Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	103,883	C→T	Y243Y (TAC→TAT)	ftsQ →	cell division protein FtsQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	103,883	0	C	T	100.0%	134.5 / NA	39	Y243Y (TAC→TAT)	ftsQ	cell division protein FtsQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/21);  total (18/21)

TTGACGCTGAATAACGATATTAAGCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTACGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTA  >  NC_000913/103755‑104014                                                                                                                                 |                                                                                                                                    ttGACGCTGAATAACGATATTAAGCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCg                                                                                                                          >  2:920774/1‑140 (MQ=255)  tGACGCTGAATAACGATATTAAGCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGt                                                                                                                         <  1:380840/140‑1 (MQ=255)             aaCGATATTAAGCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTgg                                                                                                              <  2:681876/140‑1 (MQ=255)               cGATATTAAGCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAg                                                                                                            <  1:102504/140‑1 (MQ=255)                        gCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAg                                                                                                   <  1:418998/140‑1 (MQ=255)                         cTCAATCATGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAgg                                                                                                  >  1:565141/1‑140 (MQ=255)                                        ggCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACTGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTgcc                                                                                   >  2:343107/1‑140 (MQ=255)                                        ggCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTgcc                                                                                   >  1:87697/1‑140 (MQ=255)                                        ggCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTgcc                                                                                   >  1:679959/1‑140 (MQ=255)                                        ggCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGAAGGCTGGGCGCCCTTgcc                                                                                   >  2:566752/1‑140 (MQ=255)                                            aTACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCa                                                                               >  1:603427/1‑140 (MQ=255)                                             tACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCag                                                                              >  2:244614/1‑140 (MQ=255)                                             tACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCag                                                                              >  2:530253/1‑140 (MQ=255)                                              aCGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACTGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCagt                                                                             <  1:343107/140‑2 (MQ=255)                                              aCGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCaga                                                                             <  2:87697/140‑1 (MQ=255)                                               cGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCagag                                                                            <  2:565141/140‑1 (MQ=255)                                                   gAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAAt                                                                        >  2:257385/1‑140 (MQ=255)                                                    aaaCGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATc                                                                       <  1:530253/140‑1 (MQ=255)                                                     aaCGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCt                                                                      >  2:415979/1‑140 (MQ=255)                                                      aCGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTa                                                                     <  2:707493/140‑1 (MQ=255)                                                        gTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACt                                                                   <  2:917941/140‑1 (MQ=255)                                                           tGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAg                                                                >  2:83518/1‑140 (MQ=255)                                                            ggCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGc                                                               >  2:500659/1‑140 (MQ=255)                                                            ggCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGc                                                               >  1:677024/1‑140 (MQ=255)                                                               tCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGcaac                                                            <  2:603427/140‑1 (MQ=255)                                                                         gAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATcaggc                                                  <  1:257385/140‑1 (MQ=255)                                                                            cTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATcaggcaca                                               <  2:679959/140‑1 (MQ=255)                                                                              ttATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATcaggcacagg                                             <  1:637834/140‑1 (MQ=255)                                                                              ttATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATcaggcacagg                                             <  2:677024/140‑1 (MQ=255)                                                                               tATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAAt                                                       <  1:726615/129‑1 (MQ=255)                                                                               tATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAAt                                                       >  2:726615/1‑129 (MQ=255)                                                                                   cGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGaa                                        <  1:500659/140‑1 (MQ=255)                                                                                          aCAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAAt                                 <  1:93027/140‑1 (MQ=255)                                                                                            agcagcagGCGCAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGa                               <  2:796257/140‑1 (MQ=255)                                                                                                     cgcAAACCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGa                      <  1:920774/140‑1 (MQ=255)                                                                                                          aCCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGAc                 >  1:239997/1‑140 (MQ=255)                                                                                                          aCCGATGGCAAACGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGAc                 >  1:688635/1‑140 (MQ=255)                                                                                                                     aCGGATTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACTGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGgt      >  1:441431/1‑140 (MQ=255)                                                                                                                         aTTAGCTATGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGgtagta  <  2:688635/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                    TTGACGCTGAATAACGATATTAAGCTCAATCTTGGCCGGGGCGATACGATGAAACGTTTGGCTCGCTTTGTAGAACTTTATCCGGTTTTACAGCAGCAGGCGCAAACCGATGGCAAACGGATTAGCTACGTTGATTTGCGTTATGACTCTGGAGCGGCAGTAGGCTGGGCGCCCTTGCCGCCAGAGGAATCTACTCAGCAACAAAATCAGGCACAGGCAGAACAACAATGATCAAGGCGACGGACAGAAAACTGGTAGTA  >  NC_000913/103755‑104014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑