Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT32.1% 36.2 / 14.3 28intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (2/17);  new base T (9/0);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 7.96e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG  >  NC_000913/3206298‑3206561
                                                                                                                                          |                                                                                                                               
aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCa                                                                                                                               >  2:157842/1‑140 (MQ=255)
    cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGTAATTTTTcaaaaa                                                                                                                            >  1:758910/1‑135 (MQ=255)
                   ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGa                                                                                                            >  2:810594/1‑140 (MQ=255)
                   ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTTAGACAaaaaaaaaaaaagga                                                                                                            >  2:25728/1‑135 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGGTgg                                                                                                       >  2:906544/1‑139 (MQ=255)
                          gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCa                                                                                                     <  1:751175/140‑1 (MQ=255)
                                    ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                           <  1:1108083/140‑1 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGGGaaaaa                                                                                       >  1:345070/1‑138 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAggggaaaa                                                                                       >  2:664874/1‑133 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                    <  1:664874/140‑1 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                    <  1:810594/140‑1 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                    <  1:932163/140‑1 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                    <  2:1183529/140‑1 (MQ=255)
                                             tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTT‑GATTTTTTCCGACAAATAAAAAAAATCGAGTTGGCATGGTGAGGGAAAGtaaaaa                                                                                  >  1:759587/1‑140 (MQ=255)
                                               gcgTTGATTCACGAACTGTTAATTACATATTCACGGTTGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGTGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc                                                                                >  1:1122183/1‑140 (MQ=255)
                                                 gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                              <  2:303951/140‑1 (MQ=255)
                                                  ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAGAAATAAACaaa                                                                             >  2:678779/1‑139 (MQ=255)
                                                     aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                          <  1:678779/140‑1 (MQ=255)
                                                      ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                         <  2:1133223/140‑1 (MQ=255)
                                                              cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATa                                                                <  1:157842/140‑1 (MQ=255)
                                                                    aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAAACAAAAACAAAAAATGGGGTTGCCATGATGAGGGAAAAGAAAAAAAAAAAAGCAG‑TGGATAAgatta                                                          >  2:974697/1‑136 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAATAAAAAAAAAGGAGGTGCCATGATGAGCGGAAGGAAAAAACAAAAAACAG‑AGAAAAAAATGACAgagaa                                                   >  2:1106776/1‑139 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATAGAGGTTGCATGATGAGGGAAAGTAAAAAACAAAAAGCAG‑AGAATAAAATGACAgagaa                                                   >  2:870587/1‑139 (MQ=255)
                                                                                 ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAATGGAGTTGGCATGATGAGCGAAAGGAAAAAACAAAAAGCAA‑GGAAAAAATTGACAGAGAATGTCGc                                             >  2:804467/1‑140 (MQ=255)
                                                                                      cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGACAAAAAAAAAAAATGGAGTTGGCATGATGGGGGAAAATAAAAAACAAAAAGCAGAGGAAAAAGTTG‑CAGAGAATGTTGCTaaat                                        >  2:1005455/1‑138 (MQ=255)
                                                                                         aagatatAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCAAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                     <  1:71640/137‑1 (MQ=255)
                                                                                           aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                   <  1:870587/140‑1 (MQ=255)
                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTAAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGGAAATAAAAAAAAACAAGCAG‑TGAAAAAATTTGCAAAGATTGTCGCTAAATTTACCCCCCTGAttttt                       >  2:71640/1‑138 (MQ=255)
                                                                                                       caTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                       <  1:974697/140‑1 (MQ=255)
                                                                                                            ttAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg                  <  2:447202/140‑1 (MQ=255)
                                                                                                             tAGTGGTAGTTTGTGGTGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                 <  2:1122183/140‑1 (MQ=255)
                                                                                                              aGTGGTAGTTTGTGGGGGTA‑ATTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGaa                <  2:345070/140‑1 (MQ=255)
                                                                                                                      tttGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgat        <  1:804467/140‑1 (MQ=255)
                                                                                                                            gCGGTA‑ATTTTTTCGAGAAAAATAAAAAGTCTGGAGTTGCCATGAGGAGGAAAAGAAATAACCACCGGGCAG‑TGAATAAGTTGACAGAGATTGTCGCAAGTTTAACCGCCATGATTTCTACCCGAATGCCTGATGACgtg  <  2:426020/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                               
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAG‑TGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTG  >  NC_000913/3206298‑3206561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑