Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,947,343	(A)8→7	intergenic (‑165/‑44)	mltA ← / → metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,947,336	0	A	.	100.0%	214.1 / NA	50	intergenic (‑158/‑51)	mltA/metZ	membrane‑bound lytic murein transglycosylase A/tRNA‑Met
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (19/31);  total (19/31)

AGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT  >  NC_000913/2947205‑2947465                                                                                                                                     |                                                                                                                                  aGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGTaaaaaaagg                                                                                                                           <  2:1071336/140‑3 (MQ=255)  ggCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGTaaaaaaaggt                                                                                                                          <  1:831371/140‑4 (MQ=255)    cgcAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTg                                                                                                                       >  2:397345/1‑140 (MQ=255)    cgcAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTg                                                                                                                       >  1:402083/1‑140 (MQ=255)               tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                            <  1:750739/140‑1 (MQ=255)               tAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                            <  2:929955/140‑1 (MQ=255)               aaaCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                            <  2:34791/139‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                          >  1:1142348/1‑140 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                          <  1:155381/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                          <  2:719470/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                          <  2:1142348/140‑1 (MQ=255)                 aCAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGa                                                                                                          <  1:681564/140‑1 (MQ=255)                  cAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAg                                                                                                         <  2:1054629/140‑1 (MQ=255)                   aaaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGc                                                                                                        <  1:397345/140‑1 (MQ=255)                    aaCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCg                                                                                                       <  1:1071353/140‑1 (MQ=255)                     aCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCTGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCgg                                                                                                      >  2:33768/1‑140 (MQ=255)                     aCCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCgg                                                                                                      >  2:328781/1‑140 (MQ=255)                      ccgccgCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGa                                                                                                     <  2:315801/140‑1 (MQ=255)                        gccgcGTTTAATTAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                            <  2:493821/131‑1 (MQ=255)                        gccgcGTTTAATTAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAAcgc                                                                                                            >  1:493821/1‑131 (MQ=255)                            cgTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAg                                                                                               >  1:991959/1‑140 (MQ=255)                                      aatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                     <  1:1008662/140‑1 (MQ=255)                                      aatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                     <  2:547095/140‑1 (MQ=255)                                      aatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                     <  2:1093362/140‑1 (MQ=255)                                      aatgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCa                                                                                     <  2:529848/140‑1 (MQ=255)                                        tgaGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCAc                                                                                    >  1:1101395/1‑140 (MQ=255)                                            cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                               <  2:555858/140‑1 (MQ=255)                                            cagcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                               <  1:750444/140‑1 (MQ=255)                                             agcCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAATTCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGAcg                                                                               <  2:1101395/140‑1 (MQ=255)                                                      cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                                     <  2:1029262/140‑1 (MQ=255)                                                      cTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGag                                                                     <  2:82185/140‑1 (MQ=255)                                                      cTGACGCAAATTTTACACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCACATCCACGGACGCGGGGTGGag                                                                     <  1:860561/140‑1 (MQ=255)                                                         aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCggg                                                                           <  1:355263/131‑1 (MQ=255)                                                         aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCggg                                                                           >  2:355263/1‑131 (MQ=255)                                                         aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagc                                                                    >  1:1047025/1‑138 (MQ=255)                                                         aCGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGagc                                                                    <  2:1047025/138‑1 (MQ=255)                                                                 tttGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAg                                                          <  2:243103/140‑1 (MQ=255)                                                                                  cTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCAACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAAc                                         >  2:412052/1‑140 (MQ=255)                                                                                     tAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCg                                      >  1:802829/1‑140 (MQ=255)                                                                                           tttgtttttgttCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                >  2:245238/1‑140 (MQ=255)                                                                                           tttgtttttgttCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtc                                >  2:864684/1‑140 (MQ=255)                                                                                             tgtttttgttCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                              <  2:802829/140‑1 (MQ=255)                                                                                             tgtttttgttCAAAA‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                              <  2:991959/140‑1 (MQ=255)                                                                                                         aaa‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATcc                  >  1:824831/1‑140 (MQ=255)                                                                                                          aa‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg                 <  1:864684/140‑1 (MQ=255)                                                                                                          aa‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg                 >  1:562540/1‑140 (MQ=255)                                                                                                          aa‑TCATGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCg                 <  1:245238/140‑1 (MQ=255)                                                                                                                tGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAACCGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGGGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGcccc            >  1:843249/1‑140 (MQ=255)                                                                                                                tGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                              <  2:1113609/122‑1 (MQ=255)                                                                                                                tGCCAAATCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGgtcgt                              >  1:1113609/1‑122 (MQ=255)                                                                                                                      aTCCGTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAAc      >  1:626057/1‑140 (MQ=255)                                                                                                                          gTGATCGGGGT‑AAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAt  <  1:1140965/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                  AGGCGCAACGAAGATAACAAACCGCCGCGTTTAATGAAATGAGCAGCCTCTCCCTGACGCAAATTTTGCACAAAAAATAGGCTTTAGTGATTTGTTTTTGTTCAAAA‑TCATGCCAAATCCGTGATCGGGGTAAAAAAAAGGTTGCATGAAAACGCGAGCGGAGTATAGTGCGCATCCACGGACGCGGGGTGGAGCAGCCTGGTAGCTCGTCGGGCTCATAACCCGAAGGTCGTCGGTTCAAATCCGGCCCCCGCAACCAAT  >  NC_000913/2947205‑2947465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑