Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,641	G→A	D42N (GAT→AAT)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,641	0	G	A	100.0%	279.4 / NA	77	D42N (GAT→AAT)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (44/33);  total (44/33)

CAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGA  >  NC_000913/422505‑422777                                                                                                                                         |                                                                                                                                         caGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATa                                                                                                                                       >  2:69044/1‑140 (MQ=255)        aTTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACgaaga                                                                                                                                <  1:69044/140‑1 (MQ=255)        aTTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTACGTACAGAACACAATAACgaaga                                                                                                                                <  2:639860/140‑1 (MQ=255)            tgatgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATg                                                                                                                            >  1:112665/1‑140 (MQ=255)                 ttAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGt                                                                                                                       >  2:931878/1‑140 (MQ=255)                 ttAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGt                                                                                                                       >  2:863737/1‑140 (MQ=255)                 ttAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGt                                                                                                                       >  1:87132/1‑140 (MQ=255)                   aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTAc                                                                                                                     <  2:87132/140‑1 (MQ=255)                   aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTAc                                                                                                                     >  1:855492/1‑140 (MQ=255)                    aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTAcc                                                                                                                    <  2:631563/140‑1 (MQ=255)                    aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTAcc                                                                                                                    <  1:896026/140‑1 (MQ=255)                    aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTAcc                                                                                                                    <  2:112665/140‑1 (MQ=255)                         aTGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACgaaga                                                                                                                                >  2:782506/1‑123 (MQ=255)                         aTGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACgaaga                                                                                                                                <  1:782506/123‑1 (MQ=255)                         aTGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGc                                                                                                               >  1:529007/1‑140 (MQ=255)                          tGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTAcc                                                                                                                    >  2:230613/1‑134 (MQ=255)                          tGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTAcc                                                                                                                    <  1:230613/134‑1 (MQ=255)                           ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCAt                                                                                                             >  2:69125/1‑140 (MQ=255)                              aCCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAag                                                                                                          >  1:266523/1‑140 (MQ=255)                                  gCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAagcagc                                                                                                      >  1:596255/1‑140 (MQ=255)                                  gCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAagcagc                                                                                                      >  2:767656/1‑140 (MQ=255)                                   ccGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAgcg                                                                                                     <  2:230524/140‑1 (MQ=255)                                       tGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGt                                                                                                 <  2:266523/140‑1 (MQ=255)                                            ccATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACGCAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGcc                                                                                            <  1:118305/140‑1 (MQ=255)                                            ccATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGcc                                                                                            <  2:855492/140‑1 (MQ=255)                                                  gTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGcc                                                                                      >  2:214854/1‑140 (MQ=255)                                                   ttAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCt                                                                                     >  2:885357/1‑140 (MQ=255)                                                      aaCAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGc                                                                                  >  1:512037/1‑140 (MQ=255)                                                       aCAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCg                                                                                 <  1:213672/140‑1 (MQ=255)                                                         aaagcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTc                                                                               <  2:512037/140‑1 (MQ=255)                                                         aaagcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTc                                                                               <  1:214854/140‑1 (MQ=255)                                                           agcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAg                                                                                        <  2:220691/129‑1 (MQ=255)                                                           agcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAg                                                                                        >  1:220691/1‑129 (MQ=255)                                                           agcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCAc                                                                             >  1:423982/1‑140 (MQ=255)                                                                       cTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTgc                                                                 >  2:696199/1‑140 (MQ=255)                                                                           tCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTgcggcg                                                             <  1:96511/140‑1 (MQ=255)                                                                            cATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGa                                                            >  1:422517/1‑140 (MQ=255)                                                                            cATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGa                                                            <  1:696199/140‑1 (MQ=255)                                                                            cATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGa                                                            <  1:69125/140‑1 (MQ=255)                                                                              ttACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGAtt                                                          <  1:767656/140‑1 (MQ=255)                                                                               tACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTg                                                         >  1:743568/1‑140 (MQ=255)                                                                                 cacTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAt                                                       <  1:931878/140‑1 (MQ=255)                                                                                  acTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAGTGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtc                                                      >  2:870588/1‑140 (MQ=255)                                                                                    tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTgc                                                                 >  1:92164/1‑127 (MQ=255)                                                                                    tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTgc                                                                 <  2:92164/127‑1 (MQ=255)                                                                                    tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctc                                                    >  1:915020/1‑140 (MQ=255)                                                                                    tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctc                                                    >  2:812521/1‑140 (MQ=255)                                                                                      tgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctctc                                                  >  1:16748/1‑140 (MQ=255)                                                                                       ggCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTcc                                                 >  2:758886/1‑140 (MQ=255)                                                                                       ggCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTcc                                                 >  2:790698/1‑140 (MQ=255)                                                                                       ggCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTcc                                                 >  2:613048/1‑140 (MQ=255)                                                                                        gCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCa                                                <  2:348163/140‑1 (MQ=255)                                                                                         cTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAg                                               >  1:52456/1‑140 (MQ=255)                                                                                         cTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAg                                               <  2:743568/140‑1 (MQ=255)                                                                                            aCGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAt                                                       <  1:79193/129‑1 (MQ=255)                                                                                            aCGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAt                                                       >  2:79193/1‑129 (MQ=255)                                                                                              gggCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGa                                                            >  2:274607/1‑122 (MQ=255)                                                                                              gggCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGa                                                            <  1:274607/122‑1 (MQ=255)                                                                                                gCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACaa                                        >  1:361486/1‑140 (MQ=255)                                                                                                gCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACaa                                        >  2:775865/1‑140 (MQ=255)                                                                                                      aCCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCcgg                                  <  1:613048/140‑1 (MQ=255)                                                                                                      aCCCACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCcgg                                  <  1:812521/140‑1 (MQ=255)                                                                                                       cccACCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAGTGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCcggc                                 <  1:870588/140‑1 (MQ=255)                                                                                                          aCCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGAc                                          >  1:152762/1‑128 (MQ=255)                                                                                                          aCCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGAc                                          <  2:152762/128‑1 (MQ=255)                                                                                                          aCCGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCggcggc                              >  2:722456/1‑140 (MQ=255)                                                                                                            cGCAGCGCATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGt                            <  2:16748/140‑1 (MQ=255)                                                                                                                gcgcATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACa                        >  1:836277/1‑140 (MQ=255)                                                                                                                 cgcATTATGCTGCGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAg                       <  1:722456/140‑1 (MQ=255)                                                                                                                             cGTACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAActgct           >  2:682433/1‑140 (MQ=255)                                                                                                                               tACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCtgt         >  1:902827/1‑140 (MQ=255)                                                                                                                               tACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCtgt         >  1:741468/1‑140 (MQ=255)                                                                                                                               tACAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCtgt         >  2:501558/1‑140 (MQ=255)                                                                                                                                aCAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGtt                     >  2:73146/1‑127 (MQ=255)                                                                                                                                aCAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGtt                     <  1:73146/127‑1 (MQ=255)                                                                                                                                 cAGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTgg       >  2:217604/1‑140 (MQ=255)                                                                                                                                  aGAACACAATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGc      >  1:974256/1‑140 (MQ=255)                                                                                                                                      cacaATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGa  >  1:815344/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                         CAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCCAGCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGA  >  NC_000913/422505‑422777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑