Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	745,356	A→C	E64D (GAA→GAC)	pxpA →	5‑oxoprolinase component A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	745,356	0	A	C	100.0%	150.5 / NA	39	E64D (GAA→GAC)	pxpA	5‑oxoprolinase component A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/17);  total (22/17)

TTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCAT  >  NC_000913/745222‑745487                                                                                                                                       |                                                                                                                                    ttAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAAttt                                                                                                                                >  2:610485/1‑140 (MQ=255) ttAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGT‑CGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAAtttt                                                                                                                               >  1:428782/1‑140 (MQ=255)      gCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTc                                                                                                                           >  2:718379/1‑140 (MQ=255)       cTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCg                                                                                                                          <  1:718379/140‑1 (MQ=255)        tGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGc                                                                                                                         >  2:856998/1‑140 (MQ=255)           tttCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGTGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAgc                                                                                                                      >  1:8081/1‑140 (MQ=255)           tttCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAgc                                                                                                                      >  2:679063/1‑140 (MQ=255)            ttCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGTGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAgcg                                                                                                                     <  2:8081/140‑1 (MQ=255)             tCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAgcgc                                                                                                                    >  1:595946/1‑140 (MQ=255)                tctGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCAt                                                                                                                 >  1:377212/1‑140 (MQ=255)                tctGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCAt                                                                                                                 >  2:406640/1‑140 (MQ=255)                tctGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCAt                                                                                                                 >  1:150733/1‑140 (MQ=255)                         atTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTgcc                                                                                                        >  1:206298/1‑140 (MQ=255)                                 tgGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGaaa                                                                                                <  2:595946/140‑1 (MQ=255)                                      ttCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGtt                                                                                           >  1:807349/1‑140 (MQ=255)                                             aGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGccc                                                                                    >  1:28872/1‑140 (MQ=255)                                                   tGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGc                                                                              <  2:150733/140‑1 (MQ=255)                                                         aaTCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATc                                                                        >  1:369426/1‑140 (MQ=255)                                                         aaTCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATc                                                                        >  1:264855/1‑140 (MQ=255)                                                         aaTCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCACGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATc                                                                        >  2:608360/1‑140 (MQ=255)                                                                      tgcgtgcgTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCt                                                           >  2:666124/1‑140 (MQ=255)                                                                          tgcgtgAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCa                                                       <  2:377212/140‑1 (MQ=255)                                                                               gAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGAt                                                  <  2:10777/140‑1 (MQ=255)                                                                                    aaTAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGccc                                             <  1:666124/140‑1 (MQ=255)                                                                                     aTAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCg                                            >  1:835046/1‑140 (MQ=255)                                                                                       aaaaaaTGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATACAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTg                                          <  1:406640/140‑1 (MQ=255)                                                                                           aaTGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCa                                      <  1:856998/140‑1 (MQ=255)                                                                                                  tCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAggcggc                               >  1:366954/1‑140 (MQ=255)                                                                                                          ggCGCTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCg                       <  2:807349/140‑1 (MQ=255)                                                                                                            cgcTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTc                     >  1:615508/1‑140 (MQ=255)                                                                                                            cgcTCACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTc                     >  2:752330/1‑140 (MQ=255)                                                                                                                cACCCGAGTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGt                 >  1:405923/1‑140 (MQ=255)                                                                                                                       gTTTTCCCGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCg          <  2:374981/140‑1 (MQ=255)                                                                                                                           tCCCGTCAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACg      <  2:206298/140‑1 (MQ=255)                                                                                                                             ccGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGc    <  1:752330/140‑1 (MQ=255)                                                                                                                              cGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCa   <  2:366954/140‑1 (MQ=255)                                                                                                                              cGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCa   <  1:336393/140‑1 (MQ=255)                                                                                                                              cGACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCa   <  1:31320/140‑1 (MQ=255)                                                                                                                               gACAGGGACAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCAt  <  1:444615/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                    TTAACGCTGGTTTCCTCTGCCAATATTGCCTGTGGATTTCATGCAGGCGATGCGCAAATCATGCAGGCTTGCGTGCGTGAAGCAATAAAAAATGGTGTCGCGATTGGCGCTCACCCGAGTTTTCCCGACAGGGAAAATTTTGGTCGCAGCGCCATGCAGCTGCCGCCAGAAACCGTTTACGCCCAGACGCTGTATCAAATTGGCGCGCTGGCAACGATTGCCCGTGCGCAAGGCGGCGTAATGCGTCATGTCAAACCGCACGGCAT  >  NC_000913/745222‑745487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑