Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,025,837	C→G	P284A (CCG→GCG)	ubiD →	3‑octaprenyl‑4‑hydroxybenzoate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,025,837	0	C	G	100.0%	234.3 / NA	60	P284A (CCG→GCG)	ubiD	3‑octaprenyl‑4‑hydroxybenzoate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (27/33);  total (27/33)

TTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGCCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCC  >  NC_000913/4025703‑4025964                                                                                                                                       |                                                                                                                                tttCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGaa                                                                                                                            <  1:884636/140‑1 (MQ=255)     agacTATGGGTTTGCCGGATTGCTACGTGGCACAAAGACAAAAGTGGTAAAGTGTTTCCCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTCTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGc                                                                                                                        <  2:578832/140‑1 (MQ=255)           tGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATg                                                                                                                  >  2:617369/1‑140 (MQ=255)                   ccGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACcac                                                                                                          >  1:234815/1‑140 (MQ=255)                   ccGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACcac                                                                                                          >  2:238715/1‑140 (MQ=255)                      gATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACAcc                                                                                                       <  1:841128/140‑1 (MQ=255)                        ttGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCgg                                                                                                     <  1:871625/140‑1 (MQ=255)                         tGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGt                                                                                                    <  1:850720/140‑1 (MQ=255)                         tGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGt                                                                                                    <  2:565638/140‑1 (MQ=255)                         tGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGt                                                                                                    <  2:234815/140‑1 (MQ=255)                         tGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGt                                                                                                    <  2:531214/140‑1 (MQ=255)                                tGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGAc                                                                                                             <  2:594284/124‑1 (MQ=255)                                tGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGAc                                                                                                             >  1:594284/1‑124 (MQ=255)                                tGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGt                                                                                                    <  2:497691/133‑1 (MQ=255)                                tGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGt                                                                                                    >  1:497691/1‑133 (MQ=255)                                   cACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATg                                                                                          >  2:531770/1‑140 (MQ=255)                                   cACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATg                                                                                          >  2:711146/1‑140 (MQ=255)                                        aGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTc                                                                                     >  2:492223/1‑140 (MQ=255)                                         gACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCg                                                                                    >  2:45561/1‑140 (MQ=255)                                         gACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCg                                                                                    >  1:113550/1‑140 (MQ=255)                                                 tggtgAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTc                                                                            <  2:113550/140‑1 (MQ=255)                                                  ggtgAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTcc                                                                           <  1:462676/140‑1 (MQ=255)                                                   gtgAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTccc                                                                          <  1:531770/140‑1 (MQ=255)                                                        gtgtATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTAt                                                                     >  1:745606/1‑140 (MQ=255)                                                           tATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTa                                                                  >  1:572175/1‑140 (MQ=255)                                                           tATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTa                                                                  <  2:44436/140‑1 (MQ=255)                                                           tATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTa                                                                  >  1:44436/1‑140 (MQ=255)                                                           tATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTa                                                                  >  2:883380/1‑140 (MQ=255)                                                             tctcCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTAcc                                                                >  1:171622/1‑140 (MQ=255)                                                             tctcCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTAcc                                                                >  2:280554/1‑140 (MQ=255)                                                             tctcCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTAcc                                                                >  1:56024/1‑140 (MQ=255)                                                              ctcCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCg                                                               >  1:587546/1‑140 (MQ=255)                                                                ccAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGtt                                                                              >  1:377051/1‑123 (MQ=255)                                                                ccAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGtt                                                                              <  2:377051/123‑1 (MQ=255)                                                                ccAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTg                                                             <  1:611555/140‑1 (MQ=255)                                                                  aaTGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGAc                                                           <  1:733955/140‑1 (MQ=255)                                                                  aaTGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGAc                                                           <  1:238715/140‑1 (MQ=255)                                                                  aaTGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGAc                                                           <  1:45561/140‑1 (MQ=255)                                                                  aaTGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGAc                                                           <  2:211846/140‑1 (MQ=255)                                                                       tCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCata                                                      <  2:82650/140‑1 (MQ=255)                                                                           gAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTg                                                             >  2:738218/1‑129 (MQ=255)                                                                           gAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTg                                                             <  1:738218/129‑1 (MQ=255)                                                                                 cccGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCg                                            >  2:890175/1‑140 (MQ=255)                                                                                 cccGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCg                                            >  1:183854/1‑140 (MQ=255)                                                                                 cccGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCg                                            >  1:199784/1‑140 (MQ=255)                                                                                   cGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGAc                                                           >  1:612587/1‑123 (MQ=255)                                                                                   cGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGAc                                                           <  2:612587/123‑1 (MQ=255)                                                                                         tGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATg                                    <  2:745606/140‑1 (MQ=255)                                                                                            ggAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGa                                 <  1:617369/140‑1 (MQ=255)                                                                                              agaTTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGAtt                               <  2:199784/140‑1 (MQ=255)                                                                                                    tgCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAt                         <  2:572175/140‑1 (MQ=255)                                                                                                    tgCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAt                         <  1:361737/140‑1 (MQ=255)                                                                                                    tgCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAt                         <  1:715534/140‑1 (MQ=255)                                                                                                    tgCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAt                         <  1:711146/140‑1 (MQ=255)                                                                                                    tgCTGGAAGGGTATATCGAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCAt                         <  2:329871/140‑1 (MQ=255)                                                                                                                     gAACAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCg        <  1:280554/140‑1 (MQ=255)                                                                                                                       aCAAGGCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTc      >  2:35944/1‑140 (MQ=255)                                                                                                                           ggCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTcctc                    <  2:622290/122‑3 (MQ=255)                                                                                                                           ggCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCAc                    >  1:622290/1‑122 (MQ=255)                                                                                                                           ggCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGcc  >  1:446550/1‑140 (MQ=255)                                                                                                                           ggCGAAACTGCGGCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGcc  >  1:60651/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                TTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGTGCTGGAAGGGTATATCGAACAAGGCGAAACTGCGCCGGAAGGGCCGTATGGCGACCACACCGGTTACTATAATGAAGTCGATAGTTTCCCGGTATTTACCGTGACGCATATTACCCAGCGTGAAGATGCGATTTACCATTCCACCTATACCGGGCGTCCGCC  >  NC_000913/4025703‑4025964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑