Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,939,813	C→A	R127R (CGG→CGT)	lpxM ←	myristoyl‑acyl carrier protein‑dependent acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,939,813	0	C	A	100.0%	178.0 / NA	53	R127R (CGG→CGT)	lpxM	myristoyl‑acyl carrier protein‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/23);  total (30/23)

AGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCA  >  NC_000913/1939677‑1939933                                                                                                                                         |                                                                                                                         aGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTAcgacgc                                                                                                                       <  2:127673/140‑1 (MQ=255)   aCATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCAt                                                                                                                     <  1:984803/140‑1 (MQ=255)       aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCAtct                                                                                                                   <  2:952802/138‑1 (MQ=255)       aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCAtct                                                                                                                   >  1:952802/1‑138 (MQ=255)       aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCAt                                                                                                                     <  2:1129725/136‑1 (MQ=255)       aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCAt                                                                                                                     >  1:1129725/1‑136 (MQ=255)       aaTCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATctct                                                                                                                 >  2:635335/1‑140 (MQ=255)        aTCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCtt                                                                                                                >  2:125316/1‑140 (MQ=255)        aTCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCtt                                                                                                                <  1:1295764/140‑1 (MQ=255)            aaaaCCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCgat                                                                                                            <  1:869943/140‑1 (MQ=255)             aaaCCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCgatg                                                                                                           <  2:29760/140‑1 (MQ=255)               aCCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCgatgat                                                                                                         >  2:1136817/1‑140 (MQ=255)                      tGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGc                                                                                                  <  2:805116/140‑1 (MQ=255)                                      cATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAAcgc                                                                                  <  1:926292/140‑1 (MQ=255)                                       aTCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAAcgcg                                                                                 >  1:1218806/1‑140 (MQ=255)                                       aTCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAAcgcg                                                                                 >  1:1130978/1‑140 (MQ=255)                                           cTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGc                                                                             >  1:1295390/1‑140 (MQ=255)                                           cTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGc                                                                             >  2:440324/1‑140 (MQ=255)                                              ccATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGa                                                                          <  1:440324/140‑1 (MQ=255)                                                      tGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTtctc                                                                  >  2:911558/1‑140 (MQ=255)                                                      tGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTtctc                                                                  <  2:1130978/140‑1 (MQ=255)                                                        cccTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCg                                                                >  2:802306/1‑140 (MQ=255)                                                           ttGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGcc                                                             <  1:1136817/140‑1 (MQ=255)                                                            tGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGccc                                                            >  2:763471/1‑140 (MQ=255)                                                              cGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCcgc                                                          <  1:1013331/140‑1 (MQ=255)                                                                      tCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGcc                                                  >  1:1225324/1‑140 (MQ=255)                                                                        aGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCaa                                                <  1:125316/140‑1 (MQ=255)                                                                            tGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCa                                            >  1:807888/1‑140 (MQ=255)                                                                             ggCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACt                                              <  1:1090759/137‑1 (MQ=255)                                                                             ggCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACt                                              >  2:1090759/1‑137 (MQ=255)                                                                             ggCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAg                                           >  1:839674/1‑140 (MQ=255)                                                                              gCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGc                                          <  1:485623/140‑1 (MQ=255)                                                                               cAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGcc                                         <  1:635335/140‑1 (MQ=255)                                                                                    atatCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCcatcat                                    >  2:818796/1‑140 (MQ=255)                                                                                    atatCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCcatcat                                    >  1:764398/1‑140 (MQ=255)                                                                                       tCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGc                                 >  1:191420/1‑140 (MQ=255)                                                                                        cGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGcc                                >  2:52751/1‑140 (MQ=255)                                                                                             gCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGc                           >  2:1269680/1‑140 (MQ=255)                                                                                                cAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTg                        >  1:962741/1‑140 (MQ=255)                                                                                                 aaCCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTgc                       >  2:355692/1‑140 (MQ=255)                                                                                                 aaCCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTgc                       >  2:551846/1‑140 (MQ=255)                                                                                                     gTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTgcggcg                   <  2:764398/140‑1 (MQ=255)                                                                                                     gTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTgcggcg                   <  2:1218806/140‑1 (MQ=255)                                                                                                       gCGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGcgcg                 <  2:1295390/140‑1 (MQ=255)                                                                                                        cGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCgg                >  2:927901/1‑140 (MQ=255)                                                                                                        cGGCACCAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCgg                >  1:1185567/1‑140 (MQ=255)                                                                                                             ccAGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCa           >  2:352110/1‑140 (MQ=255)                                                                                                               aGAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCaaa         <  1:818796/140‑1 (MQ=255)                                                                                                                gAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGcgcg                 >  1:690183/1‑131 (MQ=255)                                                                                                                gAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGcgcg                 <  2:690183/131‑1 (MQ=255)                                                                                                                gAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAAc        <  1:927901/140‑1 (MQ=255)                                                                                                                gAAAGATAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAAc        >  2:972201/1‑140 (MQ=255)                                                                                                                     aTAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACAtctc   >  1:301119/1‑140 (MQ=255)                                                                                                                      tAACTTTCTCGTTATTACGACGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCtca  <  2:1185567/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                         AGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTATTACGCCGCATCTCTTCGATGATCTCCAGCCCTTGCCAGTCAACGCGCGGCTGAATTTTCTCCGGCCCGCGTATTGCCAACTCAGCCATCATTGCCATCGCTTGCGGCGCGGTGGCAAACATCTCA  >  NC_000913/1939677‑1939933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑