Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,559,878	C→T	A41A (GCG→GCA)	yjiG ←	Gate family protein YjiG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,559,878	0	C	T	100.0%	290.6 / NA	83	A41A (GCG→GCA)	yjiG	Gate family protein YjiG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (40/43);  total (40/43)

GCGGCAACACCGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTC  >  NC_000913/4559741‑4560010                                                                                                                                          |                                                                                                                                     gcggcAACACCGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAgtgc                                                                                                                                    <  2:172771/140‑1 (MQ=255)  cggcAACACCGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGTTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGcc                                                                                                                                   <  2:1343784/140‑1 (MQ=255)  cggcAACACCGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGcc                                                                                                                                   <  2:1369286/140‑1 (MQ=255)   ggcAACACCGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCt                                                                                                                                  >  2:88316/1‑140 (MQ=255)     cAACACCGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGa                                                                                                                                <  2:1213029/140‑1 (MQ=255)           cGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAatgatg                                                                                                                          >  1:711049/1‑140 (MQ=255)               tGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACaa                                                                                                                      <  1:202240/140‑1 (MQ=255)                gcgcCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACaaa                                                                                                                     >  2:474532/1‑140 (MQ=255)                  gcCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACg                                                                                                                   >  1:653645/1‑140 (MQ=255)                  gcCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACg                                                                                                                   >  1:760509/1‑140 (MQ=255)                   ccACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGc                                                                                                                  >  2:786100/1‑140 (MQ=255)                      cccATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCAt                                                                                                               <  2:1321768/140‑1 (MQ=255)                          tACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATcacc                                                                                                           <  2:381544/140‑1 (MQ=255)                           aCTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACa                                                                                                                       <  1:666081/127‑1 (MQ=255)                           aCTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACa                                                                                                                       >  2:666081/1‑127 (MQ=255)                               aTTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGtt                                                                                                      <  1:383485/140‑1 (MQ=255)                                 tAATGTGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTg                                                                                                    >  2:1060469/1‑140 (MQ=255)                                 tAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTg                                                                                                    >  1:77997/1‑140 (MQ=255)                                 tAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTg                                                                                                    >  1:1031842/1‑140 (MQ=255)                                 tAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTg                                                                                                    >  2:247273/1‑140 (MQ=255)                                 tAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTg                                                                                                    >  1:1381938/1‑140 (MQ=255)                                  aaTGCGGCTAAAAGGACGGTTGCTGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTgg                                                                                                   >  1:546230/1‑140 (MQ=255)                                  aaTGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTgg                                                                                                   <  1:786100/140‑1 (MQ=255)                                    tGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGgca                                                                                                 >  2:1200740/1‑140 (MQ=255)                                    tGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGgca                                                                                                 >  1:1301727/1‑140 (MQ=255)                                     gCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGgcag                                                                                                <  2:955914/140‑1 (MQ=255)                                      cGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGgcagc                                                                                               >  2:110092/1‑140 (MQ=255)                                       ggCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGgcagca                                                                                              >  2:286072/1‑140 (MQ=255)                                         cTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAgg                                                                                            >  1:271220/1‑140 (MQ=255)                                         cTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAgg                                                                                            >  2:825212/1‑140 (MQ=255)                                          tAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGt                                                                                           <  2:271220/140‑1 (MQ=255)                                          tAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGt                                                                                           <  1:1091483/140‑1 (MQ=255)                                                  cGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCATGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTg                                                                                   >  2:1308524/1‑140 (MQ=255)                                                  cGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTg                                                                                   >  1:1375935/1‑140 (MQ=255)                                                    gTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGc                                                                                 <  2:760509/140‑1 (MQ=255)                                                     ttGCTGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCg                                                                                <  2:546230/140‑1 (MQ=255)                                                     ttGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCg                                                                                <  2:575438/140‑1 (MQ=255)                                                     ttGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCg                                                                                <  1:392592/140‑1 (MQ=255)                                                     ttGCCGCTTCACCGGGTAATCCCCACAGCGCCACTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCg                                                                                <  2:1031842/140‑1 (MQ=255)                                                       gCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGAt                                                                              <  1:453389/140‑1 (MQ=255)                                                       gCCGCATCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGAt                                                                              >  2:430624/1‑140 (MQ=255)                                                               aCCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGc                                                                      >  1:899126/1‑140 (MQ=255)                                                               aCCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGc                                                                      >  2:755621/1‑140 (MQ=255)                                                               aCCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAATCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTc                                                                                      <  1:789463/124‑1 (MQ=255)                                                               aCCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAATCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTc                                                                                      >  2:789463/1‑124 (MQ=255)                                                                 cGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCa                                                                    <  2:103352/140‑1 (MQ=255)                                                                  gggTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCAc                                                                   >  1:1300581/1‑140 (MQ=255)                                                                         ccccACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgc                                                            <  1:1060469/140‑1 (MQ=255)                                                                          cccACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgcg                                                           <  1:474532/140‑1 (MQ=255)                                                                          cccACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgcg                                                           <  1:825212/140‑1 (MQ=255)                                                                           ccACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgcgc                                                          <  2:1301727/140‑1 (MQ=255)                                                                            cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                         <  2:899126/140‑1 (MQ=255)                                                                            cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                         <  2:1142003/140‑1 (MQ=255)                                                                            cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                         <  1:247273/140‑1 (MQ=255)                                                                            cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                         <  2:711049/140‑1 (MQ=255)                                                                            cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                         <  2:1300581/140‑1 (MQ=255)                                                                            cacaGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGcc                                                         <  1:88316/140‑1 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgcgc                                                          <  1:669662/134‑1 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGgcgcgcgc                                                          >  2:669662/1‑134 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGa                                                    >  1:1363272/1‑140 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGa                                                    >  1:813599/1‑140 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGa                                                    <  1:755621/140‑1 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGa                                                    <  2:813599/140‑1 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGa                                                    <  1:286072/140‑1 (MQ=255)                                                                                 cgcCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGa                                                    >  1:599845/1‑140 (MQ=255)                                                                                   ccATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATa                                                  >  2:782488/1‑140 (MQ=255)                                                                                         cGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACata                                            >  2:355044/1‑140 (MQ=255)                                                                                         cGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACata                                            <  1:355044/140‑1 (MQ=255)                                                                                                 caGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATa                                                  >  2:421110/1‑126 (MQ=255)                                                                                                 caGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATa                                                  <  1:421110/126‑1 (MQ=255)                                                                                                        gCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTg                             <  2:1008102/140‑1 (MQ=255)                                                                                                          cccacccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCg                           <  2:1036073/140‑1 (MQ=255)                                                                                                             acccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAc                        <  2:653645/140‑1 (MQ=255)                                                                                                             acccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAc                        <  2:640221/140‑1 (MQ=255)                                                                                                              cccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                       >  1:872975/1‑140 (MQ=255)                                                                                                              cccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                       >  1:476655/1‑140 (MQ=255)                                                                                                              cccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                       >  2:245814/1‑140 (MQ=255)                                                                                                              cccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                       >  2:1098397/1‑140 (MQ=255)                                                                                                              cccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                       >  2:1285161/1‑140 (MQ=255)                                                                                                              cccaGTCGAGCAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGAcc                       >  2:309799/1‑140 (MQ=255)                                                                                                                        cAGGCCGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCa             <  2:1375935/140‑1 (MQ=255)                                                                                                                             cGGTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCtt        >  2:449928/1‑140 (MQ=255)                                                                                                                               gTAATTTTCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTgc      <  1:245814/140‑1 (MQ=255)                                                                                                                                   ttttCAGTGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTc  <  1:449928/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     GCGGCAACACCGACTGCGCCACCCATACTCATTAATGCGGCTAAAAGGACGGTTGCCGCTTCACCGGGTAATCCCCACAGCGCCATTACGGGTTCACAGATATGCCCCACCCAGTCGAGCAGGCCGGTAATTTTCAGCGCCTGAATGATGACAAACGCCATCACCACGTTTGGCAGCAGGTTGGTCGTGGCGATAGTAAAGCCACGGCGCGCGCCATCGATAAACATATCCATGACGTTTTTGCGGACCTGAGTTGTCATGCTTGCGCTC  >  NC_000913/4559741‑4560010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑