| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,877,664 | 0 | A | G | 24.5% | 96.0 / 15.9 | 53 | intergenic (+46/+905) | iap/cas2 | alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2 |
| Reads supporting (aligned to +/- strand): ref base A (10/30); new base G (13/0); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCGGGGAACTCTCGGTTCAGG > NC_000913/2877527‑2877800 | aTAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAAc > 1:325836/1‑140 (MQ=255)aTAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAAc > 2:423772/1‑140 (MQ=255) aGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTcc < 1:423772/140‑1 (MQ=255) ggCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCa > 1:900393/1‑140 (MQ=255) ggCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCa > 2:1157079/1‑140 (MQ=255) gCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCaa < 1:278020/140‑1 (MQ=255) tctTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAAc < 1:146278/133‑1 (MQ=255) tctTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAAc > 2:146278/1‑133 (MQ=255) tctTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGt > 1:815796/1‑140 (MQ=255) ttCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGa > 2:835165/1‑140 (MQ=255) gaagaaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCca < 2:683522/140‑1 (MQ=255) gaagaaTAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCca < 2:288375/140‑1 (MQ=255) aaCGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑t < 2:203171/140‑1 (MQ=255) tCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAg < 1:1188614/140‑1 (MQ=255) tCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAg < 1:1238275/140‑1 (MQ=255) cacTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGt > 2:359847/3‑140 (MQ=255) cGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGt > 2:441997/1‑140 (MQ=255) aCGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGccccggt > 2:612336/1‑136 (MQ=255) cGGATAATGCTGCCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCg < 2:1140398/140‑1 (MQ=255) tAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGa > 1:226803/1‑140 (MQ=255) aaTGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGAt > 1:719800/1‑140 (MQ=255) aTGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATACATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATg < 1:1273877/140‑1 (MQ=255) gCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCg < 1:612336/140‑1 (MQ=255) tACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCggg < 2:325836/140‑1 (MQ=255) ccTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACCCCCAGGGGTATACATCATCCCACTCCA‑TGCGGCCGGTTTTTTCCCGCTGAGGCggggg > 2:1273877/1‑139 (MQ=255) ctGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCCAGTGATATCCATCATCCCA‑TCCCGTGCGCCCGGGTTATCCCCGCCGGTGCCGGGAaca > 2:25475/1‑140 (MQ=255) ctGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAaca > 1:676958/1‑140 (MQ=255) ctGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAaca > 2:163851/1‑140 (MQ=255) ctGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAaca < 2:900393/140‑1 (MQ=255) ctGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAaca < 2:1081299/140‑1 (MQ=255) ggTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCCGTTTATCCCCCCCGATGCGGGGGACAcc > 2:635308/1‑140 (MQ=255) ggTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACAcc > 1:866780/1‑140 (MQ=255) gTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAaca > 1:1042288/1‑137 (MQ=255) gTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAaca < 2:1042288/137‑1 (MQ=255) gTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCa > 1:521887/1‑140 (MQ=255) tGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCgggg > 2:721863/1‑123 (MQ=255) tGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGGGATATCCATCATCGCA‑TCCAGTGGGCCCGGTTTATCCCCGCTGATGCGGGGGACACCAGCGGCAGGCg > 2:88952/1‑140 (MQ=255) tGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCgggg < 1:721863/123‑1 (MQ=255) tGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCg > 1:850620/1‑140 (MQ=255) ggCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGGTTATCCCCGCTGATGCGGGGa > 2:828671/1‑123 (MQ=255) ggCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGa < 1:828671/123‑1 (MQ=255) aGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAAt > 2:9000/1‑140 (MQ=255) ggCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGGACTCCAAGGGATATCCATCATCGCA‑TCCAGTGCGCCCCGGTTATCCCCGCCGGTGGGGGGAAAACCAGCGTCACGCGGGAAAtt > 2:1116869/1‑139 (MQ=255) ggTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTg < 1:88952/140‑1 (MQ=255) ggTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTg < 1:359847/140‑1 (MQ=255) gTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGc < 1:835165/140‑1 (MQ=255) gTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGc < 2:676958/140‑1 (MQ=255) gAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGt < 2:521887/140‑1 (MQ=255) gAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGt < 1:1157079/140‑1 (MQ=255) gAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGgcg < 2:866780/140‑1 (MQ=255) aGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGgcgc < 1:174943/140‑1 (MQ=255) cTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCgggg < 2:719800/140‑1 (MQ=255) cTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCgggg < 1:25475/140‑1 (MQ=255) tACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCGGGGa < 2:815796/140‑1 (MQ=255) tACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCGGGGa < 1:1116869/140‑1 (MQ=255) cAGAGGCGGGGGGACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGGCAGGCGTGAAAACTCACCGTCGTTGCCGGGTTATCCCTGCTGGCGCGGGGGActcc > 2:822015/1‑139 (MQ=255) ggggAACTCCCAACGATATCCATCCTCCCC‑TCCACTGCGCCCCGGTTATCCCCCCCGTTGCGGGGAACACCCACGTCAGGCGTTTAATCTCCCCCTCGTTGCCGGTTTATCCCTTCTGGCGGGGGGGACTCTCGGtttcc > 2:334800/1‑137 (MQ=255) ggggAACTCCAAGTGATATCCATCATCGCA‑TCCCGTGCGCCCGGGTTTTCCCCCCCGATGCGGGGGACACCAGCGTCAGGCGTGAAAACTCCACGTCGTTGCCGGGTTATCCCTGCTGGCGCGGGGGAATCTCGGGTccg > 2:513708/1‑138 (MQ=255) gggAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCTGTTTATCCCTGCTGGCGCGGGGAACTCTCGGTTCAgg < 2:226803/140‑1 (MQ=255) | ATAAGGCTCTTCCTGGAAGAATAGAACGTCGCTGCCGTGACGTTATGCGGATAATGCTACCTCTGGTGAAGGAGTTGGCGAAGGCGTCTTGATGGGTTTGAAAATGGGAGCTGGGAGTTCTACCGCAGAGGCGGGGGAACTCCAAGTGATATCCATCATCGCA‑TCCAGTGCGCCCGGTTTATCCCCGCTGATGCGGGGAACACCAGCGTCAGGCGTGAAATCTCACCGTCGTTGCCGGTTTATCCCTGCTGGCGCGGGGAACTCTCGGTTCAGG > NC_000913/2877527‑2877800 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |