Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,804,750	A→G	intergenic (+289/‑65)	nrdF → / → proV	ribonucleoside‑diphosphate reductase 2 subunit beta/glycine betaine ABC transporter ATP binding subunit ProV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,804,750	0	A	G	100.0%	285.6 / NA	73	intergenic (+289/‑65)	nrdF/proV	ribonucleoside‑diphosphate reductase 2 subunit beta/glycine betaine ABC transporter ATP binding subunit ProV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/53);  total (20/53)

GACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATAT  >  NC_000913/2804617‑2804886                                                                                                                                      |                                                                                                                                         gACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAg                                                                                                                                    >  2:60860/1‑140 (MQ=255)        aaTATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTAt                                                                                                                             <  1:1200374/140‑1 (MQ=255)        aaTATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTAt                                                                                                                             <  2:1153539/140‑1 (MQ=255)           atCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTc                                                                                                                          <  1:1336668/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  1:824939/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:1288488/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:374684/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:382084/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:1241950/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:51444/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  1:1358412/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:841223/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:1091595/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:95076/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  1:1177605/140‑1 (MQ=255)             cAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  2:1070247/140‑1 (MQ=255)               aCTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCCGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCAtt                                                                                                                      <  2:276119/140‑1 (MQ=255)                  ttCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTggg                                                                                                                   >  1:500776/1‑140 (MQ=255)                   tctcGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        >  2:429723/1‑134 (MQ=255)                   tctcGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCa                                                                                                                        <  1:429723/134‑1 (MQ=255)                   tctcGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGt                                                                                                                  <  2:916498/140‑1 (MQ=255)                   tctcGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGt                                                                                                                  <  2:500776/140‑1 (MQ=255)                   tctcGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGt                                                                                                                  <  2:332984/140‑1 (MQ=255)                     tcGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTaa                                                                                                                <  1:1175032/140‑1 (MQ=255)                       gATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATA‑TCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatat                                                                                                             >  1:947321/1‑140 (MQ=255)                         tttGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatata                                                                                                            <  2:987584/140‑1 (MQ=255)                          ttGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatatat                                                                                                           <  2:691584/140‑1 (MQ=255)                          ttGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatatat                                                                                                           <  1:1265409/140‑1 (MQ=255)                             ctctCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATACCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatatat                                                                                                           >  2:322693/1‑137 (MQ=255)                             ctctCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATACCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatatat                                                                                                           <  1:322693/137‑1 (MQ=255)                               ctcAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACa                                                                                                      <  2:1129054/140‑1 (MQ=255)                                  aGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAg                                                                                                   <  1:70021/140‑1 (MQ=255)                                    cccTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGAc                                                                                                 <  1:749102/140‑1 (MQ=255)                                       ttATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatata                                                                                                            >  2:214036/1‑126 (MQ=255)                                       ttATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAatata                                                                                                            <  1:214036/126‑1 (MQ=255)                                        tataTCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAAt                                                                                             <  2:1359794/140‑1 (MQ=255)                                            tCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTATGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAg                                                                                         >  1:787490/1‑140 (MQ=255)                                             cACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAgg                                                                                        <  1:818917/140‑1 (MQ=255)                                              aCGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAg                                                                                                   <  2:1206519/128‑1 (MQ=255)                                              aCGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAg                                                                                                   >  1:1206519/1‑128 (MQ=255)                                               cGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGaa                                                                                      >  1:672112/1‑140 (MQ=255)                                               cGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGaa                                                                                      <  1:291814/140‑1 (MQ=255)                                                 ggAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGaa                                                                                      <  2:479964/138‑1 (MQ=255)                                                 ggAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGaa                                                                                      >  1:479964/1‑138 (MQ=255)                                                 ggAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATc                                                                                    >  1:213254/1‑140 (MQ=255)                                                    aaTTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATaa                                                                                           <  2:781506/130‑1 (MQ=255)                                                    aaTTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATaa                                                                                           >  1:781506/1‑130 (MQ=255)                                                              aTTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATg                                                                       <  1:1336480/140‑1 (MQ=255)                                                                  gCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCaa                                                                   <  2:28900/140‑1 (MQ=255)                                                                         tCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCaattaaatt                                                            >  1:1256260/1‑140 (MQ=255)                                                                                  aTGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTaa                                                   >  2:764394/1‑140 (MQ=255)                                                                                   tGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTaaa                                                  <  2:997884/140‑1 (MQ=255)                                                                                       acaTTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATc                                              >  2:85242/1‑140 (MQ=255)                                                                                                    ggggTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATAtt                                 >  2:673300/1‑140 (MQ=255)                                                                                                     gggTTGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATAttt                                >  2:507591/1‑140 (MQ=255)                                                                                                         tGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCg                            <  2:213254/140‑1 (MQ=255)                                                                                                         tGCCTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCg                            <  2:793003/140‑1 (MQ=255)                                                                                                           ccTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAg                          >  2:508246/1‑140 (MQ=255)                                                                                                           ccTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAg                          <  1:107207/140‑1 (MQ=255)                                                                                                            cTCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGc                         <  2:1256260/140‑1 (MQ=255)                                                                                                             tCAGATTCTCAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCa                        <  2:49551/140‑1 (MQ=255)                                                                                                                      cAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcg               <  2:162475/140‑1 (MQ=255)                                                                                                                      cAGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcg               <  1:764394/140‑1 (MQ=255)                                                                                                                       aGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcg               >  2:135533/1‑139 (MQ=255)                                                                                                                       aGTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcg               <  1:135533/139‑1 (MQ=255)                                                                                                                        gTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgag             <  1:507591/140‑1 (MQ=255)                                                                                                                        gTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgag             <  1:508246/140‑1 (MQ=255)                                                                                                                        gTATGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgag             <  1:926335/140‑1 (MQ=255)                                                                                                                          aTGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgagcg           <  1:673300/140‑1 (MQ=255)                                                                                                                          aTGTTAGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgagcg           <  1:677336/140‑1 (MQ=255)                                                                                                                               aGGGTAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCaa      <  1:377456/140‑1 (MQ=255)                                                                                                                                   tAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACagcgag             <  1:1177541/129‑1 (MQ=255)                                                                                                                                   tAGGAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAatat  >  2:266283/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         GACAAAAAATATCAACTTTCTCGATTTGCTCTCAGCCCTTATATCACGGGAAATTCCGGCGATTTGCTCGCATCAATATTCATGCCACATTTGCCATCAGGGGTTGCCTCAGATTCTCAGTATGTTAGGGTAGAAAAAAGTGACTATTTCCATTGGGTAATATATCGACATAGACAAATAAAGGAATCTTTCTATTGCATGGCAATTAAATTAGAAATTAAAAATCTTTATAAAATATTTGGCGAGCATCCACAGCGAGCGTTCAAATAT  >  NC_000913/2804617‑2804886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑