Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2050AC36.7% 36.6 / 10.1 30S215R (AGT→AGG) ‡rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base A (18/1);  new base C (0/11);  total (18/12)
Fisher's exact test for biased strand distribution p-value = 2.20e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556071‑3556343
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ccGGCCCTGACGCGCTTTTCACCGACGAAAAAAAAGCGTTCGGTAATTTTTTCCCTTCCGACTTAAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CTccc                                                                                                                                       <  2:1102559/139‑1 (MQ=255)
    cACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGcc                                                                                                                                   >  1:470421/1‑140 (MQ=255)
        gACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAgtg                                                                                                                               >  1:1211959/1‑140 (MQ=255)
                   ccccGACAAAAAAAAAGCGTTGGGTAATTTTTTCCCTTTCGACTTCAAGGGAGACGTTATCCCCCGCCCCCTGGCCGCGCGGCAGTTTATAAATTTTCTGTTCATGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCa                                                                                                                    <  2:1279919/138‑1 (MQ=255)
                   cACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCa                                                                                                                    >  1:173582/1‑140 (MQ=255)
                     ccGACGAAAAAAAGGCGTTCGGTGATTTTTTCCCTTTCGCCTTCAAGCGAGACGTTATTCCCCGGCCCCTGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACg                                                                                                                  <  2:444624/140‑1 (MQ=255)
                                    ggTTGGGTGTTTTTTTCCCTTTCGACTGAAGGAGACAAGGTATTCCCCGCCCCCTGGTCGCGGGGCATTTTATGAATTTTCTTTTGAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                   <  2:611528/139‑1 (MQ=255)
                                    cGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGgcg                                                                                                   >  1:449668/1‑140 (MQ=255)
                                             attttttCATTTTCGACTTCAGCAGGGGCGGTATCACCCGCCCCCTGGCCCCGTGCCAGGTTATGAATATTTTTTTTATG‑AGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACAcc                                                                                          <  2:1093586/137‑1 (MQ=255)
                                              ttttttCTTTTTCGACTACAAGCGAGGCGTTTTTCCCCGGCCCCTGGTCGCGGGGCGGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                         <  2:423128/138‑1 (MQ=255)
                                              ttttttCCCTTTCGACTTAAGGCGAGAGGGTTTTCCCCGCCCCCGGGTCGCGGGGCAGTTTATAAATTTTCTTTTCAGGCAGGAAAACC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCa                                                                                         <  2:672495/138‑1 (MQ=255)
                                                          cGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGAGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACt                                                                             >  1:151456/1‑140 (MQ=255)
                                                                cAAGCGAGACGGTATTCCCCGGCCCCGGGCCGCGAGGCGGGTTATAAATTTTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       <  2:151456/140‑1 (MQ=255)
                                                                aaaGCGAGACGGTATTCCCCGGCCCCTGGCCGCGCGGCAGTTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCa                                                                       <  2:201311/139‑1 (MQ=255)
                                                                     gagcCGGTATTCCCCGGCCCCGGGTCGCGCGGCAGTTTATAAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                  <  2:449668/140‑1 (MQ=255)
                                                                     gagaGGATATTCCCCGGCCCCTGGCCGCGCGGCAGGTTATGAATATTCTGTTCATGAAGGAAAAAC‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCAt                                                                  <  2:1211959/140‑1 (MQ=255)
                                                                                       cccGGGTGGCGGGGCAGGTTAAAAATATTCTGTTCTTGCAGGAAAAGC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcgcg                                                <  1:303198/140‑1 (MQ=255)
                                                                                          tGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                             >  1:1274473/1‑140 (MQ=255)
                                                                                           ggTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCg                                            >  1:164614/1‑140 (MQ=255)
                                                                                                cgcgGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                       >  2:721072/1‑140 (MQ=255)
                                                                                                     gagtttATAAATTTTCTGTTCAGGAAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:653837/136‑1 (MQ=255)
                                                                                                     gaGGTTATGAATTTTCTGTTCATGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:164614/139‑1 (MQ=255)
                                                                                                     cAGTTTATAAATTTTTTTTTCAGGCGGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  2:591545/140‑1 (MQ=255)
                                                                                                     aaGGTAATAAATTTTCTGTAAACGAAGGAAAAAC‑CACCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  1:913224/139‑1 (MQ=255)
                                                                                                            taattatTCTGTTCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGACGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGgtgat                           <  2:408121/136‑3 (MQ=255)
                                                                                                                 atTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                      >  2:443366/1‑140 (MQ=255)
                                                                                                                  ttttGTTCAGGCAGGAAAACC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     <  2:303392/137‑1 (MQ=255)
                                                                                                                  ttCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  1:71270/1‑140 (MQ=255)
                                                                                                                  ttCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                     >  1:654110/1‑140 (MQ=255)
                                                                                                                   tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  1:1150302/1‑140 (MQ=255)
                                                                                                                   tCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                    >  2:633160/1‑140 (MQ=255)
                                                                                                                     tGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc                  >  1:478424/1‑140 (MQ=255)
                                                                                                                       ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  >  2:323662/1‑122 (MQ=255)
                                                                                                                       ttCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCa                                  <  1:323662/122‑1 (MQ=255)
                                                                                                                       ttCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  2:25510/140‑1 (MQ=255)
                                                                                                                       ttCAGGCAGGAAAAAC‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                <  2:71270/140‑1 (MQ=255)
                                                                                                                          tgggaGGGAAAAACCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACg              <  2:641735/136‑1 (MQ=255)
                                                                                                                          aTGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg             >  1:25510/1‑140 (MQ=255)
                                                                                                                           tGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACggg            >  2:897023/1‑140 (MQ=255)
                                                                                                                            gCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGc           >  1:303392/1‑140 (MQ=255)
                                                                                                                              acgaaaaac‑CCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCga         <  2:1150302/136‑1 (MQ=255)
                                                                                                                                     aa‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTcc  <  2:654110/140‑1 (MQ=255)
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TCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAA‑CTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCC  >  NC_000913/3556071‑3556343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑