1 (MQ=255) ttCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACg > 1:503049/1‑140 (MQ=255) ttCTGAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATAACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAAATTGCGGAACTACGCGCTCAGTACg > 2:783604/1‑140 (MQ=255) gAGTTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCCCAGTACGTTGt < 2:447360/140‑1 (MQ=255) | ATCACCTATCTGCTGGAACAGTACGATCGTGTTGAAGCAGAAGAAGCGCGTCTGTCCGATCTGATCACCGGCTTTGTTGACCCGAACGCAGAAGAAGATCTGGCACCTACCGCCACTCACGTCGGTTCTGAGCTTTCCCAGGAAGATCTGGACGATGACGAAGATGAAGACGAAGAAGATGGCGATGACGACAGCGCCGATGATGACAACAGCATCGACCCGGAACTGGCTCGCGAAAAATTTGCGGAACTACGCGCTCAGTACGTTGT > NC_000913/3213467‑3213735 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |