Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	996,766	C→T	W60* (TGG→TAG)	ssuA ←	aliphatic sulfonate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	996,766	0	C	T	100.0%	230.9 / NA	68	W60* (TGG→TAG)	ssuA	aliphatic sulfonate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (34/34);  total (34/34)

CGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGAC  >  NC_000913/996638‑996902                                                                                                                                 |                                                                                                                                         cGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGAttttt                                                                                                                               >  1:943226/1‑140 (MQ=255)  gCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTg                                                                                                                              >  1:12508/1‑140 (MQ=255)              cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGc                                                                                                                  >  1:751558/1‑140 (MQ=255)              cAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGc                                                                                                                  >  1:376586/1‑140 (MQ=255)                aaTCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGt                                                                                                                <  1:311539/140‑1 (MQ=255)                     gCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTc                                                                                                           <  2:943226/140‑1 (MQ=255)                      ccccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTcc                                                                                                          >  2:685649/1‑140 (MQ=255)                      ccccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTcc                                                                                                          >  2:1068353/1‑140 (MQ=255)                      ccccGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTcc                                                                                                          >  1:1236666/1‑140 (MQ=255)                          ggCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTa                                                                                                      >  1:739164/1‑140 (MQ=255)                          ggCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTa                                                                                                      >  1:509160/1‑140 (MQ=255)                          ggCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTa                                                                                                      <  1:225743/140‑1 (MQ=255)                          ggCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTa                                                                                                      >  2:504367/1‑140 (MQ=255)                                cTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACtggt                                                                                                <  2:1236666/140‑1 (MQ=255)                                cTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACtggt                                                                                                >  2:881153/1‑140 (MQ=255)                                     cAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCtt                                                                                           <  1:1068353/140‑1 (MQ=255)                                        aGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCttttt                                                                                        >  1:638488/1‑140 (MQ=255)                                        aGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGAAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCttttt                                                                                        >  2:1181058/1‑140 (MQ=255)                                          aTTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGc                                                                                      <  2:1200829/140‑1 (MQ=255)                                          aTTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGc                                                                                      <  1:630792/140‑1 (MQ=255)                                           ttGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGcc                                                                                     <  2:12508/140‑1 (MQ=255)                                           ttGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGcc                                                                                     <  1:921212/140‑1 (MQ=255)                                             ggCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAg                                                                                   <  2:1068169/140‑1 (MQ=255)                                             ggCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAg                                                                                   >  1:1068169/1‑140 (MQ=255)                                             ggCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAg                                                                                   <  1:1033871/140‑1 (MQ=255)                                              gCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGc                                                                                  <  2:739164/140‑1 (MQ=255)                                                ggAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCAc                                                                                <  2:509160/140‑1 (MQ=255)                                                ggAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCAc                                                                                <  1:1021418/140‑1 (MQ=255)                                                ggAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGAAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCAc                                                                                <  1:1181058/140‑1 (MQ=255)                                                    tatCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATa                                                                            >  2:430212/1‑140 (MQ=255)                                                    tatCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATa                                                                            <  2:243622/140‑1 (MQ=255)                                                     atCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGc                                                                                      <  1:1099556/129‑1 (MQ=255)                                                     atCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGc                                                                                      >  2:1099556/1‑129 (MQ=255)                                                        cccGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCaa                                                                        <  2:751558/140‑1 (MQ=255)                                                            gTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACt                                                                    <  1:685649/140‑1 (MQ=255)                                                             tACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTg                                                                   >  2:521332/1‑140 (MQ=255)                                                                tGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCt                                                                >  1:749433/1‑140 (MQ=255)                                                                tGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCt                                                                >  1:872981/1‑140 (MQ=255)                                                                  ccGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATAc                                                                           <  1:1019503/127‑1 (MQ=255)                                                                  ccGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATAc                                                                           >  2:1019503/1‑127 (MQ=255)                                                                     agaTCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTg                                                           <  2:749433/140‑1 (MQ=255)                                                                      gaTCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGa                                                          <  2:638488/140‑1 (MQ=255)                                                                      gaTCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGa                                                          <  1:113003/140‑1 (MQ=255)                                                                       aTCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGAt                                                         <  1:430212/140‑1 (MQ=255)                                                                       aTCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGAt                                                         <  2:143337/140‑1 (MQ=255)                                                                                 ccAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGTCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACg                                               >  1:1291361/1‑140 (MQ=255)                                                                                 ccAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACg                                               >  2:176532/1‑140 (MQ=255)                                                                                 ccAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACg                                               >  2:329891/1‑140 (MQ=255)                                                                                 ccAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACg                                               >  2:1143948/1‑140 (MQ=255)                                                                                 ccAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACg                                               >  2:1024244/1‑140 (MQ=255)                                                                                    aCGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTg                                                           >  2:998443/1‑125 (MQ=255)                                                                                    aCGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTg                                                           <  1:998443/125‑1 (MQ=255)                                                                                    aCGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTaa                                            <  1:939519/140‑1 (MQ=255)                                                                                     cGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAAc                                           >  2:1199311/1‑140 (MQ=255)                                                                                      gTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACg                                          >  2:853850/1‑140 (MQ=255)                                                                                       tttAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGc                                         >  1:833313/1‑140 (MQ=255)                                                                                         tAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCtt                                       <  1:1078688/140‑1 (MQ=255)                                                                                              cTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATCCTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGc                                  <  1:504367/140‑1 (MQ=255)                                                                                              cTTCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGc                                  <  1:881153/140‑1 (MQ=255)                                                                                                tCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGc                                  >  1:1128646/1‑138 (MQ=255)                                                                                                tCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGc                                  <  2:1128646/138‑1 (MQ=255)                                                                                                tCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGa                                >  1:169609/1‑140 (MQ=255)                                                                                                tCCAACATTTGCGGACCCGCGGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGa                                >  2:897329/1‑140 (MQ=255)                                                                                                                  gcgGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGTCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGc              <  2:1291361/140‑1 (MQ=255)                                                                                                                  gcgGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGc              >  1:1180375/1‑140 (MQ=255)                                                                                                                   cgGGGAACTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGCa             >  1:392186/1‑140 (MQ=255)                                                                                                                          cTCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTag      >  1:192123/1‑140 (MQ=255)                                                                                                                           tCCACCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTaga     <  1:1143948/140‑1 (MQ=255)                                                                                                                              aCCTAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGAc  <  1:897329/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         CGCCCACGTACACCAAATCAGCCCCGGCAGCCTGGGCAAAGATTGGCGGAATATCCCCGGTACTGCCGAGATCAATACTGCCAACGTTTAACGCTTCCAACATTTGCGGACCCGCGGGGAACTCCACCCAGGAGATTTTTGATTCCGGATAGCGTTTTTCCAGTAACTGGTGGCTTTTTGCCAGCACCATACCAATACTGCCTTTCTGATAGCCTATACGTAACGCTTCAGGCGAGGATTCTGCAGCAACCGCAAAAGTAGAGAC  >  NC_000913/996638‑996902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑