Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	714,605	G→C	V350L (GTT→CTT)	pgm →	phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	714,605	0	G	C	100.0%	244.7 / NA	63	V350L (GTT→CTT)	pgm	phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (31/32);  total (31/32)

GACCGTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGG  >  NC_000913/714479‑714738                                                                                                                               |                                                                                                                                      gACCGTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCgat                                                                                                                          <  2:69978/140‑1 (MQ=255)     gTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATc                                                                                                                      >  2:684464/1‑140 (MQ=255)     gTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATc                                                                                                                      >  2:608072/1‑140 (MQ=255)     gTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATc                                                                                                                      >  2:406046/1‑140 (MQ=255)     gTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATc                                                                                                                      >  2:842652/1‑140 (MQ=255)     gTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATc                                                                                                                      >  2:827075/1‑140 (MQ=255)      tCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCg                                                                                                                     >  1:52817/1‑140 (MQ=255)      tCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCg                                                                                                                     >  2:73644/1‑140 (MQ=255)      tCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCg                                                                                                                     >  2:762271/1‑140 (MQ=255)      tCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCg                                                                                                                     >  1:877137/1‑140 (MQ=255)      tCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCg                                                                                                                     >  2:835987/1‑140 (MQ=255)      tCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCg                                                                                                                     >  2:183125/1‑140 (MQ=255)       cACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGa                                                                                                                    <  2:877137/140‑1 (MQ=255)         cGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGAcc                                                                                                                  <  1:406046/140‑1 (MQ=255)         cGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGAcc                                                                                                                  <  1:827075/140‑1 (MQ=255)            tATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCgtg                                                                                                               <  1:1184342/140‑1 (MQ=255)             aTCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCgtgt                                                                                                              <  2:1179396/140‑1 (MQ=255)              tCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGtgtg                                                                                                             <  1:370785/140‑1 (MQ=255)                 tCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCATCATCGTCCGCAGTTGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTc                                                                                                          >  2:365410/1‑140 (MQ=255)                 tCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTc                                                                                                          >  1:511348/1‑140 (MQ=255)                 tCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTc                                                                                                          >  1:1074201/1‑140 (MQ=255)                  cACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCa                                                                                                         <  2:876851/140‑1 (MQ=255)                   aCTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCaa                                                                                                        >  1:411433/1‑140 (MQ=255)                    cTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAAc                                                                                                       >  2:1303320/1‑140 (MQ=255)                        ggCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCgtg                                                                                                               >  1:520/1‑128 (MQ=255)                        ggCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCgtg                                                                                                               <  2:520/128‑1 (MQ=255)                               ttGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCg                                                                                            <  2:1048688/140‑1 (MQ=255)                                 gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTa                                                                                          <  1:973166/140‑1 (MQ=255)                                 gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTa                                                                                          <  1:183125/140‑1 (MQ=255)                                 gatgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTa                                                                                          <  2:989886/140‑1 (MQ=255)                                  atgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaa                                                                                         <  2:1074201/140‑1 (MQ=255)                                   tgaATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaaa                                                                                        <  2:511348/140‑1 (MQ=255)                                     aaTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACt                                                                                      <  1:762271/140‑1 (MQ=255)                                     aaTCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACt                                                                                      <  1:835987/140‑1 (MQ=255)                                       tCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTgg                                                                                    >  2:708948/1‑140 (MQ=255)                                           aaCCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGa                                                                                >  2:1524378/1‑140 (MQ=255)                                            aCCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAAc                                                                                       <  2:989126/132‑1 (MQ=255)                                            aCCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAAc                                                                                       >  1:989126/1‑132 (MQ=255)                                             ccACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAg                                                                              <  2:411433/140‑1 (MQ=255)                                                  aCCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaa                                                                                         <  2:950051/124‑1 (MQ=255)                                                  aCCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTaa                                                                                         >  1:950051/1‑124 (MQ=255)                                                   ccTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCgg                                                                        <  1:1524378/140‑1 (MQ=255)                                                   ccTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGAGGTGGCCGGCGGTAAACCGGTACTTGCATCTGCGATGATCGAGCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCgg                                                                        <  2:1502010/140‑1 (MQ=255)                                                    cTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGt                                                                       <  1:608072/140‑1 (MQ=255)                                                     tGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTa                                                                      >  1:1161215/1‑140 (MQ=255)                                                             gCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCaa                                                              >  1:849217/1‑140 (MQ=255)                                                             gCAATCAATTACCTGTTCCAGCATCGGCCGCAGTGGGGCAAAAATGCTGCCGTCGGTAAAACGCTGCTTTCATCTTCGATGATCGACCGGGTGGGCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGCTAGGTTTCaa                                                              >  2:1524782/1‑140 (MQ=255)                                                              caatcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCaaa                                                             <  2:576715/140‑1 (MQ=255)                                                                 tcaatTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATgg                                                          >  1:1000805/1‑140 (MQ=255)                                                                           gTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATg                                                <  1:1303320/140‑1 (MQ=255)                                                                           gTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATg                                                >  1:1001332/1‑140 (MQ=255)                                                                           gTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATg                                                >  2:1167469/1‑140 (MQ=255)                                                                            ttCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATgg                                               <  2:1161215/140‑1 (MQ=255)                                                                              ccAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCaaa                                                             <  1:1314267/124‑1 (MQ=255)                                                                              ccAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCaaa                                                             >  2:1314267/1‑124 (MQ=255)                                                                              ccAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTc                                             <  1:684464/140‑1 (MQ=255)                                                                              ccAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTc                                             <  1:842652/140‑1 (MQ=255)                                                                                      gTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAAC‑GGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATg                                                <  1:197382/128‑1 (MQ=255)                                                                                      gTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAAC‑GGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATg                                                >  2:197382/1‑128 (MQ=255)                                                                                       tCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACg                                    >  2:416811/1‑140 (MQ=255)                                                                                       accGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTc                                             <  1:1482524/130‑1 (MQ=255)                                                                                       accGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTc                                             >  2:1482524/2‑131 (MQ=255)                                                                                                 ggCAAAGATGTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCgg                          >  1:65256/1‑140 (MQ=255)                                                                                                          gTTGCCGTCGGTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTggcgg                 >  1:756508/1‑140 (MQ=255)                                                                                                                   ggTAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAagagag        >  1:436250/1‑140 (MQ=255)                                                                                                                     tAAAACGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTg      <  1:708948/140‑1 (MQ=255)                                                                                                                         aCGCTGCTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAgg  <  2:436250/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                      GACCGTCACGGTATCGTCACTCCGGCAGGTTTGATGAATCCGAACCACTACCTGGCGGTGGCAATCAATTACCTGTTCCAGCATCGTCCGCAGTGGGGCAAAGATGTTGCCGTCGGTAAAACGCTGGTTTCATCTGCGATGATCGACCGTGTGGTCAACGACTTGGGCCGTAAACTGGTAGAAGTCCCGGTAGGTTTCAAATGGTTTGTCGATGGTCTGTTCGACGGCAGCTTCGGCTTTGGCGGCGAAGAGAGTGCAGG  >  NC_000913/714479‑714738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑