Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,170,111	G→C	pseudogene (57/475 nt)	gatR ←	DNA‑binding transcriptional repressor GatR, N‑terminal fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,170,111	0	G	C	100.0%	237.9 / NA	61	pseudogene (57/475 nt)	gatR	DNA‑binding transcriptional repressor GatR, N‑terminal fragment
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (27/34);  total (27/34)

GCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAA  >  NC_000913/2169977‑2170245                                                                                                                                       |                                                                                                                                       gCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTc                                                                                                                                   >  2:665721/1‑140 (MQ=255)   tCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCg                                                                                                                                      >  1:665698/1‑135 (MQ=255)   tCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCg                                                                                                                                      >  2:120328/1‑135 (MQ=255)   tCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCAt                                                                                                                                 >  2:545552/1‑140 (MQ=255)   tCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCAt                                                                                                                                 >  1:16245/1‑140 (MQ=255)   tCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCCAGCCACCACAGGCCACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTTTTGGTAATCACCGGGATAATTTTGCCGGTCAt                                                                                                                                 >  2:741969/1‑140 (MQ=255)      gCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTaa                                                                                                                              <  2:431248/140‑1 (MQ=255)            gAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTc                                                                                                                        >  2:686885/1‑140 (MQ=255)             aaCCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCg                                                                                                                       <  1:784688/140‑1 (MQ=255)             aaCCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCATGATATTTTTGCCGGTCATTAATCCTTCg                                                                                                                       >  2:655646/1‑140 (MQ=255)                  tGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGAt                                                                                                                  >  2:146434/1‑140 (MQ=255)                  tGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGAt                                                                                                                  >  2:73568/1‑140 (MQ=255)                  tGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGAt                                                                                                                  >  2:738983/1‑140 (MQ=255)                  tGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGAt                                                                                                                  <  1:94723/140‑1 (MQ=255)                         aGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCAtt                                                                                                           >  2:510533/1‑140 (MQ=255)                           cgcgTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGt                                                                                                         <  1:665721/140‑1 (MQ=255)                                     ggCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGTAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCg                                                                                               <  1:240527/140‑1 (MQ=255)                                     ggCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCg                                                                                               <  1:1085319/140‑1 (MQ=255)                                        ggACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTg                                                                                            <  1:1043256/140‑1 (MQ=255)                                          aCCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCTTTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTc                                                                                          >  2:350998/1‑140 (MQ=255)                                                 caccacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATa                                                                                   <  1:686885/140‑1 (MQ=255)                                                 caccacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTCGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATa                                                                                   <  2:918857/140‑1 (MQ=255)                                                  accacAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATaa                                                                                  <  2:16245/140‑1 (MQ=255)                                                      caGACCGCCAGAGTAATGTATTTATTTTCGCAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGCTCTTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGt                                                                              <  2:552863/140‑1 (MQ=255)                                                        gacgacCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCt                                                                            >  1:352402/1‑140 (MQ=255)                                                               agagTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCTTTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTa                                                                     <  1:350998/140‑1 (MQ=255)                                                               agagTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTa                                                                     <  1:510533/140‑1 (MQ=255)                                                                 agTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACgg                                                                               <  1:981124/128‑1 (MQ=255)                                                                 agTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACgg                                                                               >  2:981124/1‑128 (MQ=255)                                                                 agTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTAcc                                                                   >  2:325439/1‑140 (MQ=255)                                                                 agTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCATGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTAcc                                                                   <  1:655646/140‑1 (MQ=255)                                                                    aaTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAg                                                                <  1:545552/140‑1 (MQ=255)                                                                    aaTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAg                                                                <  1:738983/140‑1 (MQ=255)                                                                     aTGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGc                                                               >  2:852266/1‑140 (MQ=255)                                                                       gTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCaa                                                             >  2:1015821/1‑140 (MQ=255)                                                                           ttATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGt                                                         >  2:186158/1‑140 (MQ=255)                                                                               tttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGac                                                     <  1:795705/140‑1 (MQ=255)                                                                                ttCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGaca                                                    <  1:1009181/140‑1 (MQ=255)                                                                                   ggAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGac                                                     >  2:228093/1‑136 (MQ=255)                                                                                   ggAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGac                                                     <  1:228093/136‑1 (MQ=255)                                                                                       aGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGACt                                             <  2:588998/140‑1 (MQ=255)                                                                                        gggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTa                                            <  1:673632/140‑1 (MQ=255)                                                                                         ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTaa                                           >  2:564712/1‑140 (MQ=255)                                                                                         ggCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTaa                                           <  1:1015821/140‑1 (MQ=255)                                                                                                  tGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTTATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTaaa                                  <  1:146434/140‑1 (MQ=255)                                                                                                     cGGGAGACTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAActc                               <  2:352402/140‑1 (MQ=255)                                                                                                          gaCTGTTGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCtcagt                          <  1:564712/140‑1 (MQ=255)                                                                                                               ttGGTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGGAAACTCTCAGTCagag                     >  1:502500/1‑140 (MQ=255)                                                                                                                 ggTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTaa                                   <  2:1103306/124‑1 (MQ=255)                                                                                                                 ggTAATCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTaa                                   >  1:1103306/1‑124 (MQ=255)                                                                                                                     aTCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACt               <  1:852266/140‑1 (MQ=255)                                                                                                                     aTCACCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACt               >  2:538839/1‑140 (MQ=255)                                                                                                                        aCCGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTcac            <  1:538839/140‑1 (MQ=255)                                                                                                                         ccGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTcaca           >  1:621401/1‑140 (MQ=255)                                                                                                                         ccGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTcaca           >  1:112051/1‑140 (MQ=255)                                                                                                                         ccGTGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCCGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTcaca           >  2:1071764/1‑140 (MQ=255)                                                                                                                            tGATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGa        >  2:847259/1‑140 (MQ=255)                                                                                                                             gATATTTTTGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGAc       <  2:333653/140‑1 (MQ=255)                                                                                                                                  ttttGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaaaaa  <  2:621401/140‑1 (MQ=255)                                                                                                                                  ttttGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaaaaa  <  1:325439/140‑1 (MQ=255)                                                                                                                                  ttttGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaaaaa  <  1:1049921/140‑1 (MQ=255)                                                                                                                                  ttttGCCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCCGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACaaaaa  <  1:1071764/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCTCGGCAATGGAACCATGCATCGAGCGCGTTTTATGGCGGACCGTGCCACCACAGACGACCAGAGTAATGTCTTTATTTTCGGAAAGGGCAAACGCTGCCGGGAGACTGTTGGTAATCACCGTGATATTTTTGGCGGTCATTAATCCTTCGGCGATAAGCATTGTCGTACTTCCGCTGTCGAGAATAACGGTCTGATCTTACCCAGCAATAGTGGACACGCGGCTAAGTGAGTAAACTCTCAGTCAGAGGTGACTCACATGACAAAAA  >  NC_000913/2169977‑2170245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑