| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,568,504 | 0 | T | G | 33.9% | 88.5 / 11.4 | 57 | T6P (ACC→CCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
| Reads supporting (aligned to +/- strand): ref base T (10/27); new base G (19/0); total (30/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAT > NC_000913/4568374‑4568639 | aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGTTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg < 1:927310/140‑1 (MQ=255)aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg > 1:751452/1‑140 (MQ=255)aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACg < 2:102238/140‑1 (MQ=255) gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaacaaa > 1:48356/1‑140 (MQ=255) gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGCATaaaaaa > 2:941975/1‑140 (MQ=255) cATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGCATaaaaaaa > 2:56494/1‑139 (MQ=255) aTTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAATGGCATaaaaaaaa > 2:328076/1‑138 (MQ=255) aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑cc < 2:300307/140‑1 (MQ=255) aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑cc < 2:12677/140‑1 (MQ=255) aCGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGt < 1:550811/140‑1 (MQ=255) aCGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACATGGCGTAAAAAAAAACT‑CCTTGt > 2:300690/1‑140 (MQ=255) cacaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGgag < 2:343116/140‑1 (MQ=255) caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAAAAACT‑CCTTGTCCGGGGaa > 2:174580/1‑140 (MQ=255) aTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAAAAACT‑CCCTTTCCGGAGaaa > 1:501637/1‑139 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAAAAAACAACT‑CCCTGTCCGGGGAACCCCAACg > 1:409719/1‑140 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGTGGGATAAAAAACAACT‑CCTTGTCCGGAGAAACCCAACg > 1:218544/1‑140 (MQ=255) aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAGGGGATAAAAAAAAACT‑CCTTGTTCGGAGAACCCCCACg > 2:22638/1‑140 (MQ=255) cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGCGGCATAAAAAACAACA‑CCTTCTCCGGAGGACCCCCACggg > 2:220168/1‑140 (MQ=255) cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAAAGCGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACggg > 1:122584/1‑140 (MQ=255) cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGCAAAAAAACATGGCATAACAAACAACT‑CCTTTTTCGGAGAAGCCCCACggg > 1:854763/1‑140 (MQ=255) ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTCAAAAAAAAGGGAATAAAAAAACACC‑CCTTGTTCATGGAAACCCCACgggg > 2:445311/1‑140 (MQ=255) ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACAAGGCCTAAAAAAAAAATCCCTTTGCGGGGGAACCCCAAgggg > 2:943570/1‑140 (MQ=255) ggCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCAGAAAAAAAAAAT‑CCTTTTCCCGAGAAACCCCACGGGGgaa > 2:889383/1‑140 (MQ=255) tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACg < 1:22638/140‑1 (MQ=255) tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACg < 1:445311/140‑1 (MQ=255) tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACg < 2:48356/140‑1 (MQ=255) gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTATCCGGAGAACCCCAACGGGGAAAAAGaa > 1:649815/1‑140 (MQ=255) aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGTGCATAAAAAACAACT‑CCTTGTCCGGAGAAACCCCACGGGGGAAAAGaaa > 2:27017/1‑140 (MQ=255) aGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGCATAAAAAAAAAAT‑CCCTGTCCGGAGAAACCCAACGGGGGAAACGAAAATc > 2:48206/1‑140 (MQ=255) gATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCa < 1:416491/140‑1 (MQ=255) gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATAcc < 1:114764/140‑1 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAACT‑CCTTTTCCGGGGAACCCCCACGGGGGAAAAGAAAATCaaaaacc > 2:770129/1‑135 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAACAAAAAACT‑CCTTCTCCGGAGAAACCCAACGGGGGAAACAAAAATCAATAccc > 2:114764/1‑139 (MQ=255) tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAGGGCATAACAAAAAACT‑CCTTGTCCGGAGAACCCCAACGGGGGAAAAGAAATTCAATAccc > 2:731236/1‑139 (MQ=255) cagcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGGATAAAAAAAAAAT‑CCCTTTTCGGGGAAACCCCACAGGGGAAAAGAAAATCAAAACCCTCGGGAAAGACg > 2:799379/1‑140 (MQ=255) caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAAATCAATACCGTCGGGAATGAAGaaa > 1:90459/1‑140 (MQ=255) aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATAAAAAAAAACA‑CCCTTTCCGGAGAACCCCCACGGGGGAAAAGAAAATCAATACCGTCGGGAATGACGaaaaa > 2:196894/1‑138 (MQ=255) aGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGc < 1:300690/140‑1 (MQ=255) aTACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGt < 1:32508/140‑1 (MQ=255) aTACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGt < 2:751452/140‑1 (MQ=255) tACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCAGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTg > 2:955401/1‑140 (MQ=255) cAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgag < 1:220168/140‑1 (MQ=255) aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga < 2:804270/140‑1 (MQ=255) aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga < 2:187194/140‑1 (MQ=255) aaaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgaga < 1:196894/140‑1 (MQ=255) tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAggg > 1:51577/1‑140 (MQ=255) tCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAACAAACAACT‑CCTTGTCCGGGGAACCCCAAAGGGGGAAAAGAAAATCAATAACGTCGGGAAAGACGAAAAGCAGGGGAGAAggg > 2:32508/1‑140 (MQ=255) gagacagcGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCaa < 1:65916/133‑1 (MQ=255) cGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAAc < 1:889383/140‑1 (MQ=255) ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTTGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga < 1:27017/140‑1 (MQ=255) ccATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACga < 1:943570/140‑1 (MQ=255) cATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACgag < 1:792133/140‑1 (MQ=255) cGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGGGGGATAAAAAAAAAAC‑CCTTGTTCCGGGAACCCCCACGGGGGAAAAGAAAATCAATAACCTCCGGAATGACGAAAAGCAGGGGGGAAGGGCAACGAGAcc > 2:597380/1‑139 (MQ=255) aaaCAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAAt < 2:187067/140‑1 (MQ=255) gCGTGGCGGCATGGCGGGGAAAAAAAAGCGGCCTAAAAAAAAAAT‑CCCTCTCCGGAGAAACCCAACCGGGGAAAAGAAAATCAATACCCTCGGGAAAGAAGAAAAGGAGGTGAGAAAGGCAACGAGAAAGCAACTGtctc > 2:65916/1‑137 (MQ=255) cGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAACAAAAAACT‑CCTTGTCCGGGGAACCCCCACGGGGAAAAAGAAAATCAAAAACGTCGGGAATGACGAAAAGCAGGGGAGAAGGGCAACGAGACTGCAAATGGACTg > 2:31335/1‑140 (MQ=255) ggcggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAAt < 2:501637/140‑1 (MQ=255) ggCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa < 2:879087/140‑1 (MQ=255) cGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAt < 1:31335/140‑1 (MQ=255) | AGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACT‑CCTTGTCCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAT > NC_000913/4568374‑4568639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |