Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT32.0% 254.4 / 66.1 103N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (70/0);  new base T (0/33);  total (70/33)
Fisher's exact test for biased strand distribution p-value = 1.05e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885
                                                                                                                                         |                                                                                                                                            
ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGtttt                                                                                                                                          >  2:1275864/1‑140 (MQ=255)
ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGtttt                                                                                                                                          >  1:2969141/1‑140 (MQ=255)
 tGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGttttt                                                                                                                                         >  1:1345793/1‑140 (MQ=255)
 tGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGttttt                                                                                                                                         >  1:2343260/1‑140 (MQ=255)
   ttaTTTATTCAACCCTTCAAGCGATTCAGATACCCCCATCTTAATCGGTTAAACAGCGAAGGTCACCCCTTTTC‑CGCCGTTGC‑CCGCAACAACATACCGCAGTGCCCCTTCTGTCTGGGTGAAATAA‑TTTTTTTTTT‑cc‑c                                                                                                                                    <  2:320002/138‑1 (MQ=255)
     ttttATTAAAACCTCAAAGCGATTCAGAGAGCGCCAGCTTAATCGGTCAAACAGCGAAGGCCCCCCCCTTTC‑CGCCGTTGC‑CCGCAAAAACATAACGCAGTGCCCCTTCTTTCTCGGTGTAATAACTTTTTTTTTC‑cc‑cc                                                                                                                                   <  2:1625654/139‑1 (MQ=255)
      tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑ccg                                                                                                                                  >  1:2925801/1‑140 (MQ=255)
               aCCTTCAAGCGATTCAGATAGCACCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTtagta                                                                                                                         >  1:1282958/1‑140 (MQ=255)
                   tCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGa                                                                                                                     >  1:1032318/1‑140 (MQ=255)
                    cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGAc                                                                                                                    >  2:2802152/1‑140 (MQ=255)
                    cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGAc                                                                                                                    >  2:1231231/1‑140 (MQ=255)
                    cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGAc                                                                                                                    >  1:3341814/1‑140 (MQ=255)
                    cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGAGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGAc                                                                                                                    >  2:1611289/1‑140 (MQ=255)
                     aaGCGATTCAGATCGCCCCATCTTAACCGTTCCAAAAGCGAAGGCCACCCCCTTTC‑CGCCGTTGT‑CCGCGACAACATAACGCAGTCCCCTTTCTGTCTGGGTGTAATAACTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACt                                                                                                                   <  2:1400926/140‑1 (MQ=255)
                           ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGtt                                                                                                             >  2:775920/1‑140 (MQ=255)
                           ttCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGtt                                                                                                             >  1:2234201/1‑140 (MQ=255)
                               gATAGCCCCATCTTACTCGTTTCAACAGCGAAGGTCCCCCCTTTTT‑CGCCTTTGT‑CCGCGACAACATAACGCAGTCCCCTTTCTGTCTGGGTTTAATAACGTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGtttttt                                                                                                         <  2:607037/140‑1 (MQ=255)
                                aTAGCCCCAGTTTAATCGGTTCAACAGCGAGGGTCCCCCCTTTTC‑CGCCGTTGC‑CCGCGACAACATAACGCGGTCCCCCTTCTGTCTCGGTGTAATACCGTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTc                                                                                                        <  2:432292/140‑1 (MQ=255)
                                     gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  1:1869401/1‑140 (MQ=255)
                                      ccAGTTTAATCGTTTCAACGGCGAGGGTCACCCCTTTTC‑CGCCGTTGC‑CCGCGACAACATACCGCAGTGCCCCTTCTGTCTGGGTTTAATATTTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCtt                                                                                                  <  2:2925801/140‑1 (MQ=255)
                                       cAGCTTAATCGGTCCAACAGCGAGGGTCACCCCTTTTT‑CGCCTTTGT‑CCGCGACAACATACCGCAGTGCCCCTTCTGTCTGGGTGTAATAACTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCttt                                                                                                 <  2:1705215/140‑1 (MQ=255)
                                         gCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgt                                                                                               >  1:2359175/1‑140 (MQ=255)
                                          cTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGGGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTtgtg                                                                                              >  1:307580/1‑140 (MQ=255)
                                          cTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGC‑CCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTgtgt                                                                                             >  2:2168774/1‑140 (MQ=255)
                                            tAATCGTTTCAACAGCAAAGGCCCCCCCTTTTT‑CGCCTTTGC‑CCGCCAAAACAAAACGCAGTCCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtt                                                                                            <  2:1896310/140‑1 (MQ=255)
                                            tAATCGGTCCAACAGCGAGGGCCCCCCCTTTTC‑CGCCGTTGT‑CCGCAACAACATACCGCAGTGCCCCTTCTGTCTCGGTTTAATAATTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtt                                                                                            <  2:94464/140‑1 (MQ=255)
                                              aTCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGtttt                                                                                          >  1:1962662/1‑140 (MQ=255)
                                                 gttcaaaCAGGGAGGGCCACCCCTTTTT‑CGCCGTTGC‑CCGCAACAACATACCGCAGTCCCCTTTCTGTCTGGGTGTAATACCTTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGt                                                                                       <  2:1749804/135‑1 (MQ=255)
                                                   ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:1230749/1‑140 (MQ=255)
                                                   ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:3166705/1‑140 (MQ=255)
                                                   ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:485362/1‑140 (MQ=255)
                                                   ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                     >  1:767949/1‑140 (MQ=255)
                                                    tCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    >  2:1835111/1‑140 (MQ=255)
                                                    tCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    >  2:2599520/1‑140 (MQ=255)
                                                    tCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                    >  1:1866377/1‑140 (MQ=255)
                                                       aCAGCGAAGGTCACCCCCTTTT‑CGCCGTTGC‑CCGCGAAAACATAACGCAGTCCCCCTTCTGTCTGGGTGAAATAACTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTaa                                                                                 <  2:2167378/140‑1 (MQ=255)
                                                        cAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGCTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAAt                                                                                >  2:2624045/1‑140 (MQ=255)
                                                           cGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                                                                             >  1:1549332/1‑140 (MQ=255)
                                                             aaGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           >  1:1787370/1‑140 (MQ=255)
                                                             aaGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           >  1:2542811/1‑140 (MQ=255)
                                                             aaGGTCACCCCTTTTT‑GGCCGTTGT‑CCGGGACAACAAAGGGCGTTGCCCTTTTTGGCTGGGTTAAATACCGTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:172395/140‑1 (MQ=255)
                                                             aaGGCCACCCCTTTTC‑CGCCTTCGC‑CGGCAACAACAAACCGAAGTGCCCCTGCGGTCTGGGTATAA‑AACGTTTTTTTTA‑CCGCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:1962662/140‑1 (MQ=255)
                                                              aaggcccccccTTTTCCGCCGTTGC‑CCGCGACAACATACCGCGGTGCCCCTTCTGTCGGGGTGTAATATTTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:2359175/133‑1 (MQ=255)
                                                               ggTCACCCCTTTTT‑CGCCGTTGC‑CCGCGAAAACAAACCGCGGTGCCCCTTCTGTCTGGGTTTAATACTTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa                                                                         <  2:3005643/140‑1 (MQ=255)
                                                                 cccccccctttt‑ccccttttC‑CCGCGACAACATAACGCGGTGCACTTCCTTTCTGTGTGTAAAATTTTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgt                                                                       <  2:307580/136‑1 (MQ=255)
                                                                   aGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAgtgt                                                                     >  1:1027702/1‑140 (MQ=255)
                                                                     cccctttt‑ccccttttCCCCGCAACAACATAACGCGTTCCCCTTTTTTTCTGGGT‑TAAAAACTTTTTTTTTTCCC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGtgtg                                                                    <  2:1869401/140‑1 (MQ=255)
                                                                          ttt‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                              >  1:1914957/1‑140 (MQ=255)
                                                                               gcggTTGT‑CCCCGACAACATAACGAGGACCTCTTTCTGTCCCGGTTTAATAAGTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCg                                                          <  1:1611289/137‑1 (MQ=255)
                                                                               gCCGTTGC‑CCGCAACAACATAACGCAGTGCCCTTTCTGTCTGGGTGTAATATCTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCg                                                          <  2:2542811/140‑1 (MQ=255)
                                                                                   gttccccGCAACAACATAACGCAGTCCCCCTTTTTTCTGGGTGTAATATTTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  2:3249112/135‑1 (MQ=255)
                                                                                     tgcccGCGACAACATACCGCAGTGCCCCTTCTGTCTGGGTGTAATAGTGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc                                                     <  2:2234201/137‑1 (MQ=255)
                                                                                        ccGCGACAACATACCGCAGTCCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATg                                                  <  1:1275864/140‑1 (MQ=255)
                                                                                        ccGCAACAACATACCGCAGTCCCCTTTCTGTCTGGGTGTAATATTTTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATg                                                  <  2:2773875/140‑1 (MQ=255)
                                                                                                 caTAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGa                                         >  2:2937130/1‑140 (MQ=255)
                                                                                                      cGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgcttt                                    >  2:2112640/1‑140 (MQ=255)
                                                                                                        cAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcg                                  >  2:2268143/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:3010696/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:2863000/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:1768443/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:3071475/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:325850/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:3128277/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:3302692/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:2755038/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:1278672/1‑140 (MQ=255)
                                                                                                           tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:1356809/1‑140 (MQ=255)
                                                                                                            gCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgcttt                              >  1:566537/1‑140 (MQ=255)
                                                                                                             cACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctttc                             >  2:2403279/1‑140 (MQ=255)
                                                                                                              cccTTCTGTCTGGGTGTAATACCTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGctttcgctttcg                            <  1:556925/139‑1 (MQ=255)
                                                                                                               ccTTCTTTCTGGGTTTAATAATTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCtttcgctttcgc                           <  2:667517/140‑1 (MQ=255)
                                                                                                                tttttGTCTCGGTAAAATATTTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                          <  2:1027702/136‑1 (MQ=255)
                                                                                                                cttcttTCTGGGTGTAATATCTTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                          <  2:197558/140‑1 (MQ=255)
                                                                                                                cTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                          >  1:2422191/1‑140 (MQ=255)
                                                                                                                     gTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                     >  1:197558/1‑140 (MQ=255)
                                                                                                                          ggTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                >  2:1038834/1‑140 (MQ=255)
                                                                                                                          ggTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                >  1:1344667/1‑140 (MQ=255)
                                                                                                                           ttgtAATATTTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc               <  2:1898151/139‑1 (MQ=255)
                                                                                                                           gtgtAATAACGTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc               <  1:2599520/140‑1 (MQ=255)
                                                                                                                            tgtAATATCTTTTTTTTTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              <  1:2802152/140‑1 (MQ=255)
                                                                                                                            tgtAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              >  2:2667804/1‑140 (MQ=255)
                                                                                                                            tgtAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              >  1:3366393/1‑140 (MQ=255)
                                                                                                                               aatatttttttttttC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGc           <  2:3337890/133‑1 (MQ=255)
                                                                                                                               aataaCTTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGc           <  1:903033/140‑1 (MQ=255)
                                                                                                                                atatctttttttttC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc          <  2:1401836/134‑1 (MQ=255)
                                                                                                                                ataaTGTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc          <  1:3364514/140‑1 (MQ=255)
                                                                                                                                    ttttttttttC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      <  2:1787370/138‑1 (MQ=255)
                                                                                                                                    tgtttttttTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      <  2:521006/139‑1 (MQ=255)
                                                                                                                                    cGTTTTTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      <  1:3128277/140‑1 (MQ=255)
                                                                                                                                     gtttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGT‑TTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:1737145/1‑140 (MQ=255)
                                                                                                                                      tttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                     >  2:2542357/1‑123 (MQ=255)
                                                                                                                                      tttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc               <  1:547977/129‑1 (MQ=255)
                                                                                                                                      tttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctc               >  2:547977/1‑129 (MQ=255)
                                                                                                                                      tttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:2499508/1‑140 (MQ=255)
                                                                                                                                      tttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:966484/1‑140 (MQ=255)
                                                                                                                                      tttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:1444048/1‑140 (MQ=255)
                                                                                                                                      tttgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:2255996/1‑140 (MQ=255)
                                                                                                                                        tgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                >  2:485026/1‑126 (MQ=255)
                                                                                                                                        tgtttTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  2:2995548/1‑140 (MQ=255)
                                                                                                                                        tgtttTC‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                <  1:485026/126‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                            
TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCCGTTGT‑CCGCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTT‑CC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑