Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,306,814	G→A	intergenic (‑60/+158)	mqo ← / ← yojI	malate:quinone oxidoreductase/ABC transporter family protein/microcin J25 efflux protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,306,814	0	G	A	100.0%	94.4 / NA	27	intergenic (‑60/+158)	mqo/yojI	malate:quinone oxidoreductase/ABC transporter family protein/microcin J25 efflux protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/17);  total (10/17)

TTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAG  >  NC_000913/2306681‑2306945                                                                                                                                      |                                                                                                                                    ttcgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAacac                                                                                                                               <  1:612889/140‑1 (MQ=255)  tcgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACt                                                                                                                              <  1:1079195/140‑1 (MQ=255)   cgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACtt                                                                                                                             >  2:482837/1‑140 (MQ=255)   cgctttTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACtt                                                                                                                             <  1:292869/140‑1 (MQ=255)          gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTaac                                                                                                                      >  2:87213/1‑140 (MQ=255)          gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTaac                                                                                                                      <  1:850725/140‑1 (MQ=255)          gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTaac                                                                                                                      <  1:414712/140‑1 (MQ=255)          gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTaac                                                                                                                      <  1:482837/140‑1 (MQ=255)          gccgccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTaac                                                                                                                      <  1:743127/140‑1 (MQ=255)              ccATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAacaaca                                                                                                                  <  1:87213/140‑1 (MQ=255)                aTCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTaac                                                                                                                      >  1:1017402/1‑134 (MQ=255)                aTCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTaac                                                                                                                      <  2:1017402/134‑1 (MQ=255)                      aCGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAaata                                                                                                          <  2:352613/140‑1 (MQ=255)                      aCGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAaata                                                                                                          <  1:2073/140‑1 (MQ=255)                      aCGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAaata                                                                                                          <  2:524636/140‑1 (MQ=255)                                                                     tttCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTg                                                           <  2:195364/140‑1 (MQ=255)                                                                       tCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGat                                                         <  2:629045/140‑1 (MQ=255)                                                                            ttGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGat                                                         >  1:54848/1‑135 (MQ=255)                                                                            ttGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGat                                                         <  2:54848/135‑1 (MQ=255)                                                                                  aTGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAggg                                              <  1:720303/140‑1 (MQ=255)                                                                                         aCTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAg                                       >  1:312052/1‑140 (MQ=255)                                                                                                gTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGaa                                >  2:390532/1‑140 (MQ=255)                                                                                                 tCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAg                               >  2:233459/1‑140 (MQ=255)                                                                                                         ttGCAGGTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAA‑TTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTa                      <  2:72789/140‑1 (MQ=255)                                                                                                               gTGAGATTTGCGCGGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCata                 >  2:84582/1‑140 (MQ=255)                                                                                                                         cgcgGCATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGAc       >  2:91915/1‑140 (MQ=255)                                                                                                                              cATCAACGATAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGgcag  >  1:124585/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                    TTCGCTTTTGCCGCCATCGAAACGGCATTAAGCCCCACGGCCATCGAGAAGAGCATGGCAGTCACTTTTTTCATCTTGTTAATGCCTTACTTTTAGTCGCTTTATTGCAGGTGAGATTTGCGCGGCATCAACGGTAACACTTAAGTAACAACATTTAAATAATGTTTATAAATTATATTCTAATTTTAGAAACATTATAATTTCGTTGATTAATTATAGGGTTATTAGAAATGAAGGGATTTTTAGCATATTGACCTGACGGCAG  >  NC_000913/2306681‑2306945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑