Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	897,437	C→T	G118G (GGC→GGT)	potI →	putrescine ABC transporter membrane subunit PotI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	897,437	0	C	T	100.0%	45.0 / NA	14	G118G (GGC→GGT)	potI	putrescine ABC transporter membrane subunit PotI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/8);  total (6/8)

TGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGC  >  NC_000913/897305‑897573                                                                                                                                     |                                                                                                                                         tgCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGGGGGGGTGGTGCGCTTTGGGAGGTTTCGCGGAACCAATGGCTTTGCCTTTATGATCACCGCGCCCCTGGTAATGCCAGATGTCATAACGGGTTTGTTGc                                                                                                                                   >  2:240467/1‑140 (MQ=255)   cggcgACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCtg                                                                                                                                 <  1:240467/140‑1 (MQ=255)                            aaCTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTc                                                                                                        <  1:262263/140‑1 (MQ=255)                            aaCTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTc                                                                                                        <  1:302896/140‑1 (MQ=255)                               tATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTc                                                                                                        >  2:359686/1‑137 (MQ=255)                               tATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTc                                                                                                        <  1:359686/137‑1 (MQ=255)                               tATTGCGGCGGTGGTGCTGGGGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGGAATGCCCGATGTCATCACGGGGTTGTCGCTGGTGTTATTATTTGTCGCGCTTGCTCATg                                                                                                     >  2:302896/1‑140 (MQ=255)                                                tggtgcGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCg                                                                                    <  2:97810/140‑1 (MQ=255)                                                                                                    cgcgCCGCTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCtt                                >  1:427510/1‑140 (MQ=255)                                                                                                      cgccgcTGGTAATGCCAGATGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTAt                              >  2:143673/1‑140 (MQ=255)                                                                                                                        aTGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATttct            >  2:129139/1‑140 (MQ=255)                                                                                                                        aTGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATttct            >  1:265915/1‑140 (MQ=255)                                                                                                                        aTGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATttct            >  2:409776/1‑140 (MQ=255)                                                                                                                         tGTCATCACGGGTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATttctt           >  2:85318/1‑140 (MQ=255)                                                                                                                                  gggTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGc  <  1:85318/140‑1 (MQ=255)                                                                                                                                  gggTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGc  <  1:409776/140‑1 (MQ=255)                                                                                                                                  gggTTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGc  <  1:129139/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                         TGCGGCGACGGCGGCGGCGATCCTCGGAACTATTGCGGCGGTGGTGCTGGTGCGCTTTGGCAGGTTTCGCGGATCAAATGGCTTTGCCTTTATGATCACCGCGCCGCTGGTAATGCCAGATGTCATCACGGGCTTGTCGCTGTTGTTATTATTCGTCGCGCTTGCTCATGCCATTGGCTGGCCTGCGGACCGCGGTATGCTCACCATCTGGCTGGCGCATGTCACTTTTTGTACGGCTTATGTGGCGGTCGTTATTTCTTCAAGATTGC  >  NC_000913/897305‑897573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑