Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,404,218	0	T	G	26.8%	96.7 / 16.5	56	G245G (GGT→GGG)	yhdH	acrylyl‑CoA reductase
Reads supporting (aligned to +/- strand):  ref base T (5/36);  new base G (15/0);  total (20/36)
Fisher's exact test for biased strand distribution p-value = 9.54e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT  >  NC_000913/3404084‑3404352                                                                                                                                       |                                                                                                                                       tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGtttttt                                                                                                                                   >  2:633145/1‑138 (MQ=255) tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGgttttt                                                                                                                                   >  2:985519/1‑138 (MQ=255) tttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTAc                                                                                                                                   >  1:323824/1‑140 (MQ=255)  ttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAct                                                                                                                                  <  2:11587/140‑1 (MQ=255)  ttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAct                                                                                                                                  <  2:916558/140‑1 (MQ=255)  ttGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTAct                                                                                                                                  <  2:136477/140‑1 (MQ=255)     ccGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTg                                                                                                                               <  1:56386/140‑1 (MQ=255)         aTCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGccca                                                                                                                           >  2:313478/1‑138 (MQ=255)               tCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACgg                                                                                                                     >  1:796287/1‑140 (MQ=255)                 ctctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTc                                                                                                                   <  1:242825/140‑1 (MQ=255)                   ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  1:633145/140‑1 (MQ=255)                   ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  1:605255/140‑1 (MQ=255)                   ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  1:665495/140‑1 (MQ=255)                   ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  1:898419/140‑1 (MQ=255)                   ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  1:21477/140‑1 (MQ=255)                   ctGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCAt                                                                                                                 <  1:198883/140‑1 (MQ=255)                      gAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACGGTCATGcc                                                                                                              >  1:1015759/1‑140 (MQ=255)                           aCAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCCGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCATGCCCtttt                                                                                                         >  2:242825/1‑139 (MQ=255)                            cAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCCACCACGGGCCTGCCCttttt                                                                                                        >  2:219389/1‑138 (MQ=255)                             aaGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTAtt                                                                                                       <  2:782333/140‑1 (MQ=255)                              aGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCGTGCCCTTTAttt                                                                                                      >  2:21477/1‑139 (MQ=255)                                tCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTg                                                                                                    <  1:619146/140‑1 (MQ=255)                                  tgggctgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCg                                                                                                  <  1:730233/140‑1 (MQ=255)                                       tgggGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCATTTTTTTTTCGGAATg                                                                                             >  2:989909/1‑140 (MQ=255)                                          ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCATGCCATTTTTTTTGCGGAATGTcc                                                                                          >  2:305400/1‑140 (MQ=255)                                          ggCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGTCATGCCCTTTTTTCTGCGGAATGTcc                                                                                          >  2:71904/1‑140 (MQ=255)                                           gCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTGTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCg                                                                                         <  1:577506/140‑1 (MQ=255)                                              aTTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCCGTGGTCTGGCGGGGGGGTTTTTTCTGTCCAACACAGGCATGGCATTTATTTTTTGTTATGTTCGttt                                                                                      >  2:284014/1‑140 (MQ=255)                                                tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGc                                                                                    <  1:773410/140‑1 (MQ=255)                                                tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGc                                                                                    <  1:884230/140‑1 (MQ=255)                                                tGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGc                                                                                    <  2:1184345/140‑1 (MQ=255)                                                 gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa                                                                                   <  2:796287/140‑1 (MQ=255)                                                 gACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCa                                                                                   <  2:677853/140‑1 (MQ=255)                                                  acacCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCaa                                                                                  <  1:793414/140‑1 (MQ=255)                                                       gTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                             <  1:989909/140‑1 (MQ=255)                                                         tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCCCGGGCATGCCATTTATTTTTCGTAATGTCCGGTTTCCAGGGGTgg                                                                           >  2:393933/1‑140 (MQ=255)                                                         tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGAGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                             >  2:687199/1‑138 (MQ=255)                                                         tGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGAGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGt                                                                             <  1:687199/138‑1 (MQ=255)                                                          ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGa                                                                          <  2:627040/140‑1 (MQ=255)                                                          ggCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTTTCTTCCCACCACGGGCACGCCAATTTTTCTGCGTAATATCCGTTTTCAAGggggggg                                                                          >  2:805019/1‑139 (MQ=255)                                                                  aGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGGTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTaa                                                                  >  1:97898/1‑140 (MQ=255)                                                                  aGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGGGGATTCAGTaa                                                                  >  2:136266/1‑140 (MQ=255)                                                                      cTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGAc                                                              <  1:456097/140‑1 (MQ=255)                                                                        ggCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGc                                                            <  1:985519/140‑1 (MQ=255)                                                                              aGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCCACCCCGGTCATTCCCTTTTTTTTGCGTAATGTCCGTTTTCAAGGGGGGGGTTCAGTAATGACGccaccca                                                      >  2:895959/1‑138 (MQ=255)                                                                                tGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCCCGGGCATGCCCTTTTTTCTGCGTAATGTTCGTTTGCAAGGGGGGGGTTCAGGAATGACGCCCCCCGaa                                                    >  2:456097/1‑140 (MQ=255)                                                                                  cTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcg                                                  <  1:101047/140‑1 (MQ=255)                                                                                  cTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcg                                                  <  1:793794/140‑1 (MQ=255)                                                                                  cTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAAcg                                                  <  1:726279/140‑1 (MQ=255)                                                                                     gcgcAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACCGGCATGCCATTTATTTTGGGGAATGTCCGGTTTCAAGGGGGGGGTTCAGTAATGACGCCACCAGAAcgccc                                               >  2:675240/1‑139 (MQ=255)                                                                                        cAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCgcgc                                            <  1:895959/140‑1 (MQ=255)                                                                                          aaTGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGcac                                          <  1:1076253/140‑1 (MQ=255)                                                                                                     gcggcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTGCCAACCACGGGCATGCCCTTTTTTTTTCGTAATGTCCGTTTGCAAGGGGGGGGTTCAGTAATGACGCCACCAGAAAGCCGCGCCCAAACCTGGGAg                               >  2:1117554/1‑140 (MQ=255)                                                                                                        gcTGCGTGGCGGCCTGTGGTCTGGCGGGGGGGTTTTCTCTTCCAACCACGGGCATGCCATTTATTTTGCGGAATGTCCGTTTGCAAGGGGGGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAACCCGGCAGCgg                            >  2:431080/1‑139 (MQ=255)                                                                                                              tGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTc                      >  1:290959/1‑140 (MQ=255)                                                                                                                  ggcCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCg                  >  2:1148534/1‑140 (MQ=255)                                                                                                                             tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc            >  2:542657/1‑135 (MQ=255)                                                                                                                             tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc            <  1:542657/135‑1 (MQ=255)                                                                                                                             tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa       <  1:219389/140‑1 (MQ=255)                                                                                                                             tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa       <  1:1148534/140‑1 (MQ=255)                                                                                                                             tGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGaa       <  1:1117554/140‑1 (MQ=255)                                                                                                                              ggCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAAt      <  2:1015759/140‑1 (MQ=255)                                                                                                                                cGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCa    >  1:612868/1‑140 (MQ=255)                                                                                                                                  ggtggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCAtt  >  1:286134/1‑140 (MQ=255)                                                                                                                                    tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc            >  2:844511/1‑128 (MQ=255)                                                                                                                                    tggtTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTAc            <  1:844511/128‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TTTGCCGAATCCCGTCCTCTGGAAAAACAAGTCTGGGCTGGGGCAATTGACACCGTTGGCGACAAAGTGCTGGCAAAAGTGCTGGCGCAAATGAATTACGGCGGCTGCGTGGCGGCCTGTGGTCTGGCGGGTGGTTTTACTCTGCCAACCACGGTCATGCCATTTATTCTGCGTAATGTCCGTTTGCAAGGGGTGGATTCAGTAATGACGCCACCAGAACGCCGCGCACAAGCCTGGCAGCGACTGGTCGCCGATTTACCGGAATCATT  >  NC_000913/3404084‑3404352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑