Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,821	T→C	G305G (GGT→GGC)	sdhA →	succinate:quinone oxidoreductase, FAD binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,821	0	T	C	100.0%	152.6 / NA	41	G305G (GGT→GGC)	sdhA	succinate:quinone oxidoreductase, FAD binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/24);  total (17/24)

AAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGG  >  NC_000913/756685‑756951                                                                                                                                         |                                                                                                                                   aaGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCg                                                                                                                                 >  1:911140/1‑140 (MQ=255)   ggCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTg                                                                                                                               <  2:431509/140‑1 (MQ=255)          aTCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCca                                                                                                                        <  2:1191154/140‑1 (MQ=255)              gctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACAcg                                                                                                                    <  2:169352/140‑1 (MQ=255)              gctgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGGGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGGTGGCCCGTGGGGGCCacaca                                                                                                                    >  2:36653/1‑139 (MQ=255)                tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACAcgcg                                                                                                                  <  2:1045393/140‑1 (MQ=255)                tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACAcgcg                                                                                                                  <  1:622674/140‑1 (MQ=255)                tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACAcgcg                                                                                                                  <  2:1282101/140‑1 (MQ=255)                  aacaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCgaa                                                                                                                >  2:656856/1‑140 (MQ=255)                    caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGGCCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACAcgcg                                                                                                                  <  1:563941/136‑1 (MQ=255)                    caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACAcgcg                                                                                                                  >  2:563941/1‑136 (MQ=255)                        caTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCgaaactgaa                                                                                                          <  1:748274/140‑1 (MQ=255)                        caTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCgaaactgaa                                                                                                          <  1:498191/140‑1 (MQ=255)                         aTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCgaaactgaaa                                                                                                         >  2:394633/1‑140 (MQ=255)                               aaCGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGAt                                                                                                   <  2:839153/140‑1 (MQ=255)                                 cGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCa                                                                                                 >  1:318576/1‑140 (MQ=255)                                  gTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCAc                                                                                                <  2:510476/140‑1 (MQ=255)                                  gTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCAc                                                                                                <  1:1106303/140‑1 (MQ=255)                                  gTTTTATGGAGCGTTATCCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCAc                                                                                                <  2:887900/140‑1 (MQ=255)                                    tttATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCt                                                                                              <  1:394633/140‑1 (MQ=255)                                    tttATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCt                                                                                              <  1:31071/140‑1 (MQ=255)                                            gCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaag                                                                                      >  1:161241/1‑140 (MQ=255)                                             cGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAaaga                                                                                     <  2:318576/140‑1 (MQ=255)                                                       cGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGaa                                                                           >  2:441960/1‑140 (MQ=255)                                                        gAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAAt                                                                          <  1:36982/140‑1 (MQ=255)                                                         aaCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATc                                                                         >  2:48629/1‑140 (MQ=255)                                                         aaCGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACCCGATCACCTGGGCAAAGAAGTTCTCGAATc                                                                         >  2:160427/1‑140 (MQ=255)                                                                    ccTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCgg                                                              <  1:26789/140‑1 (MQ=255)                                                                      tGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGt                                                            >  1:192782/1‑140 (MQ=255)                                                                      tGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGt                                                            >  2:1266861/1‑140 (MQ=255)                                                                               gTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAg                                                   <  1:1032408/140‑1 (MQ=255)                                                                                           cgcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTAcc                                       <  1:656856/140‑1 (MQ=255)                                                                                            gcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCt                                      >  2:1206333/1‑140 (MQ=255)                                                                                                tCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGc                                  >  1:1280625/1‑140 (MQ=255)                                                                                                 ccATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCt                                 <  1:441960/140‑1 (MQ=255)                                                                                                          tCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt                        >  1:740955/1‑140 (MQ=255)                                                                                                          tCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGAt                        >  1:105218/1‑140 (MQ=255)                                                                                                          tCGAAATCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCACTCACGTCGAt                        >  2:511730/1‑140 (MQ=255)                                                                                                             aaaTCCGTGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                     <  1:1266861/140‑1 (MQ=255)                                                                                                                    tGAAGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAag              >  1:819194/1‑140 (MQ=255)                                                                                                                      aaGGTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCAGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAagag            <  2:1139536/140‑1 (MQ=255)                                                                                                                         gTCGCGGCTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCg         >  2:487562/1‑140 (MQ=255)                                                                                                                                cTGTGATGGCCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCgg  <  2:295821/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   AAGGCGGTTATCTGCTGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGG  >  NC_000913/756685‑756951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑