Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,904,926	Δ1 bp	coding (126/459 nt)	yobD →	conserved inner membrane protein YobD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,904,926	0	T	.	100.0%	193.3 / NA	45	coding (126/459 nt)	yobD	conserved inner membrane protein YobD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (27/18);  total (27/18)

TTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATA  >  NC_000913/1904798‑1905050                                                                                                                                 |                                                                                                                             ttAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGAt                                                                                                                  >  2:313001/1‑140 (MQ=255)          aTCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATc                                                                                                         >  2:458739/1‑140 (MQ=255)          aTCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATc                                                                                                         >  1:510655/1‑140 (MQ=255)           tCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCt                                                                                                        >  2:442555/1‑140 (MQ=255)                     ggTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGgatt                                                                                              >  2:185024/1‑140 (MQ=255)                           gATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGt                                                                                        >  1:61694/1‑140 (MQ=255)                                 tttCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCt                                                                                  >  1:348551/1‑140 (MQ=255)                                          cgcACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATaa                                                                         >  1:789572/1‑140 (MQ=255)                                                   ggCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCAc                                                                <  1:313001/140‑1 (MQ=255)                                                         cgcgATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCa                                                          >  1:552376/1‑140 (MQ=255)                                                         cgcgATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCa                                                          >  1:738250/1‑140 (MQ=255)                                                         cgcgATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCa                                                          >  2:243230/1‑140 (MQ=255)                                                         cgcgATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCa                                                          >  2:753522/1‑140 (MQ=255)                                                         cgcgATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCa                                                          >  1:262461/1‑140 (MQ=255)                                                             ttCTACGATCTTTTCATCATCCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGt                                                      <  2:789572/139‑1 (MQ=255)                                                                tACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCg                                                   <  2:738250/140‑1 (MQ=255)                                                                        gTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTTGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAAc                                           >  1:868395/1‑140 (MQ=255)                                                                        gTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAAc                                           >  1:712713/1‑140 (MQ=255)                                                                        gTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAAc                                           >  2:165447/1‑140 (MQ=255)                                                                          tcatcaTGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGa                                         >  2:23478/1‑140 (MQ=255)                                                                          tcatcaCGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGa                                         <  2:648078/140‑1 (MQ=255)                                                                            atcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                       <  2:61694/140‑1 (MQ=255)                                                                            atcatGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACa                                       <  1:185024/140‑1 (MQ=255)                                                                                 gCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGtt                                  >  2:543993/1‑140 (MQ=255)                                                                                 gCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGtt                                  >  1:864711/1‑140 (MQ=255)                                                                                 gCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGtt                                  <  1:753522/140‑1 (MQ=255)                                                                                 cccccGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGtt                                  <  2:712713/139‑1 (MQ=255)                                                                                   cccGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGttat                                >  2:165311/1‑140 (MQ=255)                                                                                   cccGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGttat                                >  2:783989/1‑140 (MQ=255)                                                                                   cccGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGttat                                >  1:305349/1‑140 (MQ=255)                                                                                    ccgccgTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGttatt                               <  2:262461/140‑1 (MQ=255)                                                                                    ccgccgTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGttatt                               <  2:348551/140‑1 (MQ=255)                                                                                       ccgTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAg                            <  1:442555/140‑1 (MQ=255)                                                                                           aaCGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCa                        <  1:165447/140‑1 (MQ=255)                                                                                           aaCGGCCCCACCCTGCTGGCAATCCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCa                        >  1:167015/1‑140 (MQ=255)                                                                                            aCGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAg                            >  2:698906/1‑135 (MQ=255)                                                                                            aCGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAg                            <  1:698906/135‑1 (MQ=255)                                                                                              ggCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTg                     <  1:243230/140‑1 (MQ=255)                                                                                                     cccTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGa              <  1:458739/140‑1 (MQ=255)                                                                                                      ccTGCTGGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGAt             <  2:305349/140‑1 (MQ=255)                                                                                                          ctgGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGt         >  1:556168/1‑140 (MQ=255)                                                                                                          ctgGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGt         >  2:771882/1‑140 (MQ=255)                                                                                                           tgGCAATTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGtt        <  1:350383/140‑1 (MQ=255)                                                                                                                aTTCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTtat   >  1:713555/1‑140 (MQ=255)                                                                                                                 ttCCTTTGCTCCGGCG‑GGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTtata  <  2:864711/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                             TTAATGACAATCACGGACCTGGTACTGATTCTTTTCATCGCCGCACTCCTGGCCTTCGCGATCTACGATCAGTTCATCATGCCCCGCCGTAACGGCCCCACCCTGCTGGCAATTCCTTTGCTCCGGCGTGGTCGCATCGATAGCGTTATCTTCGTCGGATTGATTGTCATTCTTATCTATAACAACGTCACGAATCATGGTGCGTTAATAACGACATGGTTATTAAGCGCACTGGCTCTGATGGGTTTTTATA  >  NC_000913/1904798‑1905050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑