| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,206,435 | 0 | C | T | 32.3% | 40.5 / 18.3 | 31 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base C (0/21); new base T (10/0); total (10/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.25e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAA > NC_000913/3206298‑3206569 | aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCa < 1:1457138/140‑1 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGAcaaaaacaaaaa > 1:124998/1‑140 (MQ=255) gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTgg < 1:1113507/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACTTATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg < 1:357606/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg < 2:999090/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg < 2:539604/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg < 2:124998/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg < 1:81076/140‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAg < 1:1304654/140‑1 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACTTATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTAAATTTTTTCTGAAAAAAAAAAAAAAT‑GAGTTgtccaga > 2:357606/1‑134 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAAAAGAGTTGTCatga > 2:1304654/1‑140 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg < 1:1112492/140‑1 (MQ=255) ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa < 2:1455889/140‑1 (MQ=255) tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAACTGGAGTTGCCATGATGAGCGAAAga > 1:1263438/1‑139 (MQ=255) gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAGGAAAAAGaaa > 1:562702/1‑140 (MQ=255) ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc < 2:1333804/140‑1 (MQ=255) ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAATAAAAAAAATGGAGTTGCCATGGTGAGCGGAAGGAAAAAAAAACAAGCAGGGAATaaat > 2:990329/1‑138 (MQ=255) tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga < 1:1509029/140‑1 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAt < 1:990329/140‑1 (MQ=255) atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAAATTTTTCAGACAAAAAAAAAAAATAGAGGTGGCATGATGAGGGAAAGTAAAAAACAAAAAGCAGGGAATAAGATGACAaagaa > 2:839525/1‑139 (MQ=255) aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc < 1:1311211/140‑1 (MQ=255) gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTTACACAAAAACAAAAACTGGAGTTGCCATGATGGGCGGAAAAAAAAAACAAAAAGCAGTGAATAAAATTAAAAAGATTTTCGcc > 2:1412477/1‑139 (MQ=255) aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa < 1:817451/140‑1 (MQ=255) gatatAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc < 1:1279779/139‑1 (MQ=255) caTTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCCTGATGGGGGGAAATAATAAACAAAAAGCAGGGAATAAATTGACAGAGATTGTCGCTAAATTTTCCGCCCTGATTTcc > 2:1279779/1‑139 (MQ=255) ttAGTGGTAGTTTGTGGGGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg < 1:1412477/140‑1 (MQ=255) ggTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa < 1:576475/139‑1 (MQ=255) gTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt < 2:1263438/140‑1 (MQ=255) tttGTGGCGG‑TGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGAAAAAAAAAAAAAGCAGGGAAAAAATTGACAGAGAATGTTGCTAACTTTACCCCCCTGATTTTTACCCCAATGCCtgat > 2:1441637/1‑140 (MQ=255) tgtgGCGG‑TGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAATAATAAAAAAAAAGCAGGGAAAAAATTGACAGAGATTGTCGCTAACTTTACCCCCCCGATTTTTTCCCCAAAGCCtgatga > 2:440000/1‑140 (MQ=255) g‑TGAATTTTTTCAGACAAAAACAAAAAAAGGAGGTGGCATGATGAGCGGAAGGAAAAAGCAACAAGCAGGGAAAAAATTGACAGAGATTGTCGCTAAATTTACCGCCATGATTTTTACCCCAATGCCTGATGACgggggg > 2:1478101/1‑138 (MQ=255) g‑TGAAATTTTTCACACAAATACAAAAAAAGGGGGTGGCATCAAGAGGGAAAGGAAAAAACAACAAGCAGTGAATAAGTTGACAGAGAATGTCGCTAAATTTAACCCCATGATTTTTACCCCAATACCCGATGAAggggtg > 2:805066/1‑140 (MQ=255) gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 1:1478101/140‑1 (MQ=255) aaaTTTTTCCAGAAAAATACAAAAACTGGATTTCCCATGATGAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa < 2:562702/139‑1 (MQ=255) tttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCCTGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa < 1:440000/140‑1 (MQ=255) | AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGG‑TGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAA > NC_000913/3206298‑3206569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |