| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 22.7% | 73.7 / 18.1 | 44 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (2/32); new base G (10/0); total (12/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.66e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646611‑646886 | tttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGTATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCg < 2:52659/140‑1 (MQ=255)tttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCg < 1:577646/140‑1 (MQ=255) ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGa > 1:721833/1‑140 (MQ=255) aTCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAAt > 2:1116456/1‑140 (MQ=255) cAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑c < 2:570402/140‑1 (MQ=255) gTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑cccc < 1:744137/140‑1 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCt < 1:257568/140‑1 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATG‑CCCCCtt > 1:630769/1‑140 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCttt < 2:1064804/140‑1 (MQ=255) agacagatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCtttt < 1:256128/134‑1 (MQ=255) aataatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTg < 1:10525/140‑1 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGAAG‑CCCCCCTTTTTCCATAATTTTTgcc > 1:57827/1‑139 (MQ=255) tgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTGCAATAATATTTtcg > 1:737346/1‑140 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGGTG‑CCCCCCTTTTTTTATAATGttttttt > 2:256128/1‑136 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTc < 1:1148324/140‑1 (MQ=255) aaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGACCCCCTTTTTATAAAACTGtttcggctcc > 1:719211/1‑133 (MQ=255) gATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAgc < 1:863633/140‑1 (MQ=255) gATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTTAATAAAGTTTTCGCCCCCCGCTGTAGCCaag > 1:45821/1‑138 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAgg < 2:718665/140‑1 (MQ=255) tAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTTCATAAATTTTTCGCCCCGCGCCGTAGCCAGCGCCGGc > 1:1165947/1‑140 (MQ=255) gTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTCAATAATTTTTTCGCCTCCCGCCGGAGCCCGCGCAGGcccc > 2:132874/1‑140 (MQ=255) tCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGccccc < 2:388748/140‑1 (MQ=255) aGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCt < 1:1155591/140‑1 (MQ=255) cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTTGAATG‑GCCCCTTTTTTTTAAAATGTTTTTGCCCCTCGCGGTGGCCTGCGCCGGcgccccccc > 2:908470/1‑137 (MQ=255) cACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTTAATAATATTTTCGCCTCCCGCCGTAGCCAGCGCCGGccccccccc > 2:408708/1‑137 (MQ=255) aCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGc < 2:648921/140‑1 (MQ=255) ccgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTTAATAATGTTTTCGCCCCGCGCCTGAGCCAGCGCAGGCCCCCCCCCCCAcc > 1:359568/1‑140 (MQ=255) gccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgc < 1:814122/140‑1 (MQ=255) aCTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATAGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATg < 2:1104504/140‑1 (MQ=255) cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc < 1:177085/140‑1 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGa < 2:630769/140‑1 (MQ=255) tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt < 2:721833/140‑1 (MQ=255) tCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGAt < 2:214366/140‑1 (MQ=255) tGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGcc < 1:119743/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTCAATAATGTTTTCGCCCCCCGCCGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCGCGGTGCAACGTTGGTATCGCCGCTGATCGccc > 2:604308/1‑139 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 1:1061876/140‑1 (MQ=255) tCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 1:1116456/140‑1 (MQ=255) cGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATG‑CCCCCCTTTTTCAATAATTTTTGCGCCTCGCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCCACCTTGGTATCGCCGTTGATCGCCCTc > 2:1061876/1‑140 (MQ=255) cGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑GCCCCCTTTTTCAATAATGTTTTTGCCCCCCGCTGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGGGGCAACGTTGGGTTTGCCGGTGTTCGccccc > 2:500519/1‑137 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACGGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 2:870351/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 2:45821/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 1:285707/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGGTTGAATG‑CCCCCCTTTTTCAATAATTTTTTCGCCCCCCCCCGTTGCCCGCGCCGGCCCCCCCCCCCCCCCCCCCGCGCCACGCTTGTTTCCCCGTTGGTCGCCCTCCGTAg > 2:1073424/1‑140 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATG‑CCCCCCTTTTTCAATAATGTTTTCGCCTCGCGCTGGAGGCCGCGCCGGCCCCCCCCCCCCCCCCCCCGTGCAACGTTGGTATCGCCCTTGGTCGCCCTCAGGAg > 2:146476/1‑140 (MQ=255) gCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAgg < 1:408708/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:156732/140‑1 (MQ=255) aTCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt < 1:200386/140‑1 (MQ=255) gcggGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 1:362937/140‑1 (MQ=255) gcggGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 2:719211/140‑1 (MQ=255) ggTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGtt < 2:737346/140‑1 (MQ=255) ttCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa < 1:295706/140‑1 (MQ=255) | TTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATG‑CCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646611‑646886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |