| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 650,809 | 0 | T | G | 43.2% | 29.6 / 18.6 | 37 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
| Reads supporting (aligned to +/- strand): ref base T (9/12); new base G (16/0); total (25/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.09e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATAT > NC_000913/650672‑650947 | cAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCt < 2:917309/140‑1 (MQ=255) ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCcgc > 2:78758/1‑140 (MQ=255) ttGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTTTTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCcgc > 1:137126/1‑140 (MQ=255) gCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGt > 2:857422/1‑140 (MQ=255) gTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTTTTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTACGAGCTGCCGCGTCCTGGCGGGAGGGGTCa > 1:733917/1‑140 (MQ=255) gTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGt < 1:78758/140‑1 (MQ=255) gggCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCt < 1:887158/140‑1 (MQ=255) cGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGGCCCTGCGGGAGGGGTCAAGCA‑GGGTTTGCa > 2:887158/1‑140 (MQ=255) tATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATg < 1:857422/140‑1 (MQ=255) atcatcACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGGTCTGCAAATAAAGc > 2:685698/1‑140 (MQ=255) cacaTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGt < 1:418271/140‑1 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGa < 1:52779/140‑1 (MQ=255) caTCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTAAAACA‑GGGTTTGCAAATAATGCAGCGTgg > 2:99019/1‑139 (MQ=255) tCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTTACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCAGGGTTTTGGAAA‑AATGCAGCggggct > 2:22030/1‑135 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCAAACA‑GGGTTTGCAAATAAAGCAGCGGGACTg > 2:52779/1‑140 (MQ=255) ccccAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGTTTTGCAAATAAAGCAGCGGGACTg > 2:418271/1‑140 (MQ=255) gACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGTGCTCCAACA‑GGGTCTTTAAAAAAAGCAGCGGGGCTGCAggg > 2:886644/1‑140 (MQ=255) tCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCA‑GGGTCTTCCAAAAATGCAGCGTGACTGCAGGGGcc > 2:579477/1‑140 (MQ=255) aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTTTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGTTTTGCAAAAAAAGCAGCGGGACTGCACGGGcccg > 2:546046/1‑138 (MQ=255) aaGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCA‑GGGTCTGCAAATAATGCAGCGTGACTGCAGGGACCAg > 1:734702/1‑140 (MQ=255) aGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAACA‑GGGTTTGCAAAAAATGCAGCGTGGCTGCAGGGGCCAgc > 2:295510/1‑140 (MQ=255) gggTGCCTGCAACGGCGGGCTGGGTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAACA‑GGGTCTTCAAATAAAGCAGCGTGGCTGCAGGGGCCAgcc > 2:233083/1‑139 (MQ=255) cTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTGACGAGCTGCCGCGTCCTGGCGGGGGGGCTCAAGCA‑GGGTCTGCAAATAATGCAGCGGGGCTGCAGGGGCCAGCGGcgcgg > 2:363505/1‑139 (MQ=255) tGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGAt < 1:169424/140‑1 (MQ=255) aaCGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGGCTTTTTTCGAGCTGCCGCGTCCTGGCGGGAGGGGTCCAGCA‑GGGTCTGGAAATAAAGTAGGGGGATTGCAGGGGCCAGGGGCGCGAttac > 2:169424/1‑137 (MQ=255) aTTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCTGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTc < 1:233083/140‑1 (MQ=255) ttCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGcc < 1:518203/140‑1 (MQ=255) aTTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCcagc > 1:672016/1‑140 (MQ=255) tCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGCGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGGTTTTCGCCAGCAGTTg > 2:693960/1‑140 (MQ=255) tttCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTAcc < 1:455898/140‑1 (MQ=255) tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg > 2:424405/1‑140 (MQ=255) tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg < 1:622584/140‑1 (MQ=255) tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg > 2:810165/1‑140 (MQ=255) tctcATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGGGCTCAAGCA‑GGGTCTGCAAATAATGCAGCGTGACTGCCGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCg > 2:432253/1‑140 (MQ=255) gggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGat > 2:353285/1‑140 (MQ=255) ggTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGata > 2:376659/1‑140 (MQ=255) gTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGatat < 1:376659/140‑1 (MQ=255) | CAACGCTGCTATTGATTTGCAGGTCGATATCCTGCGTATCGAGTGGGGCGATGCGTATCATCACATCCCCAGACTCAAGGGTGCCTGCAACGGCGGGCTGGTTTATTTTCATTTTTCACCTGTTTCTCATGCGGGGGTCTTTTGACGAGCTGCCGCGTCCTGGCGGGAGTGCTCAAGCA‑GGTTCTGCAAATAATGCAGCGTGACTGCAGGGACCAGCGGCGCGATAGCCGTGAGATCGTTTTTCGCCAGCAGTTGACGTACCCGGGAAGCGGATAT > NC_000913/650672‑650947 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |