Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,379,399	G→A	D506N (GAC→AAC)	ycjT →	kojibiose phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,379,399	0	G	A	100.0%	97.8 / NA	28	D506N (GAC→AAC)	ycjT	kojibiose phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/12);  total (16/12)

CGATCGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCGACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAA  >  NC_000913/1379263‑1379523                                                                                                                                         |                                                                                                                             cGATCGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGcaacg                                                                                                                           >  2:420989/1‑140 (MQ=255)    tCGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATg                                                                                                                        <  1:309689/140‑1 (MQ=255)     cGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGc                                                                                                                       >  1:96560/1‑140 (MQ=255)                                  aGACGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCa                                                                                          <  1:150267/140‑1 (MQ=255)                                  aGACGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCa                                                                                          >  2:272920/1‑140 (MQ=255)                                    aCGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCaaa                                                                                        <  1:420989/140‑1 (MQ=255)                                       aaTATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAagag                                                                                     <  1:272920/140‑1 (MQ=255)                                                aaCATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATg                                                                            >  1:243022/1‑140 (MQ=255)                                                     gTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGa                                                                       >  1:263388/1‑140 (MQ=255)                                                     gTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGa                                                                       >  2:379933/1‑140 (MQ=255)                                                               atGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGccc                                                             >  1:374419/1‑140 (MQ=255)                                                                tGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCg                                                            >  1:220916/1‑140 (MQ=255)                                                                tGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCg                                                            >  1:13622/1‑140 (MQ=255)                                                                    tACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGa                                                           <  1:57679/137‑1 (MQ=255)                                                                    tACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGa                                                           >  2:57679/1‑137 (MQ=255)                                                                    tACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGa                                                           >  2:149422/1‑137 (MQ=255)                                                                    tACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGa                                                           <  1:149422/137‑1 (MQ=255)                                                                            cTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGc                                                >  2:113022/1‑140 (MQ=255)                                                                              atatGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCg                                              <  2:263388/140‑1 (MQ=255)                                                                                 tGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGc                                             >  2:244582/1‑138 (MQ=255)                                                                                 tGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGc                                             <  1:244582/138‑1 (MQ=255)                                                                                  ggCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAgg                                          <  2:220916/140‑1 (MQ=255)                                                                                            agctataaaCAGGCGCAAATTATTCCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGtt                                <  2:13622/133‑1 (MQ=255)                                                                                             aCGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGttt                               <  1:379933/140‑1 (MQ=255)                                                                                                        gcgcTGAATATTGCCCGCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGcc                    >  2:457634/1‑140 (MQ=255)                                                                                                                        gCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCg    >  1:54219/1‑140 (MQ=255)                                                                                                                        gCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCg    <  1:424476/140‑1 (MQ=255)                                                                                                                        gCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCg    <  2:243022/140‑1 (MQ=255)                                                                                                                        gCCAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCg    >  1:345401/1‑140 (MQ=255)                                                                                                                          cAGTTCGGCTGTAGCAACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGaa  >  1:93235/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                             CGATCGTCTGGAAATTCATGATGTTATTGGGCCAGACGAATATACCGAACATGTCAATAATAATGCATACACCAGCTATATGGCCCGCTACAACGTTCAACAGGCGCTGAATATTGCCCGCCAGTTCGGCTGTAGCGACGATGCGTTTATCCATCGCGCCGAAATGTTCCTCAAAGAGCTATGGATGCCAGAAATTCAGCCCGACGGCGTTTTGCCGCAGGATGATTCGTTTATGGCTAAGCCGGCGATTAATCTGGCGAA  >  NC_000913/1379263‑1379523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑