Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	445,558	0	A	C	24.4%	84.3 / 12.1	46	V370G (GTA→GGA)	yajR	putative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (26/8);  new base C (0/11);  total (26/20)
Fisher's exact test for biased strand distribution p-value = 7.45e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGG  >  NC_000913/445425‑445697                                                                                                                                      |                                                                                                                                            aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                       <  1:456573/140‑1 (MQ=255) aCGCACTTTTAACGCCTCATTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATTCCCCCt                                                                                                                                       <  2:466453/140‑1 (MQ=255)  cGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTg                                                                                                                                      <  1:307465/140‑1 (MQ=255)   gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                     <  1:656734/140‑1 (MQ=255)   gCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGa                                                                                                                                     <  1:637915/140‑1 (MQ=255)              gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                          <  2:405862/140‑1 (MQ=255)                 tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTTCGCCAGCGAGAAAAAACCCCTGGCCCGCCAACATGcc                                                                                                                       >  2:385578/1‑140 (MQ=255)                 tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGGCCGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAAAAACCCCTGACCGTCCAACATGcc                                                                                                                       >  2:457856/1‑140 (MQ=255)                   gTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGt                                                                                                                     >  2:683781/1‑140 (MQ=255)                           cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAg                                                                                                             <  2:319751/140‑1 (MQ=255)                           cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAg                                                                                                             <  1:683781/140‑1 (MQ=255)                                        gATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAAc                                                                                                <  1:978654/140‑1 (MQ=255)                                            tCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGcc                                                                                            <  1:385578/140‑1 (MQ=255)                                                  cgcAACCGGCTGACATCCGGCGTTTCTTTCAGGGTCCTGGCGACTGTCAGCCACCCTGCGGCCAGCTTTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                      <  2:772091/140‑1 (MQ=255)                                                  cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCCCCCAGCGAACCGCCAATcgc                                                                                      >  1:1269907/1‑140 (MQ=255)                                                  cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGGTAATCCAGCCGCCCCGCGAACCGCCCATcgc                                                                                      >  1:402913/1‑140 (MQ=255)                                                  cgcAAACTGCTGACATACGGCGGTTCTTTCATGGGACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCCCCCCGCGAACCGCCAATcgc                                                                                      >  2:40825/1‑140 (MQ=255)                                                   gcAAACTGCTGACATACGGCGGTTCTTTTATGGTACTTGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCCCCGAGAAAAACCCCCCGACCCTCAAACAAGCCCTTAATCCCGCCGCCCCGCGAACCGCCCATcgcc                                                                                     >  2:388395/1‑140 (MQ=255)                                                    cAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTGCGCCCGCGAGAAAAAACCCCCGACCGGCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCGCCAATcgccc                                                                                    >  2:676067/1‑139 (MQ=255)                                                    cAAACTGCTGACATACGGCGGTTCTTTCATGGTACTTGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgccc                                                                                    >  1:979000/1‑139 (MQ=255)                                                    aaacctgcggACATACGGGGTTTTTTTCATGGTACGGGCGCCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                                    <  2:266840/136‑1 (MQ=255)                                                            tgACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCCGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGa                                                                            >  1:681471/1‑140 (MQ=255)                                                              aCATACGGCGTTTTTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCCCCGGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                          <  2:129664/140‑1 (MQ=255)                                                                aTACGGCGTTTTTTTCATGGTACGGGCGACTGTCAGCCACACTGCGGCAAGCATTGCGCCAGCGAAAAATCCCCCCGGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAAct                                                                        <  2:979000/140‑1 (MQ=255)                                                                     gcgtTTTTTTCATGGTACTGGCGCCTGTCACCCCCACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCTGTCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggctg                                                                   <  2:681471/140‑1 (MQ=255)                                                                      cggTTCTTTCATGGTACTGGCGCCTGTCAGCCACACTGCGGCCAGATTTGCGCCAGCGAAAAATACCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggctgg                                                                  <  2:496790/140‑1 (MQ=255)                                                                        gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGGTAATCCAGCCGCCCCGCGAACCGCCAATCGCCACGCCCAAAAAcaggctgggg                                                                >  2:370841/1‑138 (MQ=255)                                                                        gTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                                >  1:852534/1‑140 (MQ=255)                                                                         ttCTTTTATGGTACTGGCGACTGTCAGCCACACCGCGGCCCGCATTGCGCCAGCGGGAAAAAACCCCCCACCCTCAAACATGCCGTTAATCCAGCCCCCCCGCGAACCCCCCATCGCCACCCCCAGAAACAGGCtggggg                                                               >  2:146056/1‑140 (MQ=255)                                                                                  ggTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAacac                                                      >  1:691916/1‑140 (MQ=255)                                                                                   gTACTGGCGCCTGTCAGCCACACTGCGGCCAGCTTGGCGCCGGCGAGAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                     <  1:329748/140‑1 (MQ=255)                                                                                      cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                                  >  1:619257/1‑140 (MQ=255)                                                                                      cTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCAt                                                  >  1:584683/1‑140 (MQ=255)                                                                                        ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                                >  1:335725/1‑140 (MQ=255)                                                                                         gCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                               >  1:1205233/1‑140 (MQ=255)                                                                                           gcctttCACCCACCCGGCGGCAAGCTTGGCCCCGGCAAAAAATCCCCCCGCCCCTCCAAACATGCCGTTAAGCCACCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                             <  2:907780/135‑1 (MQ=255)                                                                                            ccTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                            <  1:146056/139‑1 (MQ=255)                                                                                            ccTGTCAGCACCCCGGCGGCCGGCTTTGCCCAAGGAAGAAATACCCCCGGCCCTAAAAACATGCCGTTAACCCAGCCCCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                            <  2:298084/139‑1 (MQ=255)                                                                                            aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                            >  1:857666/1‑140 (MQ=255)                                                                                             ctgccgtccACACTGCGCCCAGCATTCCCCGAGCGAAAAAACCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                           <  2:699889/133‑1 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                          >  1:732616/1‑140 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                          >  2:64738/1‑140 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                          >  1:783313/1‑140 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                          >  2:384212/1‑140 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                          >  1:907780/1‑140 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                          >  2:180700/1‑140 (MQ=255)                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                          >  2:912768/1‑140 (MQ=255)                                                                                                tCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCt                                        <  1:40825/140‑1 (MQ=255)                                                                                                  aGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                           <  1:612376/135‑1 (MQ=255)                                                                                                  aGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                           >  2:612376/1‑135 (MQ=255)                                                                                                   gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                                     >  1:516276/1‑140 (MQ=255)                                                                                                       cacTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAc                                 >  1:699889/1‑140 (MQ=255)                                                                                                          tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                              >  1:1221737/1‑140 (MQ=255)                                                                                                          tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                              >  1:697936/1‑140 (MQ=255)                                                                                                              gCCAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                          <  1:676067/140‑1 (MQ=255)                                                                                                               ccAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                         <  1:180700/140‑1 (MQ=255)                                                                                                                 agaaTTGCGCCGGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                       <  2:335725/137‑1 (MQ=255)                                                                                                                 aGCTTTGCGCAAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                       <  2:857666/140‑1 (MQ=255)                                                                                                                 aGCTTTGCCCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                       <  2:1221737/140‑1 (MQ=255)                                                                                                                 aGCATTGCGCCAGCAAAAAACCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                       <  2:402913/140‑1 (MQ=255)                                                                                                                  gCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                      >  1:855824/1‑140 (MQ=255)                                                                                                                  gCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                      >  1:908077/1‑140 (MQ=255)                                                                                                                  gCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                      <  2:1205233/140‑1 (MQ=255)                                                                                                                  gCATGGCCCCAGCGAAAAACCCCCCCTCCCCGCCAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAc                      <  2:584683/140‑1 (MQ=255)                                                                                                                   cATTGCGCCAGCGAGAAATCCCCCCTGACCGTAAAACATGCCTTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct                     <  1:101554/140‑1 (MQ=255)                                                                                                                       gcgcCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                 <  2:852534/140‑1 (MQ=255)                                                                                                                       gccccAGCGACAAATCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGCAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt                 <  2:908395/137‑1 (MQ=255)                                                                                                                            aGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa            <  1:912768/140‑1 (MQ=255)                                                                                                                                 aaaaTCCCCCCTGCCCGTAAAAAACGCCGTTAACCCACCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt       <  2:619257/139‑1 (MQ=255)                                                                                                                                      aCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAggg  >  2:595177/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                            ACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGAGGG  >  NC_000913/445425‑445697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑