Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,193,836	A→C	G151G (GGT→GGG)	nudJ ←	phosphatase NudJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,193,836	0	A	C	100.0%	94.7 / NA	25	G151G (GGT→GGG)	nudJ	phosphatase NudJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/11);  total (14/11)

ACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAGATTTGACGCCTGTAAAATTTCTTCG  >  NC_000913/1193701‑1193962                                                                                                                                        |                                                                                                                               aCAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTTCTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCt                                                                                                                            <  1:630623/140‑1 (MQ=255)   aGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCttt                                                                                                                          >  2:113106/1‑140 (MQ=255)           aGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAgg                                                                                                                  <  1:396952/140‑1 (MQ=255)               aCGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAg                                                                                                              >  2:149562/1‑140 (MQ=255)                       aTCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATc                                                                                                      <  1:113106/140‑1 (MQ=255)                        tCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCg                                                                                                     <  1:899814/140‑1 (MQ=255)                             acCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAat                                                                                                >  1:685861/1‑140 (MQ=255)                                   ggACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCAtctc                                                                                          <  2:166094/140‑1 (MQ=255)                                         gCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCgg                                                                                    >  2:833603/1‑140 (MQ=255)                                                     tttttttGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGccc                                                                        <  1:166615/140‑1 (MQ=255)                                                        ttttGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCt                                                                     <  1:149562/140‑1 (MQ=255)                                                              ggTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATa                                                               <  1:251121/140‑1 (MQ=255)                                                                 ttCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATaaca                                                            >  1:517090/1‑140 (MQ=255)                                                                          tGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATa                                                               <  1:479611/128‑1 (MQ=255)                                                                          tGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATa                                                               >  2:479611/1‑128 (MQ=255)                                                                            gATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACttt                                                 >  2:471557/1‑140 (MQ=255)                                                                            gATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACttt                                                 >  2:932379/1‑140 (MQ=255)                                                                                  tcacGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGcca                                           >  2:507266/1‑140 (MQ=255)                                                                                  tcacGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGcca                                           >  2:601250/1‑140 (MQ=255)                                                                                    acGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCCGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGccacc                                         >  1:91416/1‑140 (MQ=255)                                                                                             aaCTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGcgaa                                <  1:509882/140‑1 (MQ=255)                                                                                                  cAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAg                           >  2:275614/1‑140 (MQ=255)                                                                                                            tATTCTATCACGCAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAGATTTGACGcc                 <  1:932379/140‑1 (MQ=255)                                                                                                                        cAGCCTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAGATTTGACGCCTGTAAAATttct     >  1:858116/1‑140 (MQ=255)                                                                                                                           ccTTTTAGATGACCCCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAGATTTGACGCCTGTAAAATTTCTTCg  >  1:490067/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                               ACAGCCAGGCAGAAACGGAGGAATCGACACCGCCGGACATGCCGACGATTACTTTTTTTGCGGTTTCAGACATTGGATCACTCACGACATTGAACTTCAAGGCGGCGTATTCTATCACGCAGCCTTTTAGATGACACCCTTTGTAAAAGGCCAGTTAAAATCGCCAATCATCTCCAGCGGATAACGTTGCCCGCTTTGATAACAACGAATACTTTCCGCCACCAGCGGCGAACGAAGATTTGACGCCTGTAAAATTTCTTCG  >  NC_000913/1193701‑1193962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑