Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,021,423	C→T	P400S (CCG→TCG)	ubiB →	ubiquinone biosynthesis protein UbiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,021,423	0	C	T	100.0%	240.5 / NA	69	P400S (CCG→TCG)	ubiB	ubiquinone biosynthesis protein UbiB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (39/30);  total (39/30)

ACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCC  >  NC_000913/4021289‑4021556                                                                                                                                       |                                                                                                                                      aCGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCaa                                                                                                                                  <  1:977013/140‑1 (MQ=255)     tGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGc                                                                                                                                    >  2:1314225/1‑134 (MQ=255)     tGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGc                                                                                                                                    <  1:1314225/134‑1 (MQ=255)      gAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGgt                                                                                                                             >  1:1273416/1‑140 (MQ=255)            ttCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACt                                                                                                                       >  1:805649/1‑140 (MQ=255)            ttCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACt                                                                                                                       >  1:650422/1‑140 (MQ=255)            ttCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACt                                                                                                                       <  2:1292881/140‑1 (MQ=255)              cGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTcc                                                                                                                     >  1:163863/1‑140 (MQ=255)              cGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTcc                                                                                                                     >  1:1206623/1‑140 (MQ=255)                 aTTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGa                                                                                                                  <  2:648603/140‑1 (MQ=255)                 aTTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGa                                                                                                                  <  2:718785/140‑1 (MQ=255)                  tttGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGaa                                                                                                                 <  1:1158374/140‑1 (MQ=255)                    tGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGaaaa                                                                                                               >  1:1348712/1‑140 (MQ=255)                      ccATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAAcc                                                                                                             >  1:1200925/1‑140 (MQ=255)                      ccATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAAcc                                                                                                             <  2:1125225/140‑1 (MQ=255)                           cGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGct                                                                                                        <  2:612463/140‑1 (MQ=255)                                   cTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCg                                                                                                <  2:1348712/140‑1 (MQ=255)                                   cTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCg                                                                                                <  2:650422/140‑1 (MQ=255)                                    tgtgAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGaaaa                                                                                                               <  2:3335/124‑1 (MQ=255)                                    tgtgAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGaaaa                                                                                                               >  1:3335/1‑124 (MQ=255)                                    tgtgAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGa                                                                                               >  1:210761/1‑140 (MQ=255)                                    tgtgAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGa                                                                                               >  1:222700/1‑140 (MQ=255)                                    tgtgAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGa                                                                                               >  1:303957/1‑140 (MQ=255)                                       gAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAgg                                                                                            <  2:694839/140‑1 (MQ=255)                                           cTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTa                                                                                        <  2:900362/140‑1 (MQ=255)                                           cTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTa                                                                                        <  2:717737/140‑1 (MQ=255)                                              tCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGa                                                                                     >  1:1157646/1‑140 (MQ=255)                                              tCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGa                                                                                     <  2:222700/140‑1 (MQ=255)                                              tCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGa                                                                                     >  2:1311877/1‑140 (MQ=255)                                                tttGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACg                                                                                   >  2:623121/1‑140 (MQ=255)                                                   gagaAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCa                                                                                <  2:889147/140‑1 (MQ=255)                                                        aCCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCttt                                                                           >  2:1377015/1‑140 (MQ=255)                                                        aCCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTAGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCttt                                                                           >  1:538194/1‑140 (MQ=255)                                                           gCTGGCAGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATc                                                                        <  2:1337095/140‑1 (MQ=255)                                                              ggCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGc                                                                     >  2:837930/1‑140 (MQ=255)                                                              ggCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGc                                                                     <  2:210761/140‑1 (MQ=255)                                                                  gAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACt                                                                 <  2:805649/140‑1 (MQ=255)                                                                                    gTACTGTTAAATCTGTTTAATACGGCGCGTAGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGc                                               <  2:538194/140‑1 (MQ=255)                                                                                     tACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCg                                              >  1:1209796/1‑140 (MQ=255)                                                                                          ttAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGcc                                         >  2:299866/1‑140 (MQ=255)                                                                                            aaaTCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGaaa                                                    <  1:693580/127‑1 (MQ=255)                                                                                            aaaTCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGaaa                                                    >  2:693580/1‑127 (MQ=255)                                                                                            aaaTCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGTGAAGCCtt                                       >  2:879639/1‑140 (MQ=255)                                                                                              aTCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTc                                     >  1:692933/1‑140 (MQ=255)                                                                                               tCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGc                                          <  1:110227/134‑1 (MQ=255)                                                                                               tCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGc                                          >  2:110227/1‑134 (MQ=255)                                                                                                 tGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTg                                  <  2:858725/140‑1 (MQ=255)                                                                                                  gTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTgg                                 >  1:702/1‑140 (MQ=255)                                                                                                  gTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTgg                                 >  2:1236180/1‑140 (MQ=255)                                                                                                    ttAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTTGAg                               >  2:718342/1‑140 (MQ=255)                                                                                                    ttAATACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAg                               >  2:213189/1‑140 (MQ=255)                                                                                                      aaTACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTc                             <  2:1206623/140‑1 (MQ=255)                                                                                                       aTACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCg                            <  1:1377015/140‑1 (MQ=255)                                                                                                        tACGGCGCGTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGt                           >  2:567749/1‑140 (MQ=255)                                                                                                             cgcgTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGAtt                      >  1:342470/1‑140 (MQ=255)                                                                                                             cgcgTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGAtt                      >  2:821615/1‑140 (MQ=255)                                                                                                             cgcgTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGAtt                      >  2:1034609/1‑140 (MQ=255)                                                                                                             cgcgTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGAtt                      >  2:1115296/1‑140 (MQ=255)                                                                                                             cgcgTCGCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGAtt                      >  1:327392/1‑140 (MQ=255)                                                                                                                   gCTTCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGAt                <  2:327392/140‑1 (MQ=255)                                                                                                                      tCAATATGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAg             <  2:303957/140‑1 (MQ=255)                                                                                                                           atGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCgg        <  1:1115296/140‑1 (MQ=255)                                                                                                                           atGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCgg        <  1:821615/140‑1 (MQ=255)                                                                                                                            tGGAAGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGt       >  1:1276536/1‑140 (MQ=255)                                                                                                                               aaGTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTAtt    >  1:1182634/1‑140 (MQ=255)                                                                                                                               aaGTGCAGTCGCAACTGGTGTTACTACAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTAtt    >  1:684525/1‑140 (MQ=255)                                                                                                                                 gTGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTcc  >  1:815468/1‑140 (MQ=255)                                                                                                                                  tGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCgg        >  1:218081/1‑133 (MQ=255)                                                                                                                                  tGCAGTCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCgg        <  2:218081/133‑1 (MQ=255)                                                                                                                                       |                                                                                                                                      ACGTTGAAGAGTTCGAATTTGCCATTCGTACGGTCTGTGAACCTATCTTTGAGAAACCGCTGGCCGAAATTTCGTTTGGACATGTACTGTTAAATCTGTTTAATACGGCGCGTCGCTTCAATATGGAAGTGCAGCCGCAACTGGTGTTACTCCAGAAAACCCTGCTCTACGTCGAAGGGGTAGGACGCCAGCTTTATCCGCAACTCGATTTATGGAAAACGGCGAAGCCTTTCCTGGAGTCGTGGATTAAAGATCAGGTCGGTATTCC  >  NC_000913/4021289‑4021556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑