Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,016,861	A→G	I268V (ATC→GTC)	ygfK →	putative oxidoreductase, Fe‑S subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,016,861	0	A	G	100.0%	214.2 / NA	55	I268V (ATC→GTC)	ygfK	putative oxidoreductase, Fe‑S subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (30/25);  total (30/25)

CCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTT  >  NC_000913/3016730‑3016992                                                                                                                                    |                                                                                                                                    cccTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGAt                                                                                                                             >  1:923229/1‑140 (MQ=255)           caTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGtt                                                                                                                   <  2:223355/140‑1 (MQ=255)           caTGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGtt                                                                                                                   >  1:617957/1‑140 (MQ=255)            aTGCACGGCTGTCCTCCGCATGAAATCGAAGACATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGttt                                                                                                                  <  1:705618/140‑1 (MQ=255)               cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCt                                                                                                                         <  2:622082/130‑1 (MQ=255)               cACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCt                                                                                                                         >  1:622082/1‑130 (MQ=255)                aCGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGt                                                                                                              >  2:577738/1‑140 (MQ=255)                    cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACATCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGt                                                                                                                    >  2:575395/1‑130 (MQ=255)                    cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACATCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGt                                                                                                                    <  1:575395/130‑1 (MQ=255)                    cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCg                                                                                                                >  2:648580/1‑134 (MQ=255)                    cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCg                                                                                                                <  1:648580/134‑1 (MQ=255)                    cTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACa                                                                                                          >  2:306574/1‑140 (MQ=255)                     tGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACAt                                                                                                         <  1:172559/140‑1 (MQ=255)                     tGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACAt                                                                                                         <  1:768233/140‑1 (MQ=255)                      gTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATa                                                                                                        <  2:923229/140‑1 (MQ=255)                       tcctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAg                                                                                                       >  2:431428/1‑140 (MQ=255)                         ctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCg                                                                                                                <  1:283573/129‑1 (MQ=255)                         ctccGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCg                                                                                                                >  2:283573/1‑129 (MQ=255)                            cGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCgg                                                                                                               >  2:807612/1‑127 (MQ=255)                            cGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCgg                                                                                                               <  1:807612/127‑1 (MQ=255)                            cGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCg                                                                                                                <  1:44295/126‑1 (MQ=255)                            cGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCg                                                                                                                >  2:44295/1‑126 (MQ=255)                            cGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTa                                                                                                  >  2:830447/1‑140 (MQ=255)                               aTGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTaaaa                                                                                               <  1:481000/140‑1 (MQ=255)                                tGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaag                                                                                              <  2:791502/140‑1 (MQ=255)                                  aaaTCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaagaa                                                                                            <  1:431428/140‑1 (MQ=255)                                   aaTCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAaagaag                                                                                           >  2:847069/1‑140 (MQ=255)                                      cGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTGGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGt                                                                                        <  2:562344/140‑1 (MQ=255)                                         aGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCAt                                                                                     >  2:278508/1‑140 (MQ=255)                                                tGCCGCTACATGCTGGAAGAAAAAGGGTTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCa                                                                              <  2:1834/140‑1 (MQ=255)                                                           gCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGc                                                                   >  2:32382/1‑140 (MQ=255)                                                                 agaaAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGc                                                             <  2:261335/140‑1 (MQ=255)                                                                         ggCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTg                                                     >  2:687450/1‑140 (MQ=255)                                                                          gCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTAAGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTgg                                                    >  1:397757/1‑140 (MQ=255)                                                                                    tttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGa                                          <  1:830447/140‑1 (MQ=255)                                                                                     ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGaa                                         <  1:278508/140‑1 (MQ=255)                                                                                     ttGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGaa                                         <  2:889515/140‑1 (MQ=255)                                                                                      tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc                                                         <  1:582635/123‑1 (MQ=255)                                                                                      tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGc                                                         >  2:582635/1‑123 (MQ=255)                                                                                      tgtgAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAAc                                        >  2:484575/1‑140 (MQ=255)                                                                                          aaaCTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCt                                    >  2:470843/1‑140 (MQ=255)                                                                                             cTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGAt                                 >  1:312634/1‑140 (MQ=255)                                                                                             cTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGAt                                 >  1:163057/1‑140 (MQ=255)                                                                                                aaCCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGc                              <  2:257734/140‑1 (MQ=255)                                                                                                 aCCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCa                             <  1:306574/140‑1 (MQ=255)                                                                                                        cTTACTGGGGTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCaa                      <  1:687450/140‑1 (MQ=255)                                                                                                                 gTACGCGCGTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAAt             >  2:178833/1‑140 (MQ=255)                                                                                                                    cgcgcgTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCAc          >  2:454267/1‑140 (MQ=255)                                                                                                                      cgcgTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTc        >  2:606641/1‑140 (MQ=255)                                                                                                                       gcgTGTTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCg       >  1:105929/1‑140 (MQ=255)                                                                                                                         gtgtTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGc     >  1:688652/1‑140 (MQ=255)                                                                                                                          tgtTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCt    >  2:598980/1‑140 (MQ=255)                                                                                                                          tgtTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCt    >  1:361530/1‑140 (MQ=255)                                                                                                                           gtTCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCtt   <  2:312634/140‑1 (MQ=255)                                                                                                                            ttCGTGAGGTCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCttt  >  1:807807/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                    CCCTCTCCACCATGCACGGCTGTCCTCCGCATGAAATCGAAGCCATTTGCCGCTACATGCTGGAAGAAAAAGGGCTCAACACCTTTGTGAAACTTAACCCGACCTTACTGGGGTACGCGCGTGTTCGTGAGATCCTCGATGTCTGCGGTTTCGGTTACATAGGCTTAAAAGAAGAGTCATTTGATCACGACCTCAAGCTGACGCAAGCACTGGAAATGCTGGAACGCCTGATGGCACTGGCAAAAGAAAAATCACTCGGCTTT  >  NC_000913/3016730‑3016992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑