Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,330,459	T→G	intergenic (+221/‑43)	yjcZ → / → proP	uncharacterized protein YjcZ/osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,330,459	0	T	G	100.0%	190.2 / NA	49	intergenic (+221/‑43)	yjcZ/proP	uncharacterized protein YjcZ/osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (28/21);  total (28/21)

ATTAACTGCCCAATTCAGGCGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAG  >  NC_000913/4330323‑4330589                                                                                                                                         |                                                                                                                                   aTTAACTGCCCAATTCAGGCGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTc                                                                                                                                 >  2:441806/1‑140 (MQ=255) aTTAACTGCCCAATTCAGGCGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTc                                                                                                                                 >  2:268161/1‑140 (MQ=255)        gCCCAATTCAGGCGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGtt                                                                                                                          >  2:396780/1‑140 (MQ=255)                cAGGCGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGaaa                                                                                                                  <  1:425117/140‑1 (MQ=255)                    cGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGaaa                                                                                                                  >  1:226717/1‑136 (MQ=255)                    cGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGaaa                                                                                                                  <  2:226717/136‑1 (MQ=255)                        aaCTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCg                                                                                                                            >  2:352162/1‑122 (MQ=255)                        aaCTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCg                                                                                                                            <  1:352162/122‑1 (MQ=255)                                 gATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAg                                                                                                 <  1:268161/140‑1 (MQ=255)                                 gATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAg                                                                                                 <  1:441806/140‑1 (MQ=255)                                    tGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGaa                                                                                              >  1:127377/1‑140 (MQ=255)                                            ccTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGc                                                                                      >  2:437195/1‑140 (MQ=255)                                                     gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                             >  2:339031/1‑140 (MQ=255)                                                     gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                             >  2:278246/1‑140 (MQ=255)                                                     gAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGa                                                                             >  1:8423/1‑140 (MQ=255)                                                       gggTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGaaa                                                                           >  2:124847/1‑140 (MQ=255)                                                               gCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTaaa                                                                   <  1:396780/140‑1 (MQ=255)                                                                cAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTaaaa                                                                  >  1:345909/1‑140 (MQ=255)                                                                  aaCCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAAcc                                                                >  2:87796/1‑140 (MQ=255)                                                                   aCCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCg                                                               >  1:439378/1‑140 (MQ=255)                                                                    cccGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGa                                                              >  1:283336/1‑140 (MQ=255)                                                                    cccGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGa                                                              >  1:183147/1‑140 (MQ=255)                                                                    cccGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGa                                                              >  2:246732/1‑140 (MQ=255)                                                                     ccGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGAt                                                             <  2:183147/140‑1 (MQ=255)                                                                     ccGCTACGCTTGTTACAGAGATTGCATCCTGCAATCCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGACGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGAt                                                             <  2:342878/140‑1 (MQ=255)                                                                        cTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTAc                                                          >  1:165045/1‑140 (MQ=255)                                                                         tACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTAcc                                                         >  1:304304/1‑140 (MQ=255)                                                                             cTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTc                                                     <  1:246732/140‑1 (MQ=255)                                                                                 ttACAGAGATTGAATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGAGCTGATTGTTCTGGCGTCTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGa                                                 <  2:453189/140‑1 (MQ=255)                                                                                  tACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGAt                                                <  1:319375/140‑1 (MQ=255)                                                                                        gaTTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCAcc                                          >  2:395554/1‑140 (MQ=255)                                                                                         aTTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCa                                         <  2:345909/140‑1 (MQ=255)                                                                                          ttGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGt                                              >  1:302280/1‑134 (MQ=255)                                                                                          ttGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGt                                              <  2:302280/134‑1 (MQ=255)                                                                                           tGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCatt                                       >  2:322321/1‑140 (MQ=255)                                                                                                ccTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATtga                                  <  2:304304/140‑1 (MQ=255)                                                                                                   gCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGTAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATtgatga                               >  2:270677/1‑140 (MQ=255)                                                                                                   gCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGAt                                                >  1:145383/1‑123 (MQ=255)                                                                                                   gCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGAt                                                <  2:145383/123‑1 (MQ=255)                                                                                                   gCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATtgatga                               >  2:134388/1‑140 (MQ=255)                                                                                                           cGCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAAc                       <  1:339031/140‑1 (MQ=255)                                                                                                            gCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCGTCGTGATGTCACCATTATTGATGACGGTAAACt                      >  1:54652/1‑140 (MQ=255)                                                                                                            gCTCCCCTTTTGCGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAAGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACt                      >  2:113594/1‑140 (MQ=255)                                                                                                                        cGGCCGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCAt          >  2:2647/1‑140 (MQ=255)                                                                                                                           ccGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTAc       <  1:437195/140‑1 (MQ=255)                                                                                                                           ccGTCGCGCTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTAc       <  1:124847/140‑1 (MQ=255)                                                                                                                                gcgcTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag  <  2:283336/140‑1 (MQ=255)                                                                                                                                gcgcTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag  <  1:134388/140‑1 (MQ=255)                                                                                                                                gcgcTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag  <  2:54652/140‑1 (MQ=255)                                                                                                                                gcgcTGATTGTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCgcag  <  1:395554/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   ATTAACTGCCCAATTCAGGCGTCAACTGGTTTGATTGTACATTCCTTAACCGGAGGGTGTAAGCAAACCCGCTACGCTTGTTACAGAGATTGCATCCTGCAATTCCCGCTCCCCTTTTGCGGCCGTCGCGCTGATTTTTCTGGCGTTTGCGGAAATGGGCCAACTCTGCGAGGAAAGCTATGCTGAAAAGGAAAAAAGTAAAACCGATTACCCTTCGTGATGTCACCATTATTGATGACGGTAAACTGCGTAAAGCCATTACCGCAG  >  NC_000913/4330323‑4330589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑