Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,088	(C)8→7	intergenic (‑85/+172)	iraM ← / ← ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,081	0	C	.	100.0%	163.3 / NA	38	intergenic (‑78/+179)	iraM/ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (26/12);  total (26/12)

TGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTT  >  NC_000913/1211947‑1212214                                                                                                                                       |                                                                                                                                      tGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAcccccc                                                                                                                                  >  1:625494/1‑140 (MQ=255) tGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAcccccc                                                                                                                                  >  1:620472/1‑140 (MQ=255)    aaaaGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccccctc                                                                                                                               >  1:436541/1‑138 (MQ=255)       aGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCaaa                                                                                                                           <  1:739928/140‑1 (MQ=255)          tataCCACATGTAGGTTGAATTACCGTGTCAA‑TACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCaaaaaaa                                                                                                                       >  2:723556/1‑140 (MQ=255)                           gAATTACCGTGTCAATTACTATCCAGTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                       <  1:658371/140‑1 (MQ=255)                           gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                       <  1:67570/140‑1 (MQ=255)                                  cGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCtt                                                                                                >  1:316166/1‑140 (MQ=255)                                      tCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTtatat                                                                                            >  1:706651/1‑140 (MQ=255)                                                     ttCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaaa                                                                             >  1:368860/1‑140 (MQ=255)                                                      tCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAg                                                                            <  2:436541/140‑1 (MQ=255)                                                       cATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGc                                                                           >  2:570183/1‑140 (MQ=255)                                                          ttGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAg                                                                        >  1:534838/1‑140 (MQ=255)                                                             ttATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGaa                                                                     <  1:570183/140‑1 (MQ=255)                                                               aTGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAtc                                                                   >  1:48172/1‑140 (MQ=255)                                                                            cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                              >  2:50240/1‑132 (MQ=255)                                                                            cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                              <  1:50240/132‑1 (MQ=255)                                                                            cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTa                                                      >  1:711600/1‑140 (MQ=255)                                                                                        tGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATgaga                                          <  2:711600/140‑1 (MQ=255)                                                                                         ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                         >  1:530455/1‑140 (MQ=255)                                                                                         ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                         >  1:54464/1‑140 (MQ=255)                                                                                           ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                       >  1:739445/1‑140 (MQ=255)                                                                                           ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                       >  2:126681/1‑140 (MQ=255)                                                                                           ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                       >  2:167094/1‑140 (MQ=255)                                                                                           ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                       >  2:180808/1‑140 (MQ=255)                                                                                             cATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTtg                                     >  2:2014/1‑140 (MQ=255)                                                                                              aTTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTtgt                                    >  1:326910/1‑140 (MQ=255)                                                                                                 aGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGaa                                 <  2:385290/140‑1 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGa                                  >  2:852155/1‑135 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGa                                  <  1:852155/135‑1 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                             >  2:37862/1‑140 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                             >  2:652754/1‑140 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                             >  2:68496/1‑140 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                             >  1:713759/1‑140 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                             >  2:849367/1‑140 (MQ=255)                                                                                                     tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                             >  1:54133/1‑140 (MQ=255)                                                                                                       tttATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAAt                           <  2:603302/140‑1 (MQ=255)                                                                                                                 gATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGa                 >  2:214594/1‑140 (MQ=255)                                                                                                                  aTTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGAc                <  2:379461/140‑1 (MQ=255)                                                                                                                              aGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGAt    <  2:620472/140‑1 (MQ=255)                                                                                                                                tataTTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGAttt  >  1:221459/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      TGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTT  >  NC_000913/1211947‑1212214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑