Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,716,442	T→A	I261F (ATC→TTC)	tyrS ←	tyrosine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,716,442	0	T	A	100.0%	285.6 / NA	81	I261F (ATC→TTC)	tyrS	tyrosine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (41/40);  total (41/40)

TGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCAAACACCTGATT  >  NC_000913/1716306‑1716571                                                                                                                                         |                                                                                                                                  tGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCa                                                                                                                                >  1:18927/1‑140 (MQ=255)  gCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAg                                                                                                                               <  1:1121238/140‑1 (MQ=255)  gCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAg                                                                                                                               <  1:1388459/140‑1 (MQ=255)  gCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAg                                                                                                                               <  2:911277/140‑1 (MQ=255)     ccGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAAc                                                                                                                            >  1:637337/1‑140 (MQ=255)      cGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACt                                                                                                                           >  1:126960/1‑140 (MQ=255)        ccAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTgg                                                                                                                         >  1:346965/1‑140 (MQ=255)        ccAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTgg                                                                                                                         <  1:473327/140‑1 (MQ=255)             aCATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGaa                                                                                                                    >  1:1125673/1‑140 (MQ=255)             aCATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTCCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGaa                                                                                                                    >  1:418189/1‑140 (MQ=255)                   tGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTa                                                                                                              <  2:878318/140‑1 (MQ=255)                   tGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTa                                                                                                              <  1:595979/140‑1 (MQ=255)                     ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACg                                                                                                            >  1:513938/1‑140 (MQ=255)                     ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACg                                                                                                            >  1:41327/1‑140 (MQ=255)                     ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACg                                                                                                            >  2:1352384/1‑140 (MQ=255)                     ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACg                                                                                                            >  2:1547134/1‑140 (MQ=255)                     ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACg                                                                                                            <  1:25047/140‑1 (MQ=255)                     ggCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACg                                                                                                            >  2:25047/1‑140 (MQ=255)                      gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACgg                                                                                                           >  2:836888/1‑140 (MQ=255)                      gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACgg                                                                                                           >  1:576995/1‑140 (MQ=255)                      gcgcgcGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACgg                                                                                                           >  1:71495/1‑140 (MQ=255)                       cgcgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACggg                                                                                                          <  1:747992/140‑1 (MQ=255)                        gcgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGc                                                                                                         <  1:1078397/140‑1 (MQ=255)                        gcgcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGc                                                                                                         >  1:1016636/1‑140 (MQ=255)                          gcgGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTg                                                                                                       >  2:1512901/1‑140 (MQ=255)                             gTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGtt                                                                                                    >  1:209716/1‑140 (MQ=255)                                  ttACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTttctt                                                                                               <  1:1512901/140‑1 (MQ=255)                                  ttACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTttctt                                                                                               <  2:576995/140‑1 (MQ=255)                                    aCCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTttcttct                                                                                             <  2:520746/140‑2 (MQ=255)                                      cacTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGGATACGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                                           <  1:933326/138‑1 (MQ=255)                                      cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                                           <  1:971899/140‑1 (MQ=255)                                      cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                                           <  1:1485397/140‑1 (MQ=255)                                      cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                                           <  1:1190260/140‑1 (MQ=255)                                      cGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGa                                                                                           <  1:628148/140‑1 (MQ=255)                                          gTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCa                                                                                       >  2:1490829/1‑140 (MQ=255)                                                aTCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGa                                                                                 >  1:389196/1‑140 (MQ=255)                                                               ggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAg                                                                  >  2:1121086/1‑140 (MQ=255)                                                               ggCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAg                                                                  >  2:1441117/1‑140 (MQ=255)                                                                   ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGCCTGCGCCGCCTTCAGtttt                                                              >  1:949429/1‑140 (MQ=255)                                                                   ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcg                                                                            >  2:1024491/1‑126 (MQ=255)                                                                   ttGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTgcg                                                                            <  1:1024491/126‑1 (MQ=255)                                                                                tGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTg                                                 >  2:380906/1‑140 (MQ=255)                                                                                tGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTg                                                 >  2:983827/1‑140 (MQ=255)                                                                                tGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTg                                                 >  1:428743/1‑140 (MQ=255)                                                                                  cTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                                               <  1:1121086/140‑1 (MQ=255)                                                                                  cTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                                               <  1:1441117/140‑1 (MQ=255)                                                                                  cTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                                               <  2:71495/140‑1 (MQ=255)                                                                                  cTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                                               <  2:428743/140‑1 (MQ=255)                                                                                  cTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                                               <  1:380906/140‑1 (MQ=255)                                                                                    cATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCAt                                             <  1:1490829/140‑1 (MQ=255)                                                                                     aTAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                                            >  1:780684/1‑140 (MQ=255)                                                                                     aTAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                                            <  2:389196/140‑1 (MQ=255)                                                                                     aTAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                                            <  2:209716/140‑1 (MQ=255)                                                                                       aaaGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTg                                          <  1:148779/140‑1 (MQ=255)                                                                                            tGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                                            >  1:412752/1‑133 (MQ=255)                                                                                            tGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATc                                            <  2:412752/133‑1 (MQ=255)                                                                                            tGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTa                                     >  1:340920/1‑140 (MQ=255)                                                                                              aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGt                                   >  2:1434152/1‑140 (MQ=255)                                                                                              aagaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGGTTTACCAAATTTGGTGCCATCTGCTTTAGt                                   >  1:815140/1‑140 (MQ=255)                                                                                               agaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                                               <  2:378251/127‑1 (MQ=255)                                                                                               agaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGcc                                               >  1:378251/1‑127 (MQ=255)                                                                                               agaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTg                                  >  1:474262/1‑140 (MQ=255)                                                                                               agaaCTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTg                                  >  1:687195/1‑140 (MQ=255)                                                                                                   cTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCa                              >  1:748605/1‑140 (MQ=255)                                                                                                     tCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGc                            >  1:154053/1‑140 (MQ=255)                                                                                                      cAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCg                           >  2:479372/1‑140 (MQ=255)                                                                                                        ggAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGa                         <  2:41327/140‑1 (MQ=255)                                                                                                        ggAAGCGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGa                         <  1:836888/140‑1 (MQ=255)                                                                                                             cGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGt                    <  1:468123/140‑1 (MQ=255)                                                                                                             cGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGt                    <  2:748605/140‑1 (MQ=255)                                                                                                             cGGTAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGt                    <  2:637337/140‑1 (MQ=255)                                                                                                                tAAACGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAg                 >  1:409204/1‑140 (MQ=255)                                                                                                                 aaaCGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAgg                <  2:780684/140‑1 (MQ=255)                                                                                                                 aaaCGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAgg                <  2:154053/140‑1 (MQ=255)                                                                                                                    cGTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCa             <  2:687195/140‑1 (MQ=255)                                                                                                                     gTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCaa            <  1:1522738/140‑1 (MQ=255)                                                                                                                     gTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCaa            <  1:657434/140‑1 (MQ=255)                                                                                                                     gTCGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCaa            <  2:949429/140‑1 (MQ=255)                                                                                                                       cGGCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCAAac          >  1:1514653/1‑140 (MQ=255)                                                                                                                        ggCATCCGCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCAAaca         >  2:189551/1‑140 (MQ=255)                                                                                                                               gCAGTGTTGAACCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCAAACACCTGAtt  >  1:367038/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                  TGCTCCGCCAGTACATACTGGGCGCGCGGTGCTTTACCGCTGTTTTTATCTTCTTCTTCCAGGGCGTTGATCTCTTCAATGCTCATAAAGGTGAAGAACTTCAGGAAGCGGTAAACGTCGGCATCCGCAGTGTTGATCCAGAACTGGTAGAATTTGTACGGGCTGGTTTTCTTCGGATCCAACCAGACTGCGCCGCCTTCAGTTTTACCAAATTTGGTGCCATCTGCTTTAGTGATCAGCGGAACGGTCAGGCCAAACACCTGATT  >  NC_000913/1716306‑1716571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑