Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,605,955	Δ2 bp	coding (1145‑1146/1440 nt)	hyfD →	hydrogenase 4 component D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,605,954	0	G	.	100.0%	203.9 / NA	48	coding (1144/1440 nt)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (29/19);  total (29/19)
*	NC_000913	2,605,955	0	C	.	100.0%	203.9 / NA	48	coding (1145/1440 nt)	hyfD	hydrogenase 4 component D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (29/19);  total (29/19)

GCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCG  >  NC_000913/2605819‑2606083                                                                                                                                        ||                                                                                                                                 gcgTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTggatta                                                                                                                               >  1:637930/1‑136 (MQ=255)       gCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGATTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCg                                                                                                                       <  1:562806/140‑1 (MQ=255)            aaGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTgccg                                                                                                                  >  2:535972/1‑140 (MQ=255)                   tttttCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCa                                                                                                           >  1:577476/1‑140 (MQ=255)                             tAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTgccg                                                                                                                  >  1:854168/1‑123 (MQ=255)                             tAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTgccg                                                                                                                  <  2:854168/123‑1 (MQ=255)                                   gTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGGGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTaa                                                                                           >  1:325224/1‑140 (MQ=255)                                          gAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCg                                                                                    >  2:276011/1‑140 (MQ=255)                                                cAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGttt                                                                              >  1:653647/1‑140 (MQ=255)                                                       ggCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCg                                                                       >  2:65409/1‑140 (MQ=255)                                                       ggCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCg                                                                       >  1:734128/1‑140 (MQ=255)                                                             cgcTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTg                                                                 >  1:603560/1‑140 (MQ=255)                                                              gcTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGc                                                                <  1:221409/140‑1 (MQ=255)                                                                     gCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCa                                                         <  2:490167/140‑1 (MQ=255)                                                                      ccGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAg                                                        <  1:356127/140‑1 (MQ=255)                                                                      ccGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAg                                                        <  1:276011/140‑1 (MQ=255)                                                                        gcgTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTg                                                      <  1:622712/140‑1 (MQ=255)                                                                        gcgTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTg                                                      <  2:436211/140‑1 (MQ=255)                                                                        gcgTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTg                                                      <  1:535972/140‑1 (MQ=255)                                                                        gcgTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTg                                                      <  1:65409/140‑1 (MQ=255)                                                                         cgTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAg                                                        >  1:258763/1‑137 (MQ=255)                                                                         cgTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAg                                                        <  2:258763/137‑1 (MQ=255)                                                                          gTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGa                                                    >  2:170989/1‑140 (MQ=255)                                                                          gTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGa                                                    <  1:769411/140‑1 (MQ=255)                                                                             tGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCa                                                         >  1:735867/1‑132 (MQ=255)                                                                             tGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCa                                                         <  2:735867/132‑1 (MQ=255)                                                                             tGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTa                                                 >  1:634170/1‑140 (MQ=255)                                                                                    cGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATc                                          >  1:927570/1‑140 (MQ=255)                                                                                         tGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCctgct                                     >  2:185808/1‑140 (MQ=255)                                                                                          gCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAGCGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCctgctg                                    <  2:653647/140‑1 (MQ=255)                                                                                              acCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCg                                >  1:293730/1‑140 (MQ=255)                                                                                               cccTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGc                               <  2:328103/140‑1 (MQ=255)                                                                                                ccTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGcc                              >  2:259108/1‑140 (MQ=255)                                                                                                  tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCAt                            >  2:189661/1‑140 (MQ=255)                                                                                                  tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCAt                            >  2:881988/1‑140 (MQ=255)                                                                                                  tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCAt                            >  2:364967/1‑140 (MQ=255)                                                                                                  tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCAt                            >  2:385247/1‑140 (MQ=255)                                                                                                  tGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCAt                            >  2:939529/1‑140 (MQ=255)                                                                                                    ccGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCTCGCCATGa                          <  2:637930/140‑1 (MQ=255)                                                                                                    ccGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGa                          <  1:189661/140‑1 (MQ=255)                                                                                                      gTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGAtt                        <  2:603560/140‑1 (MQ=255)                                                                                                          ccAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGAttcttc                    >  2:464965/1‑140 (MQ=255)                                                                                                          ccAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGAttcttc                    >  1:778681/1‑140 (MQ=255)                                                                                                          ccAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCAGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGAttcttc                    >  2:802643/1‑140 (MQ=255)                                                                                                           cAGGCGTGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATtcttct                   <  1:464965/140‑1 (MQ=255)                                                                                                                 tGGGTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTgatgat             >  1:44220/1‑140 (MQ=255)                                                                                                                   ggTTTCTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTg           >  1:124952/1‑140 (MQ=255)                                                                                                                        cTGCGTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCg      >  1:602577/1‑140 (MQ=255)                                                                                                                            gTGGCAGCGCTG‑‑GATTCCCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATTATTCTTCTGATGATTGAATCGGTCg  <  2:227007/140‑1 (MQ=255)                                                                                                                            gTGGCAGCGCTG‑‑GATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCg  >  1:904003/1‑140 (MQ=255)                                                                                                                                        ||                                                                                                                                 GCGTTCGCTAAAAGCCTGTTTTTCCTTGTAGCAGGTGCGCTGAGTTACAGCTGCGGCACGCGCTTGTTGCCGCGTCTGCGTGGCGTATTGCACACCCTGCCGTTGCCAGGCGTGGGTTTCTGCGTGGCAGCGCTGGCGATTACCGGCGTGCCGCCGTTCAACGGCTTCTTCAGTAAATTCCCGCTGTTTGCTGCCGGTTTTGCGTTGTCAGTGGAGTACTGGATCCTGCTGCCCGCCATGATTCTTCTGATGATTGAATCGGTCG  >  NC_000913/2605819‑2606083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑