BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,181,120	G→A	L50F (CTT→TTT)	ycfZ ←	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,181,120	0	G	A	100.0%	124.4 / NA	36	L50F (CTT→TTT)	ycfZ	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/19); total (17/19)

AACACTTCCTTTTACATCACTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAGCTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAG  >  NC_000913/1180983‑1181246

aaCACTTCCTTTTACATCACTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACt                                                                                                                              >  2:386972/1‑140 (MQ=255)

        cTTTTACATCACTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTa                                                                                                                      >  1:164053/1‑140 (MQ=255)

          tttACATCACTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATg                                                                                                                    >  2:421734/1‑140 (MQ=255)

              cATCACTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGcgcg                                                                                                                <  1:251096/140‑1 (MQ=255)

                 cACTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGAtc                                                                                                             >  2:360838/1‑140 (MQ=255)

                   cTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGAtctc                                                                                                           >  2:341116/1‑140 (MQ=255)

                   cTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGAtctc                                                                                                           >  2:18847/1‑140 (MQ=255)

                   cTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGAtctc                                                                                                           >  2:452322/1‑140 (MQ=255)

                   cTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGAtctc                                                                                                           >  1:169990/1‑140 (MQ=255)

                     cAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCtt                                                                                                         <  1:386972/140‑1 (MQ=255)

                      aaTGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTc                                                                                                        >  1:107397/1‑140 (MQ=255)

                       aTGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCa                                                                                                       <  2:317195/140‑1 (MQ=255)

                       aTGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCa                                                                                                       <  2:426469/140‑1 (MQ=255)

                        tGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAg                                                                                                      >  2:255844/1‑140 (MQ=255)

                            gATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATc                                                                                                  <  1:114786/140‑1 (MQ=255)

                                   cATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACa                                                                                           <  2:132136/140‑1 (MQ=255)

                                         ccAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCg                                                                                     >  1:319345/1‑140 (MQ=255)

                                               tCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACg                                                                               <  1:318033/140‑1 (MQ=255)

                                                          ttttGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAg                                                                    >  1:466515/1‑140 (MQ=255)

                                                           tttGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAgt                                                                   <  1:421734/140‑1 (MQ=255)

                                                             tGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAgtgt                                                                 <  1:341116/140‑1 (MQ=255)

                                                               tCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCt                                                               >  1:274366/1‑140 (MQ=255)

                                                                         gCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTgg                                                     <  1:376128/140‑1 (MQ=255)

                                                                          cTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGa                                                    >  2:101048/1‑140 (MQ=255)

                                                                                    aaTGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAAt                                              >  1:307770/1‑136 (MQ=255)

                                                                                    aaTGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAAt                                              <  2:307770/136‑1 (MQ=255)

                                                                                             tAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTgctggctg                                 <  2:164053/140‑1 (MQ=255)

                                                                                                  ccACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTc                            <  1:381474/140‑1 (MQ=255)

                                                                                                       aTAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAgg                       >  2:401494/1‑140 (MQ=255)

                                                                                                        tAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGc                      <  2:274366/140‑1 (MQ=255)

                                                                                                         aaaCGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCa                     <  1:101048/140‑1 (MQ=255)

                                                                                                          aaCGGTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAg                    <  2:466515/140‑1 (MQ=255)

                                                                                                             ggTGCAATTCCCGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAg                 <  1:401494/140‑1 (MQ=255)

                                                                                                                       ccGTTTGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTaaa       >  1:106690/1‑140 (MQ=255)

                                                                                                                          tttGTTGGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTg    <  1:360838/140‑1 (MQ=255)

                                                                                                                            tgttgGCGAAAAAACTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAg  <  2:106690/140‑1 (MQ=255)

AACACTTCCTTTTACATCACTCAATGAAGATGACGCATGCGCCAGCATCGCTTTACCATTTTGTCTGAGACTGGCTTCATTATTAATGTCGGGTAAAACCACAATAAACGGTGCAATTCCCGTTTGTTGGCGAAAAAGCTCGGCTTTATGCGCGATCTCTTCAGCATCTGGTACAGTACCGGTAACGTCCGTAAAAAGTGTCTTCATTATTGGAATTAATGGCTTGCTGGCTGCTGTCAGAGGCAGGAGTATCAGTAAACTGAG  >  NC_000913/1180983‑1181246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`