Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	683,868	A→C	I182S (ATC→AGC)	rihA ←	pyrimidine‑specific ribonucleoside hydrolase RihA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	683,868	0	A	C	100.0%	176.7 / NA	46	I182S (ATC→AGC)	rihA	pyrimidine‑specific ribonucleoside hydrolase RihA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (22/24);  total (22/24)

GATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCAATTTTGCTATG  >  NC_000913/683734‑683992                                                                                                                                       |                                                                                                                             gATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCt                                                                                                                         <  1:521988/140‑1 (MQ=255)      tCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTg                                                                                                                    <  2:572662/140‑1 (MQ=255)      tCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTg                                                                                                                    >  1:711092/1‑140 (MQ=255)      tCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTg                                                                                                                    >  1:409720/1‑140 (MQ=255)          ggAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAg                                                                                                                >  1:380909/1‑140 (MQ=255)                 aTCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAAttt                                                                                                         <  2:722469/140‑1 (MQ=255)                    cagcagTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGCCTGGAAGACAATTTCTg                                                                                                      <  2:711092/140‑1 (MQ=255)                     agcagTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGc                                                                                                     <  1:309117/140‑1 (MQ=255)                      gcagTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGcc                                                                                                    >  1:455844/1‑140 (MQ=255)                          ttCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCtt                                                                                                >  2:855777/1‑140 (MQ=255)                            cGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTcc                                                                                              >  2:325720/1‑140 (MQ=255)                               caacaaTGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCggg                                                                                           <  1:408062/140‑1 (MQ=255)                                 acaaTGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTc                                                                                         >  1:657614/1‑140 (MQ=255)                                    aTGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTg                                                                                                      >  2:738976/1‑124 (MQ=255)                                    aTGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTg                                                                                                      <  1:738976/124‑1 (MQ=255)                                    aTGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAAc                                                                                      >  1:885021/1‑140 (MQ=255)                                    aTGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAAc                                                                                      >  1:593945/1‑140 (MQ=255)                                     tGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGcc                                                                                                    >  2:53464/1‑125 (MQ=255)                                     tGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGcc                                                                                                    <  1:53464/125‑1 (MQ=255)                                           aaaCAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAata                                                                               <  1:575537/140‑1 (MQ=255)                                              cAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTa                                                                            <  1:846136/140‑1 (MQ=255)                                                 ggTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAAt                                                                         >  1:612911/1‑140 (MQ=255)                                                 ggTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAAt                                                                         >  2:550713/1‑140 (MQ=255)                                                 ggTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAAt                                                                         >  2:398998/1‑140 (MQ=255)                                                    tACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCa                                                                      <  2:804484/140‑1 (MQ=255)                                                      ccAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGc                                                                    <  2:593945/140‑1 (MQ=255)                                                           cgcgcgGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAg                                                               >  1:621712/1‑140 (MQ=255)                                                             cgcgGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGc                                                             >  1:312998/1‑140 (MQ=255)                                                             cgcgGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGc                                                             >  2:291457/1‑140 (MQ=255)                                                              gcgGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCg                                                            <  2:885021/140‑1 (MQ=255)                                                              gcgGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCg                                                            <  1:398998/140‑1 (MQ=255)                                                              gcgGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCg                                                            <  1:325720/140‑1 (MQ=255)                                                              gcgGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCg                                                            <  1:238442/140‑1 (MQ=255)                                                                        tCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTAcc                                                  <  2:409720/140‑1 (MQ=255)                                                                        tCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTAcc                                                  <  2:190817/140‑1 (MQ=255)                                                                        tCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTAcc                                                  <  1:306148/140‑1 (MQ=255)                                                                                     cGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAgcg                                     <  2:657614/140‑1 (MQ=255)                                                                                        ggATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCa                                  >  2:467721/1‑140 (MQ=255)                                                                                                  tttATGAGTAACATCCAGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCa                        <  2:612911/140‑1 (MQ=255)                                                                                                                  aGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCaa            >  2:465051/1‑136 (MQ=255)                                                                                                                  aGACCGGCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCaa            <  1:465051/136‑1 (MQ=255)                                                                                                                       ggCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCAATTTTGCTAt   <  1:677398/140‑1 (MQ=255)                                                                                                                        gCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCAATTTTGCTATg  <  1:859535/140‑1 (MQ=255)                                                                                                                        gCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCAATTTTGCTATg  >  1:305537/1‑140 (MQ=255)                                                                                                                        gCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCAATTTTGCTATg  >  2:84509/1‑140 (MQ=255)                                                                                                                        gCCATCACCACCGGGCTCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCAATTTTGCTATg  >  1:150191/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                             GATATTCGAGGAAGAAATCCAGCAGTTCGGCAACAATGGTTGAAACAGGGTTACCAATCGCGCGGAAACGCTCGGTGTCTTCAACGTGGATTTGTGCTTTATGAGTAACATCCAGACCGGCCATCACCACCGGGATCCCTGACTGGAAGACAATTTCTGCCGCTTCCGGGTCAACGTAAATATTAAATTCAGCCGCAGGCGTCCAGTTACCCAGCCCCATAGCGCCACCCATAATCACGATACGGGCAATTTTGCTATG  >  NC_000913/683734‑683992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑