Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,746,186	A→T	intergenic (‑1/+248)	rpsP ← / ← ffh	30S ribosomal subunit protein S16/signal recognition particle protein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,746,186	0	A	T	100.0%	198.8 / NA	56	intergenic (‑1/+248)	rpsP/ffh	30S ribosomal subunit protein S16/signal recognition particle protein component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (29/27);  total (29/27)

TTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTG  >  NC_000913/2746049‑2746307                                                                                                                                          |                                                                                                                          ttttCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTAc                                                                                                                         <  2:668416/140‑1 (MQ=255)    tCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATc                                                                                                                      <  1:1148053/140‑1 (MQ=255)          gCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTtg                                                                                                                >  2:358071/1‑140 (MQ=255)          gCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTtg                                                                                                                >  2:290118/1‑140 (MQ=255)           cGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTtgt                                                                                                               <  1:652655/140‑1 (MQ=255)           cGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTtgt                                                                                                               <  2:718373/140‑1 (MQ=255)                   ttGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATaa                                                                                                       <  2:690161/140‑1 (MQ=255)                     gaagaaACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATaaaa                                                                                                     <  1:1315675/140‑1 (MQ=255)                      aagaaACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAaac                                                                                                    >  2:798883/1‑140 (MQ=255)                        gaaACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAaacaa                                                                                                  >  2:227641/1‑140 (MQ=255)                        gaaACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAaacaa                                                                                                  <  1:227641/140‑1 (MQ=255)                           aCCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCg                                                                                               >  2:657160/1‑140 (MQ=255)                           aCCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCg                                                                                               >  1:548585/1‑140 (MQ=255)                             cAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGa                                                                                             <  1:972474/140‑1 (MQ=255)                                cgcgCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGAccc                                                                                          >  2:214138/1‑140 (MQ=255)                                cgcgCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGAccc                                                                                          <  1:1293641/140‑1 (MQ=255)                                                   gTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCg                                                                       <  1:257774/140‑1 (MQ=255)                                                   gTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCg                                                                       <  2:575253/140‑1 (MQ=255)                                                     tGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGgt                                                                     <  2:548585/140‑1 (MQ=255)                                                     tGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGgt                                                                     <  1:214138/140‑1 (MQ=255)                                                       cGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGgtgt                                                                   >  2:577196/1‑140 (MQ=255)                                                       cGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGgtgt                                                                   >  2:342770/1‑140 (MQ=255)                                                           cATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCata                                                               >  2:983743/1‑140 (MQ=255)                                                              tACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTa                                                            >  2:1453888/1‑140 (MQ=255)                                                                cGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTaaa                                                          >  1:25150/1‑140 (MQ=255)                                                                     gTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGccc                                                     >  2:1226100/1‑140 (MQ=255)                                                                                caacCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTAc                                          >  2:305737/1‑140 (MQ=255)                                                                                 aacCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACt                                         <  1:349228/140‑1 (MQ=255)                                                                                      ggTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCAttt                                    >  2:535097/1‑140 (MQ=255)                                                                                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                             >  1:491582/1‑140 (MQ=255)                                                                                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                             >  1:645191/1‑140 (MQ=255)                                                                                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                             <  1:1226100/140‑1 (MQ=255)                                                                                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                             >  2:657724/1‑140 (MQ=255)                                                                                             cGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCggg                             >  1:381972/1‑140 (MQ=255)                                                                                              ggACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGc                                <  2:1248501/136‑1 (MQ=255)                                                                                              ggACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGc                                >  1:1248501/1‑136 (MQ=255)                                                                                              ggACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGa                            >  1:1120275/1‑140 (MQ=255)                                                                                                 cGCTTTTTGGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAAtt                         >  1:1488261/1‑140 (MQ=255)                                                                                                 cGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAAtt                         >  1:345141/1‑140 (MQ=255)                                                                                                  gCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTg                        >  1:405022/1‑140 (MQ=255)                                                                                                     ttttAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCaa                     >  1:443236/1‑140 (MQ=255)                                                                                                      tttAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAAt                    <  1:1535149/140‑1 (MQ=255)                                                                                                      tttAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAAt                    <  2:443236/140‑1 (MQ=255)                                                                                                       ttAGCGCCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATc                   <  1:1480899/140‑1 (MQ=255)                                                                                                           cgcCGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACa               <  1:151706/140‑1 (MQ=255)                                                                                                              cGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGtt            >  1:1203774/1‑140 (MQ=255)                                                                                                              cGTGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGtt            >  1:248185/1‑140 (MQ=255)                                                                                                                tGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGc          >  1:704723/1‑140 (MQ=255)                                                                                                                tGACGTGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGc          >  2:683931/1‑140 (MQ=255)                                                                                                                     tGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAAct     <  2:345141/140‑1 (MQ=255)                                                                                                                     tGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAAct     <  2:1372739/140‑1 (MQ=255)                                                                                                                     tGCTAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAAct     <  1:683931/140‑1 (MQ=255)                                                                                                                        tAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTctg  <  1:1230444/140‑1 (MQ=255)                                                                                                                        tAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTctg  <  1:358071/140‑1 (MQ=255)                                                                                                                        tAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTctg  <  1:305737/140‑1 (MQ=255)                                                                                                                        tAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTctg  <  1:290118/140‑1 (MQ=255)                                                                                                                        tAAACGAATAGTTACCATTACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTctg  <  2:1120275/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                          TTTTCGCTAGCGATTGGGTTGAAGAAACCAACGCGCTCGATGAAGCGACCGTTGCGTGCATTACGGCTGTCAGCGACAACAACCTGGTAGAACGGACGCTTTTTAGCGCCGTGACGTGCTAAACGAATAGTTACCATAACATCCTCTTGTGTGAATAAAACAACCGGACCCCATCGAGGAACGGAGTCCGGTGTCATATTAAAAGCCCGAAAATTTTACTCATTTTTGCGGGAATTGCAATCAACAGTTGCTAACTCTG  >  NC_000913/2746049‑2746307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑