Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,1140GT25.6% 116.8 / 11.1 43intergenic (‑319/+71)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (25/7);  new base T (0/11);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 5.53e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGC  >  NC_000913/752977‑753250
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aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGtt                                                                                                                                        <  2:1467046/140‑1 (MQ=255)
aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGtt                                                                                                                                        >  2:1274595/1‑140 (MQ=255)
aTTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGtt                                                                                                                                        <  1:943946/140‑1 (MQ=255)
                    gctaCTACCATGAATTTTTTAATTAAAAGTATTGGGTTGCTAATAATTTGAGCTTTTTTATTTTTTTAAAATATTTATATAGGTCTGTTAATGGTTTTTATTTTTAAAATTTTTTGTGTTTAGGCATAAAAAAATCAAcc                                                                                                                    <  2:921233/137‑1 (MQ=255)
                    gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAcc                                                                                                                    >  1:1022421/1‑140 (MQ=255)
                    gCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATAAAcc                                                                                                                    >  1:1529618/1‑140 (MQ=255)
                     cGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAAccc                                                                                                                   >  2:1433253/1‑140 (MQ=255)
                       aCTACAATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTTTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGAATAAAAAAATCAACCCGc                                                                                                                 <  1:1507823/140‑1 (MQ=255)
                                  ttttttAATTAAAATTTTTGGTTTGCTGATAATTTGAGCTTTTCTTTTTTTTTAAAATTTTTATATAGGTCTTTAAATGGTTTTTATTTTTACAATTTTTTGTTTTTAGAAATAAAAAAACAAACCCGCCATATGAAcgg                                                                                                      <  2:1022421/139‑1 (MQ=255)
                                           taaaaGTATTGGTTTGCTAATATTTTGAGTTTTTTTTTTTTTTTAAAATATCTATATAGTTTTTTTAATGTTTTTTTTTTTTACATTTTTTTTTTTTTAGCAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTaaa                                                                                             <  2:1047740/138‑1 (MQ=255)
                                                           cTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGc                                                                             >  1:1487151/1‑140 (MQ=255)
                                                               tAATTTGAGTTTTTCTATTTTTTTTAAATATCTATATAGGTCTGTTAATGGTTTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATc                                                                         <  1:463288/140‑1 (MQ=255)
                                                                     gAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCAt                                                                   >  1:829729/1‑140 (MQ=255)
                                                                           ttCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGc                                                             >  1:842062/1‑140 (MQ=255)
                                                                                   tttttAAATATCTATATAGGTCTGTTAATGGTTTTTATTTTTACATTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATc                                                     <  1:1238443/140‑1 (MQ=255)
                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTTTAACTTAGCAATCAACCATTAACGCTTGATATTGc                                                   >  2:80037/1‑140 (MQ=255)
                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                   >  1:932093/1‑140 (MQ=255)
                                                                                     tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAAACAACCATTAACGCTTGATATCGc                                                   >  2:83843/1‑140 (MQ=255)
                                                                                                      gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTTAAGTCGCGtttt                                  >  1:740903/1‑140 (MQ=255)
                                                                                                      gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                  >  2:660204/1‑140 (MQ=255)
                                                                                                       tCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGttttt                                 >  1:519269/1‑140 (MQ=255)
                                                                                                       tCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGgtttt                                 >  2:752935/1‑140 (MQ=255)
                                                                                                         tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                               >  1:893623/1‑140 (MQ=255)
                                                                                                         tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                               >  2:514563/1‑140 (MQ=255)
                                                                                                         tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                               >  1:195788/1‑140 (MQ=255)
                                                                                                          tttAATGTTTTTTTTTTTTAAATTTTTTTGTTTTAAGAAATAAAAAAACCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  2:80447/139‑1 (MQ=255)
                                                                                                          tttAATGTTTTTTATTTTTATATTTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  2:724087/139‑1 (MQ=255)
                                                                                                          tttAATGTTTTTTATTTTTACATTTTTTTGTTTTTAGGAAAAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  2:1193894/139‑1 (MQ=255)
                                                                                                          tttAATGGTTTTTTTTTTTACAATTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  1:1433253/139‑1 (MQ=255)
                                                                                                          tttAATGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  2:519269/139‑1 (MQ=255)
                                                                                                          tttAATGGTTTTTATTTTTAAATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  2:952671/139‑1 (MQ=255)
                                                                                                          gTTAATGGTTTTTTTTTTTAAATTTTTTTGTTTTTAGAAATAAAAAAAACAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  2:842062/140‑1 (MQ=255)
                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                             >  2:1207574/1‑140 (MQ=255)
                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                             >  2:1008143/1‑140 (MQ=255)
                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                             >  1:272120/1‑140 (MQ=255)
                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                             >  1:221043/1‑140 (MQ=255)
                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                             >  1:1193894/1‑140 (MQ=255)
                                                                                                           ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAAAGCTTGATATCGCTTTTAAAATCGCGTTTTTCata                             >  2:736530/1‑140 (MQ=255)
                                                                                                             aatgatttttttttttAAATTTTTTTGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                           <  1:566409/134‑1 (MQ=255)
                                                                                                             aaTGGTTTTTTTTTTTACAATTTTTTGTGTTTAGGCATAAAAAAAACACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                           <  2:220972/140‑1 (MQ=255)
                                                                                                                ggATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTg                        >  1:80447/1‑140 (MQ=255)
                                                                                                                   ttttttttttAAATTTTTTTGTTTTTAGGAATAAAAAAACCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGtat                     <  2:829729/140‑1 (MQ=255)
                                                                                                                     ttattttTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              >  2:621181/1‑129 (MQ=255)
                                                                                                                     ttattttTACAATTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                              <  1:621181/129‑1 (MQ=255)
                                                                                                                       ttttttAAATTTTTTTGTTTTTAGGAATAAAAAAATCACCCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAg                 <  2:704755/139‑1 (MQ=255)
                                                                                                                                      tgtgTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc  <  1:1008143/140‑1 (MQ=255)
                                                                                                                                      tgtgTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGc  <  1:1274595/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ATTTTCATAGGGTTATAAATGCGACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGC  >  NC_000913/752977‑753250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑